Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures

El-Banna, Antar; Hajirezaei, Mohammad‐Reza; Wissing, Joseph; Ali, Zahid; Vaas, Lea A. I.; Heine-Dobbernack, Elke; Jacobsen, Hans‐Jörg; Schumacher, Heinz Martin; Kiesecker, Heiko

doi:10.1016/j.jbiotec.2010.09.934

Cited by 39 publications

(33 citation statements)

References 54 publications

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“…But somaclonal variation events are most likely to occur during the transformation process (Filipecki and Malepszy 2006) rather than during maintenance under normal conditions. The present study was performed with a transgenic cell line which showed consistently altered characteristics such as osmotic and salt tolerance as well as proline and glutathion metabolism (El-Banna et al 2010). With potato and peanut cell cultures it was shown that complicated and long-lasting selection procedures can produce a comparable range in tolerances to osmotic and/or salt stresses, given that the selection procedure favours beneficial somaclonal variations (Sabbah and Tal 1990;Ochatt et al 1999;Jain et al 2006).…”

Section: Discussionmentioning

confidence: 97%

“…Désirée (WT, DSMZ No. PC-1182) and a transgenic line homologously overexpressing pr-10a (GMO) were subcultured and harvested as previously described (El-Banna et al 2010). For all experiments, suspension cells were taken from the logarithmic growth phase, 3 days after the last subculturing.…”

Section: Plant Materials and Cultivationmentioning

confidence: 99%

“…We recently showed that homologous overexpression of pr-10a in a cell line of S. tuberosum cv. Désirée resulted in increased survival under salt and osmotic challenge compared to the wild-type cells (El-Banna et al 2010). The concentrations of proline and oxidized glutathione, regarded as indicators for salt and/or osmotic tolerance (Ashraf and Harris 2004), were increased (El-Banna et al 2010).…”

Section: Introductionmentioning

confidence: 97%

“…Désirée resulted in increased survival under salt and osmotic challenge compared to the wild-type cells (El-Banna et al 2010). The concentrations of proline and oxidized glutathione, regarded as indicators for salt and/or osmotic tolerance (Ashraf and Harris 2004), were increased (El-Banna et al 2010). In the present study, we modify sorbitol-and DMSO-based pretreatments in terms of sorbitol concentration and treatment duration and determine the resulting pr-10a expression patterns in both wild-type and transgenic cells.…”

Section: Introductionmentioning

confidence: 97%

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Impact of pr-10a overexpression on the cryopreservation success of Solanum tuberosum suspension cultures

et al. 2012

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Although many genes are supposed to be a part of plant cell tolerance mechanisms against osmotic or salt stress, their influence on tolerance towards stress during cryopreservation procedures has rarely been investigated. For instance, the overexpression of the pathogenesis-related gene 10a (pr-10a) leads to improved osmotic tolerance in a transgenic cell culture of Solanum tuberosum cv. Désirée. In this study, a cryopreservation method, consisting of osmotic pretreatment, cryoprotection with DMSO and controlled-rate freezing, was used to characterize the relation between cryopreservation success and pr-10a expression in suspension cultures of S. tuberosum wild-type cells and cells overexpressing pathogenesis-related protein 10a (Pr-10a). By varying the sorbitol concentration, thus modifying the strength of the osmotic stress during the pretreatment phase, it can be shown that the wild type can successfully be cryopreserved only in a relatively narrow range of sorbitol concentrations, while the pr-10a overexpression leads to an enhanced cryopreservation success over the whole range of applied sorbitol concentrations. Together with transcription data we show that the pr-10a overexpression causes an enhanced osmotic tolerance, which in turn leads to enhanced cryopreservability, but also indicates a role of pr-10a in signal transduction. An increased cryopreservability of the transgenic cell line occurs for pretreatments longer than 24 h. Since both genotypes, characterized by distinct baseline levels of expression, exhibited similar patterns of expression induction, the induction of pr-10a appears to be a key step in the stress signal transduction of plant cells under osmotic stress.

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Section: Discussionmentioning

confidence: 97%

Section: Plant Materials and Cultivationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Impact of pr-10a overexpression on the cryopreservation success of Solanum tuberosum suspension cultures

et al. 2012

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“…Characterization of PR-10 proteins and development of transgenic plants overexpressing PR-10 proteins is important step in this direction. Table 1 lists the PR-10 genes which have been used to develop transgenic plants in different crop species [95][96][97][98][99][100][101][102][103][104][105][106][107][108][109][110]. Multi-location field trials of transgenic plants expressing PR-10 will likely be next step for further evaluation.…”

Section: Pr-10 Proteins: a Resource For Crop Improvementmentioning

confidence: 99%

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

Jain¹

2015

APAR

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One of the most represented group of Pathogenesis Related (PR) genes are those of the PR-10 class. PR-10 proteins are members of multi genic family, and they often occur in clusters at specific loci following gene duplication and amplification events. To date, large number of PR-10 genes have been cloned and characterized in different species in response to abiotic and biotic stress. This review is focused on recent studies that have described the role, distribution and structure of PR-10 genes in plant genomes. Recent findings have provided insights into the functional roles of PR-10 proteins as ribonuclease, as cytokinin-specific binding proteins, a mammalian lipid transport and plant abscisic acid (ABA) receptor proteins, or as enzyme, (S)-norco claurine synthase. PR-10 proteins are differentially expressed in the presence of different signaling molecules, biotic stresses such as fungal, viral and bacterial pathogens and a number of abiotic stresses. The possibility to use this knowledge for genetic improvement of plant resistance to pathogens through classical breeding approach or transgenic technology is discussed.

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Potato: Improving Crop Productivity and Abiotic Stress Tolerance

Wang‐Pruski

Schofield

2012

Improving Crop Resistance to Abiotic Stress

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Over-expression of PR-10a leads to increased salt and osmotic tolerance in potato cell cultures

Cited by 39 publications

References 54 publications

Impact of pr-10a overexpression on the cryopreservation success of Solanum tuberosum suspension cultures

Impact of pr-10a overexpression on the cryopreservation success of Solanum tuberosum suspension cultures

The Pathogenesis Related Class 10 proteins in Plant Defense against Biotic and Abiotic Stresses

Potato: Improving Crop Productivity and Abiotic Stress Tolerance

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