OVER-EXPRESSION OF PROLINE-LINKED ANTIOXIDANT PATHWAY AND MODULATION OF PHENOLIC METABOLITES IN LONG LIFE SPAN CLONAL LINE OF<i>ORIGANUM VULGARE</i>IN RESPONSE TO ULTRAVIOLET RADIATION

Kwon, Young-In; Apostolidis, Emmanouil; Kim, Young‐Cheul; Shetty, Kalidas

doi:10.1111/j.1745-4514.2009.00243.x

Cited by 10 publications

(15 citation statements)

References 42 publications

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“…The extract samples were eluted out in an isocratic manner with a mobile phase consisting of 20 mM potassium phosphate (pH 2.5 by phosphoric acid) at a flow rate of 1 mL min −1 and detected at 210 nm. L-Proline (Sigma chemicals, St. Louis, MO) dissolved in the 20 mM potassium phosphate solution was used to calibrate the standard curve (Kwon et al 2009). The amount of proline in the sample was reported as mg of proline per milliliter and converted to mg g −1 FW.…”

Section: Hplc Analysis Of Prolinementioning

confidence: 99%

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

et al. 2016

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Plants defense responses to abiotic stresses, including salinity stress, involve stimulation of defense related pathways such as biosynthesis of secondary metabolites and induction of endogenous antioxidant enzyme responses. In the present study, a single seed origin clonal line of Swertia chirayita inoculated with Lactobacillus plantarum (LP) was grown under different salinity levels. Control had no LP inoculation. S. chirayita inoculated with LP showed higher accumulation of proline, low proline dehydrogenase activity, up-regulation of pentose phosphate pathway, down-regulation of succinate dehydrogenase activity (Krebs cycle) and low total phenolic content with increased salt concentrations. In comparison, S. chirayita without LP adopted a different biochemical mechanism to counter salt stress (NaCl) by up-regulating both pentose phosphate pathway and Krebs cycle along with stimulation of phenolic biosynthesis. Guaiacol peroxidase (GPX) activity increased with and without LP treatment in response to increasing concentrations of salt. These results indicate that S. chirayita inoculated with LP exhibits a greater salinity stress tolerance than S. chirayita without LP by adopting a more energy efficient defense responses and potentially efficiently partitioning carbon flux between primary and secondary metabolism to counter salt induced oxidative stress.

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Section: Hplc Analysis Of Prolinementioning

confidence: 99%

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

et al. 2016

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“…After initial shoot growth, O-17 line grew hardier and elongated lignified stems with less lateral branches that reduced the overall vigor and leaves required for comparative analysis with O-3. This O-17 line is a long life span line with slower overall growth and higher phenolic content (Kwon et al 2009). …”

Section: Screening and Visual Observationmentioning

confidence: 99%

“…After 1 month of growth, the leaf tissues of O-3 lines were collected for biochemical analysis. Due to the slower growth of O-17 lines, we were unable to analyze this line for biochemical activities after 30 days, but the basis of long life span has been well documented based on previous studies (Kwon et al 2009) and was the reason for choice to undertake this initial study.…”

Section: Shoot Culture Of Oregano and Nitrate Treatmentmentioning

confidence: 99%

“…Specifically, in our strategy, we have used novel tissue culture-based micropropagation technologies developed previously in our laboratory (Zheng et al 2001;Kwon et al 2009) to advance efficient nitrogen pollution removal in cold latitudes. In this approach, we have the following innovations: (a) screening right combinations of robust clonal phenotypes of plant species for nitrate removal in cold latitudes; (b) improving robustness of plant clonal system for nitrate removal using microbial interactions based on previous technologies developed in our laboratory (Strycharz and Shetty 2002); (c) integrating plant clonal system combination in greenhouse microenvironment to enhance localized nitrate removal in growing systems.…”

Section: Introductionmentioning

confidence: 99%

“…In this initial conceptual study, to evaluate the potential of oregano clonal system, we report the regulation of nitrate assimilation linked to control by pentose phosphate pathway in oregano clonal lines that also has implications for antioxidant response pathways linked to phenolic metabolites. (Eguchi et al 1996;Shetty et al 1995;Kwon et al 2009 …”

Section: Introductionmentioning

confidence: 99%

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Initial screening studies on potential of high phenolic-linked plant clonal systems for nitrate removal in cold latitudes

Lin

You

et al. 2010

J Soils Sediments

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Purpose Nitrate removal is a major challenge in drinking water systems and is more acute in colder latitudes due to low metabolic conversion rates of biological systems. To achieve rapid nitrate removal, designed plant systems in greenhouse microenvironments has potential. In such localized controlled microenvironments, higher temperatures can be managed for plant growth in an appropriate growth medium through which nitrate-contaminated water is fed for denitrification. Materials and methods In the overall strategy to develop effective plant systems for controlled environment removal of nitrate pollution, we have developed plant tissue culture technologies to isolate cold tolerant plant species that can be grown in microenvironments. The use of novel tissue culture technologies allowed isolation of plant clonal lines of single seed phenotype origin that can be screened for cold tolerance and nitrate removal in aquatic systems. Such clonal lines were evaluated for nitrate tolerance in the range of 0-50 mM. Further, selected clonal lines were evaluated for nitrate reductaselinked phenolic-linked antioxidant and pentose phosphate pathways stimulation by measuring specific enzyme activity in the range of 0-10 mM. Results and discussion Such single seed plant clonal isolations were evaluated for nitrate removal in the range of 0-10 mM in laboratory systems. One group of plant species that hold promise for use in controlled greenhouse environments are species of the family Lamiaceae such as mints and oregano. Strategy for specific clonal screening and use in cold latitude plant-based and greenhouse systems for temperature control in the winter have been proposed. These enhanced nitrate removing clonal lines can use pentose phosphate pathway stimulation to optimize nitrate removal by likely providing the needs of NADPH that are also needed for phenolic antioxidant synthesis and stimulation of associated antioxidant enzyme responses. Conclusions High nitrate pollution removal clonal systems can be screened, and such clonal lines potentially use the pentose phosphate pathway and associated phenolic-linked antioxidant pathways to manage nitrate uptake through nitrate reductase. Such clonal systems have potential to be integrated into greenhouse systems to remove nitrate pollution in water and concurrently have the ability to grow value-added plants for diverse applications such as food herbs and medicinal use.

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Exploring plant tissue culture to improve the production of phenolic compounds: A review

Días

Sousa

Alves

et al. 2016

Industrial Crops and Products

239

162

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Plant tissue and organ culture has been extensively used from the beginning of the XX century for the study and comprehension of some primary biological mechanisms such as morphogenesis. However, with the increasing demand of the market for novel products derived from plants, in vitro culture became a reliable technique for the mass production of plant material. Moreover, the potential to use this technique for the production of some bioactive compounds, such as phenolic compounds, is immense since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This work intends to make a brief historical review of in vitro culture, highlighting its use for the production of bioactive compounds. Also, emphasizes the importance of phenolic compounds for the consumer as well reviews the metabolic pathways involved in its production in plant cells.Furthermore, it was carried out a comprehensive study on the work developed for the production of plant phenolic compounds in in vitro cultures, as well as on the type of elicitors used to increase of the same production; also a brief highlighting of the phenolic compounds which serve as elicitors. There are numerous reports directed to the production of phenolic extracts in in vitro plant cultures, however there is a lack in the production of individual phenolic compounds mainly due to the complexity of the biosynthetic routes and extraction procedures. Elicitation procedures are often used to increase the production of phenolics, archieving in most cases higher yields than in nonelicitated cultures. The increasing production of bioactive phenolic extracts/compounds allows for their further applicability, namely in the industry of functional foods or in pharmaceutical/medical fields.3

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OVER-EXPRESSION OF PROLINE-LINKED ANTIOXIDANT PATHWAY AND MODULATION OF PHENOLIC METABOLITES IN LONG LIFE SPAN CLONAL LINE OFORIGANUM VULGAREIN RESPONSE TO ULTRAVIOLET RADIATION

Cited by 10 publications

References 42 publications

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

Improving salinity resilience inSwertia chirayitaclonal line withLactobacillus plantarum

Initial screening studies on potential of high phenolic-linked plant clonal systems for nitrate removal in cold latitudes

Exploring plant tissue culture to improve the production of phenolic compounds: A review

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