Over‐expressed rice ADP‐ribosylation factor 1 (RARF1) induces pathogenesis‐related genes and pathogen resistance in tobacco plants

Lee, Won Young; Hong, Joon Ki; Kim, Cha Young; Chun, Hyun Jin; Park, Hyeong Cheol; Kim, Jong Cheol; Yun, Dae‐Jin; Chung, Woo Sik; Lee, Sungho; Lee, Sang Yeol; Cho, Moo Je; Lim, Chae Oh

doi:10.1046/j.1399-3054.2003.00215.x

Cited by 36 publications

(25 citation statements)

References 48 publications

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“…Constitutive overexpression of RARF1 in N. tabacum triggered the formation of spontaneous lesions, induced pathogenesis‐related genes and resulted in increased resistance towards the oomycete pathogen Phytophthora parasitica var. nicotianae (Lee et al ., 2003). However, the authors did not suggest a possible molecular mechanism to explain these observations.…”

Section: Discussionmentioning

confidence: 99%

“…(2006) reported that the Arabidopsis ARF-GEF AtMIN7 is specifically targeted by HopM1, a Pseudomonas syringae virulence factor. However, to our knowledge, a direct association of ARF1 with disease response in plants has been reported only once for a rice ARF1: RARF1 (Lee et al ., 2003). Although the authors did not report any loss-of-function data, they showed a rapid pathogen-induced increase in gene expression of RARF1.…”

Section: Introductionmentioning

confidence: 93%

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High‐throughput in planta expression screening identifies an ADP‐ribosylation factor (ARF1) involved in non‐host resistance and R gene‐mediated resistance

Coemans¹,

Takahashi²,

Berberich³

et al. 2007

Molecular Plant Pathology

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To identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus X-based overexpression in planta of a cDNA library derived from paraquat-treated Nicotiana benthamiana leaves. The screening of 30,000 cDNA clones enabled the identification of an ADP-ribosylation factor 1 (ARF1) that induces cell death when overexpressed in N. benthamiana. Overexpression of the guanosine diphosphate (GDP)-locked mutant of ARF1 did not trigger cell death, suggesting that ARF1 guanosine triphosphatase (GTPase) activity is necessary for the observed cell death-inducing activity. The ARF1 transcript level increased strongly following treatment with Phytophthora infestans elicitor INF1, as well as inoculation with a non-host pathogen Pseudomonas cichorii in N. benthamiana. In addition, ARF1 was induced in the interaction between the N gene and tobacco mosaic virus (TMV) in Nicotiana tabacum. By contrast, inoculation with the virulent pathogen Pseudomonas syringae pv. tabaci did not affect ARF1 expression in N. benthamiana. Virus-induced gene silencing of ARF1 in N. benthamiana resulted in a stunted phenotype, and severely hampered non-host resistance towards P. cichorii. In addition, ARF1 silencing partially compromised resistance towards TMV in N. benthamiana containing the N resistance gene. By contrast, and in accordance with the ARF1 gene expression profile, silencing of ARF1 transcription did not alter the susceptibility of N. benthamiana towards the pathogen P. syringae pv. tabaci. These results strongly implicate ARF1 in the non-host resistance to bacteria and N gene-mediated resistance in N. benthamiana.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 93%

High‐throughput in planta expression screening identifies an ADP‐ribosylation factor (ARF1) involved in non‐host resistance and R gene‐mediated resistance

Coemans¹,

Takahashi²,

Berberich³

et al. 2007

Molecular Plant Pathology

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“…, 2004). A notable aspect of plant ARF proteins is a clear involvement of OsARF1 in plant defense, enhancing disease resistance against pathogens with concomitant induction of SA‐activated PR genes (Lee et al. , 2003).…”

Section: Discussionmentioning

confidence: 99%

“…, 2002). Rice ARF was suggested to be involved in disease response, as shown by analyses of transgenic tobacco plants that exhibited fungal pathogen resistance and rapid increase of pathogen‐responsive gene expression (Lee et al. , 2003).…”

Section: Introductionmentioning

confidence: 99%

Attenuation of the hypersensitive response by an ATPase associated with various cellular activities (AAA) protein through suppression of a small GTPase, ADP ribosylation factor, in tobacco plants

Lee

Sano²

2007

The Plant Journal

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SummaryATPase associated with various cellular activities (AAA) proteins are commonly distributed among eukaryotes, and are involved in a multitude of cellular functions. NtAAA1 is one such example, being involved in pathogen response in tobacco plants. When its activity was suppressed in RNAi transgenic tobacco plants, an elevated resistance to the pathogenic bacterium Pseudomonas syringae was observed in comparison with the wild type. As AAA proteins function through interaction with specific partners, NtAAA1-interacting proteins were screened by the yeast two-hybrid assay, and one particular gene encoding a small GTPase, an ADP ribosylation factor, was identified and designated as NtARF. Its specific binding to NtAAA1 was confirmed by in vitro pulldown assay, and their interaction was predominant between active forms of NtARF and NtAAA1, each bound to GTP and ATP, respectively. Their physical interaction in vivo around the plasma membrane was shown by fluorescence resonance energy transfer assays, suggesting their role in membrane trafficking. Transgenic tobacco plants constitutively expressing NtARF under the control of a cauliflower mosaic virus 35S promoter exhibited spontaneous and wound-induced lesion formation, and enhanced resistance to pathogen attack. Expression of NtAAA1 in leaves of NtARF transgenic plants attenuated lesion and suppressed pathogen resistance. In wild-type tobacco plants, transcripts of NtAAA1 and NtARF could be induced by ethylene and salicylic acid, respectively. These results suggest that NtAAA1 balances plant resistance through suppression of NtARF, and that the molecular basis for the known antagonistic actions of ethylene and salicylic acid in defense response could be partly attributable to these two proteins.

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“…Furthermore, we identified an ADP‐ribosylation factor (ARF) which is a member of a multigene family of 20 kDa guanine nucleotide binding proteins (Moss & Vaughan, 1999; Lee et al., 2003), and is also therefore likely to be involved in signal transduction pathways. ARFs are components of various plant defence signalling pathways involved in the induction of expression of a subset of PR genes (Lee et al., 2003) or are assumed to have key biological functions in defensive cytotoxicity, pathogenicity, intracellular signalling, DNA repair, and cell division (Han et al., 1999). They were also found to be differentially expressed in rice cells treated with a fungal elicitor prepared from the rice blast fungus Magnaporthe grisea (T.T.…”

Section: Discussionmentioning

confidence: 99%

Molecular interactions between rosy apple aphids, Dysaphis plantaginea, and resistant and susceptible cultivars of its primary host Malus domestica

Qubbaj

Reineke

Zebitz

2005

Entomologia Exp Applicata

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The use of crop varieties resistant or tolerant to insect pests or other stress factors is one approach in non-chemical crop-protection. Knowledge of the biochemical and molecular background of insectplant interactions is a prerequisite for optimizing breeding for resistance. However, the resistance genes involved in plant-aphid interactions have so far only been identified and characterized in very few plant species. Our work aims to elucidate the molecular and biochemical mechanisms involved in resistance of apple trees, Malus domestica L. (Rosaceae), against its primary aphid pest, the rosy apple aphid, Dysaphis plantaginea (Passerini) (Homoptera: Aphididae), which is considered a serious economic pest of apple. Gene expression in both resistant and susceptible apple cultivars after infestation with rosy apple aphids was investigated by employing the cDNA-AFLP method (cDNAAmplified Fragment Length Polymorphism). From approximately 12 500 cDNA fragments detected on polyacrylamide gels, 21 bands were apparently up-or down-regulated only in the resistant cultivar 'Florina' after aphid infestation compared to the susceptible cultivar 'Topaz' and /or mechanically wounded or non-infested leaves. These fragments were cloned, sequenced, and the pattern of gene expression for six fragments was subsequently verified by virtual Northern blots. Sequence comparisons of these fragments to GenBank accessions revealed homologies to already known genes, most of them isolated from Arabidopsis thaliana L. Among them, a putative RNase-L-inhibitor-like protein, a pectinacetylesterase, an inositol-phosphatase-like protein, a precursor of the large chain of the ribulose-1,5-biphosphate-carboxylase, and defence-related genes such as a vacuolar H(+)-ATPase subunit-like protein and an ADP-ribosylating enzyme were identified. The results are discussed in relation to a putative role of these genes in conferring aphid resistance in apple trees.

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Over‐expressed rice ADP‐ribosylation factor 1 (RARF1) induces pathogenesis‐related genes and pathogen resistance in tobacco plants

Cited by 36 publications

References 48 publications

High‐throughput in planta expression screening identifies an ADP‐ribosylation factor (ARF1) involved in non‐host resistance and R gene‐mediated resistance

High‐throughput in planta expression screening identifies an ADP‐ribosylation factor (ARF1) involved in non‐host resistance and R gene‐mediated resistance

Attenuation of the hypersensitive response by an ATPase associated with various cellular activities (AAA) protein through suppression of a small GTPase, ADP ribosylation factor, in tobacco plants

Molecular interactions between rosy apple aphids, Dysaphis plantaginea, and resistant and susceptible cultivars of its primary host Malus domestica

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