Over-expression of StLCYb increases β-carotene accumulation in potato tubers

Song, Xin; Zhu, Wenjiao; Tang, Ruimin; Cai, Jinghui; Chen, Min; Yang, Qing

doi:10.1007/s11816-016-0390-y

Cited by 27 publications

(10 citation statements)

References 27 publications

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“…This result was similar to previous results in other carotenoid synthetic genes. For example, potato tubers overexpressing StLCYb appeared yellow; in contrast, the control plant was light-white [28]. Canola seeds (Brassica napus) overexpressing crtB (a bacterial phytoene synthase gene) were visibly orange [29].…”

Section: Discussionmentioning

confidence: 99%

Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Jian

Zhang

Wang

et al. 2021

Genes

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Chinese kale (Brassica oleracea var. alboglabra) is rich in carotenoids, and neoxanthin is one of the most important carotenoids in Chinese kale. In this study, the function of the neoxanthin synthase gene (BoaNXS) in Chinese kale was investigated. BoaNXS, which had a 699-bp coding sequence, was cloned from the white flower cultivar of Chinese kale and was expressed in all developmental stages and organs of Chinese kale; its expression was highest in young seeds. The subcellular localization indicated that BoaNXS was localized in the chloroplast. BoaNXS-overexpressed plants were obtained via Agrobacterium-mediated transient overexpression methodology, and the gene overexpression efficiencies ranged from 2.10- to 4.24-fold. The color in the leaves of BoaNXS-overexpressed plants changed from green to yellow-green; the content of total and individual carotenoids, such as neoxanthin, violaxanthin, and lutein, was significantly increased, and the expression levels of most carotenoid biosynthetic genes were notably increased. These findings indicated that BoaNXS is of vital importance in carotenoid biosynthesis in Chinese kale and could be used as a candidate gene for enriching the carotenoid accumulation and color of Chinese kale and other Brassica vegetables.

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Section: Discussionmentioning

confidence: 99%

Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Jian

Zhang

Wang

et al. 2021

Genes

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“…Silencing of the two β-carotene hydroxylases in sweet potato preventing the conversion of β-carotene to zeaxanthin resulted in increase of up to 38-fold in β-carotene level and up to 4.5-fold in total carotenoids content of the transgenic tubers [30]. Overexpression of LCY-b gene in potato however was only able to raise the β-carotene content level by 1.9-fold [42]. Multigene engineering used in transgenic potatoes expressing three genes, encoding crtB , crtI and crtY from Erwinia (Fig.…”

Section: Engineering Carotenoids In Staple Cropsmentioning

confidence: 98%

Elevated carotenoids in staple crops: The biosynthesis, challenges and measures for target delivery

Badejo

2018

Journal of Genetic Engineering and Biotechnology

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Poverty eradication and global food security are among the targets of world leaders, most especially combating the scourge of hidden hunger. Provitamin A carotenoids cannot be synthesized de novo by human and so it must be taken as part of the diet. The deficiency of which is causing almost 6000 sights to be lost daily in most developing countries because of the monotonous starchy diets lacking substantial amount of carotenoid. Conventional breeding as well as genetic engineering have been used to increase the level of carotenoid in many staples including rice, potato, maize and cassava. While products from genetic engineering are still been subjected to strict regulatory measures preventing the delivery of the products to target consumers, some of the products from conventional breeding are already on the table of consumers. Interestingly, both technologies are crucial to tackling micronutrient deficiencies. This review discusses the role of carotenoid in human, the biosynthesis in plant and some of the staple crops that have been modified for increased carotenoid. Some measures expected of the leaders of the countries in need of these products for safe delivery to the target population after two decades is also highlighted.

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“…In potato tuber, provitamin A (carotenoid forms) have been increased by incorporating PSY gene ( 127 ) and by simultaneous incorporation of three genes: PSY , phytoene desaturase, and lycopene β-cyclase ( 128 ). Beta-carotene content in tubers has been also enhanced by using RNAi to silence the beta-carotene hydroxylase gene (bch), which converts beta-carotene to zeaxanthin ( 129 ) and by regulation of beta-carotene synthesis through expression of lycopene β-cyclase [ StLCYb ( 130 )]. In another experiment, it has been observed that incorporation of Or gene from orange cauliflower mutant leads to increase in carotenoids along with three additional metabolite intermediates phytoene, phytofluene, and z-carotene ( 131 ).…”

Section: Transgenic Vegetablesmentioning

confidence: 99%

Biofortified Crops Generated by Breeding, Agronomy, and Transgenic Approaches Are Improving Lives of Millions of People around the World

et al. 2018

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Biofortification is an upcoming, promising, cost-effective, and sustainable technique of delivering micronutrients to a population that has limited access to diverse diets and other micronutrient interventions. Unfortunately, major food crops are poor sources of micronutrients required for normal human growth. The manuscript deals in all aspects of crop biofortification which includes—breeding, agronomy, and genetic modification. It tries to summarize all the biofortification research that has been conducted on different crops. Success stories of biofortification include lysine and tryptophan rich quality protein maize (World food prize 2000), Vitamin A rich orange sweet potato (World food prize 2016); generated by crop breeding, oleic acid, and stearidonic acid soybean enrichment; through genetic transformation and selenium, iodine, and zinc supplementation. The biofortified food crops, especially cereals, legumes, vegetables, and fruits, are providing sufficient levels of micronutrients to targeted populations. Although a greater emphasis is being laid on transgenic research, the success rate and acceptability of breeding is much higher. Besides the challenges biofortified crops hold a bright future to address the malnutrition challenge.

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Over-expression of StLCYb increases β-carotene accumulation in potato tubers

Cited by 27 publications

References 27 publications

Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Characterization of the Role of the Neoxanthin Synthase Gene BoaNXS in Carotenoid Biosynthesis in Chinese Kale

Elevated carotenoids in staple crops: The biosynthesis, challenges and measures for target delivery

Biofortified Crops Generated by Breeding, Agronomy, and Transgenic Approaches Are Improving Lives of Millions of People around the World

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