Over-expression of KdSOC1 gene affected plantlet morphogenesis in Kalanchoe daigremontiana

Abstract

Kalanchoe daigremontiana reproduces asexually by producing plantlets along the leaf margin. The aim of this study was to identify the function of the SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 gene in Kalanchoe daigremontiana (KdSOC1) during plantlet morphogenesis. In this study, KdSOC1 gene expression was detected at stem cell niche during in vitro somatic embryogenesis and plantlet morphogenesis. Disrupting endogenous auxin transportation suppressed the KdSOC1 gene response. Knockdown of the KdSOC1 gene caused a defect in cotyledon formation during the early heart stage of somatic embryogenesis. Over-expression (OE) of the KdSOC1 gene resulted in asymmetric plantlet distribution, a reduced number of plantlets, thicker leaves, and thicker vascular fibers. Higher KdPIN1 gene expression and auxin content were found in OE plant compared to those of wild-type plant leaves, which indicated possible KdSOC1 gene role in affecting auxin distribution and accumulation. KdSOC1 gene OE in DR5-GUS Arabidopsis reporting lines resulted in an abnormal auxin response pattern during different stages of somatic embryogenesis. In summary, the KdSOC1 gene OE might alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, which is crucial for plasticity during plantlet formation under various environmental conditions.

Expression profiling of MADS-box gene family revealed its role in vegetative development and stem ripening in S. spontaneum

“…In strawberry, FvSOC1 regulates the differentiation of axillary buds to runners, probably through the activation of gibberellin biosynthetic genes, which plays a central role in the photoperiodic control of both generative and vegetative growth 66 . Overexpression of KdSOC1 gene alter auxin distribution and accumulation along leaf margin to initiate plantlet formation and distribution, crucial for plasticity during plantlet formation under various environmental conditions 67 .…”

The Bacillus cereus Strain EC9 Primes the Plant Immune System for Superior Biocontrol of Fusarium oxysporum

“…The gene-specific primer pairs KalPR1-3 (forward: 5′-AACATCGCTATGTCCACGGG-3′ and reverse: 5′-CCCCAAGCGAGTCGAGTTAG-3′ for Kaladp0099s0143.1) and KalLOX2-1 (forward: 5′-TCGCAAAAACATTCCCAGCG-3′ and reverse: 5′-AGCGCTATCTCTGGCTTGTC-3′ for Kaladp0747s0006.1) [ 39 ] were designed using NCBI Primer Designing Tool [ 79 ]. The primer pair KdActin used for PCR normalisation was obtained for the Actin gene of Kalanchoe daigremontiana [ 80 ]. PCR optimisation and efficiency calculation was carried out using 1:10 standard curves of known DNA concentrations.…”

Induction of Somatic Embryogenesis in Plants: Different Players and Focus on WUSCHEL and WUS-RELATED HOMEOBOX (WOX) Transcription Factors

“…In particular, somatic embryogenesis in vivo is a remarkable phenomenon, but, unfortunately, it has not been studied in detail. It might be interesting to learn, in more depth, the role of WOX genes in epiphylly, since only a few genes have been investigated at present [26,[294][295][296][297]. In this regard, the recent study by Jácome-Blásquez et al [298] appeared to increase knowledge regarding the genes involved in the ectopic differentiation of plantlets in vivo.…”