Over-expression of miR-145 enhances the effectiveness of HSVtk gene therapy for malignant glioma

Lee, Sang-Jin; Kim, Seok-Jun; Seo, Hye-Hyun; Shin, Seung-Phil; Kim, Daehong; Park, Shin Young; Kim, Kyung‐Tae; Kim, Yun-Hee; Jeong, Jin–Sook; Kim, In-Hoo

doi:10.1016/j.canlet.2012.01.029

Cited by 48 publications

(41 citation statements)

References 36 publications

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“…Lee et al previously showed that overexpression of miR-145 can significantly inhibit migration and invasion of U87MG/U373MG cells. 46 Although negative regulation of miR-145 on OCT4 and SOX-2 has been demonstrated in glioma CD133 (+) cells, 47 the present study shows for the first time that inhibition of OCT4 and SOX-2 by curcumin may occur through miR-145 overexpression. Therefore, miRNA can International Journal of Nanomedicine 2014:9…”

mentioning

confidence: 53%

Dendrosomal curcumin nanoformulation downregulates pluripotency genes via miR-145 activation in U87MG glioblastoma cells

Mirgani¹,

Isacchi²,

Sadeghizadeh³

et al. 2014

IJN

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Glioblastoma is an invasive tumor of the central nervous system. Tumor recurrence resulting from ineffective current treatments, mainly due to the blood–brain barrier, highlights the need for innovative therapeutic alternatives. The recent availability of nanotechnology represents a novel targeted strategy in cancer therapy. Natural products have received considerable attention for cancer therapy because of general lower side effects. Curcumin is a new candidate for anticancer treatment, but its low bioavailability and water solubility represent the main disadvantages of its use. Here, curcumin was efficiently encapsulated in a nontoxic nanocarrier, termed dendrosome, to overcome these problems. Dendrosomal curcumin was prepared as 142 nm spherical structures with constant physical and chemical stability. The inhibitory role of dendrosomal curcumin on the proliferation of U87MG cells, a cellular model of glioblastoma, was evaluated by considering master genes of pluripotency and regulatory miRNA (microribonucleic acid). Methylthiazol tetrazolium assay and flow cytometry were used to detect the antiproliferative effects of dendrosomal curcumin. Annexin-V-FLUOS and caspase assay were used to quantify apoptosis. Real-time polymerase chain reaction was used to analyze the expression of OCT4 (octamer binding protein 4) gene variants ( OCT4A , OCT4B , and OCT4B1 ), SOX-2 (SRY [sex determining region Y]-box 2) , Nanog , and miR-145 . Dendrosomal curcumin efficiently suppresses U87MG cells growth with no cytotoxicity related to dendrosome. Additionally, the accumulation of cells in the SubG 1 phase was observed in a time- and dose-dependent manner as well as higher rates of apoptosis after dendrosomal curcumin treatment. Conversely, nonneoplastic cells were not affected by this formulation. Dendrosomal curcumin significantly decreased the relative expression of OCT4A , OCT4B1 , SOX-2 , and Nanog along with noticeable overexpression of miR-145 as the upstream regulator. This suggests that dendrosomal curcumin reduces the proliferation of U87MG cells through the downregulation of OCT4 (octamer binding protein 4) variants and SOX-2 (SRY [sex determining region Y]-box 2) in an miR-145 -dependent manner.

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mentioning

confidence: 53%

Dendrosomal curcumin nanoformulation downregulates pluripotency genes via miR-145 activation in U87MG glioblastoma cells

Mirgani¹,

Isacchi²,

Sadeghizadeh³

et al. 2014

IJN

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“…In vitro, ADAM22 gene expression was reduced by both miR-30b and miR-145 transfection. ADAM22 is involved in cell adhesion and migration (3), and it has been reported to be a miR-145 target (24). However, its role in adipose tissue and insulin sensitivity is currently unknown.…”

Section: Discussionmentioning

confidence: 99%

Integrative mRNA-microRNA analyses reveal novel interactions related to insulin sensitivity in human adipose tissue

Kirby

Walton

Finlin

et al. 2016

Physiological Genomics

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“…A recent study [28] demonstrated a significant correlation between miR-145 levels and tumor stage (p = 0.001) in 86 clinical samples of esophageal carcinoma and their matched normal tissues using qRT-PCR. Furthermore, Lee et al [29] suggested that high expression of miR-145 could induce downregulation of metastasis-related genes, such as ADAM22, SPOCK3, PLAUR, SLC7A5 and FASCN1, and significantly inhibit both the migration and invasion of U87MG/U373MG glioma cells. In our study, we found that there was no significant difference between low expression of miR-145 and clinicopathologic factors.…”

Section: Discussionmentioning

confidence: 99%

Downregulation of miR-145 Expression in Oral Squamous Cell Carcinomas and Its Clinical Significance

Gao

Ren

Chang³

et al. 2013

Oncol Res Treat

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Background: MicroRNAs have been reported to play roles as oncogenes or tumor suppressor genes in human cancers. However, the expression levels of miR-145 in oral squamous cell carcinoma (OSCC) are unclear. The purpose of this study was to investigate the status of miR-145 expression in OSCC and determine its clinical significance. Patients and Methods: We examined miR-145 levels in 62 OSCC tissue samples and cell lines by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The relationship between miR-145 expression and clinicopathologic factors of OSCC patients was analyzed. Results: The proportion of miR-145 low expression was 82.26% (51/62) among the 62 OSCC patients, and expression levels of miR-145 in OSCC tissue samples and cell lines were significantly lower than in non-tumor controls. miR-145 expression levels were not significantly associated with age (p = 0.607), sex (p = 0.213), location (p = 0.952), histology (p = 0.603), pT stage (p = 0.305), pTNM stage (p = 0.471), and lymphatic metastasis (p = 1.000). Conclusion: miR-145 may be involved in the early tumorigenesis of oral squamous cells, and might be a potential biomarker in the early diagnosis of OSCC.

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Over-expression of miR-145 enhances the effectiveness of HSVtk gene therapy for malignant glioma

Cited by 48 publications

References 36 publications

Dendrosomal curcumin nanoformulation downregulates pluripotency genes via miR-145 activation in U87MG glioblastoma cells

Dendrosomal curcumin nanoformulation downregulates pluripotency genes via miR-145 activation in U87MG glioblastoma cells

Integrative mRNA-microRNA analyses reveal novel interactions related to insulin sensitivity in human adipose tissue

Downregulation of miR-145 Expression in Oral Squamous Cell Carcinomas and Its Clinical Significance

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