Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance

Lim, Chan Ju; Hwang, Jin-Woo; Chen, Huan; Hong, Joon Ki; Yang, Kiyull; Choi, Man Soo; Lee, Kyun Oh; Chung, Woo Sik; Lee, Sang Yeol; Lim, Chae Oh

doi:10.1016/j.bbrc.2007.08.007

Cited by 112 publications

(69 citation statements)

References 35 publications

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“…In addition, CCA1 peaks also are observed at the DREB2C homologs C-repeat binding factor (CBF)1, -2, and -3 as previously reported, and, in fact, CBF1 also interacts with the CCA1 promoter (50). Many of the downstream targets of CBFs/DREB2C, including cold-responsive protein 6.6 (COR6.6/KIN2), COR27, and COR15B, are also occupied by CCA1, further highlighting the complexity and crosstalk between clock-controlled pathways (47).…”

Section: Discussionsupporting

confidence: 69%

“…Among others, we observe misregulation of RING MEMBRANE-ANCHOR 1 (RMA1), an uncharacterized protein with possible E3 ubiquitin ligase activity, and DREB2C, a member of the DREB subfamily A-2 of the ERF/ AP2 transcription factor family implicated in drought stress responses (Fig. 4 B-E) (46)(47)(48)(49). Interestingly, in addition to DREB2C, 39 other transcription factors identified as CCA1 targets in this study recently have been shown to interact with the CCA1 promoter in a yeast one-hybrid assay (Dataset S3) (50).…”

Section: Countmentioning

confidence: 97%

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Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Nagel

Doherty

Pruneda‐Paz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

234

279

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The circadian clock in Arabidopsis exerts a critical role in timing multiple biological processes and stress responses through the regulation of up to 80% of the transcriptome. As a key component of the clock, the Myb-like transcription factor CIRCADIAN CLOCK ASSOCIATED1 (CCA1) is able to initiate and set the phase of clockcontrolled rhythms and has been shown to regulate gene expression by binding directly to the evening element (EE) motif found in target gene promoters. However, the precise molecular mechanisms underlying clock regulation of the rhythmic transcriptome, specifically how clock components connect to clock output pathways, is poorly understood. In this study, using ChIP followed by deep sequencing of CCA1 in constant light (LL) and diel (LD) conditions, more than 1,000 genomic regions occupied by CCA1 were identified. CCA1 targets are enriched for a myriad of biological processes and stress responses, providing direct links to clock-controlled pathways and suggesting that CCA1 plays an important role in regulating a large subset of the rhythmic transcriptome. Although many of these target genes are evening expressed and contain the EE motif, a significant subset is morning phased and enriched for previously unrecognized motifs associated with CCA1 function. Furthermore, this work revealed several CCA1 targets that do not cycle in either LL or LD conditions. Together, our results emphasize an expanded role for the clock in regulating a diverse category of genes and key pathways in Arabidopsis and provide a comprehensive resource for future functional studies.genome-wide | circadian clock | clock-controlled outputs | transcriptional regulation I n anticipation of the daily changes in light and temperature, the plant's internal circadian clock regulates a large portion of the transcriptome, nearly 80% in rice, poplar, and Arabidopsis (1, 2). The clock provides temporal coordination of multiple biological functions to ensure optimal efficiency. For example, before dawn, photosynthetic transcripts accumulate in anticipation of the expected sunlight. Even when shifted to constant light (LL) and temperature, in the absence of day/night cues, transcripts associated with photosynthesis are still up-regulated before the subjective day (1-4). Not only are light-and energyrelated activities restricted to particular times, but other aspects of plant growth, including water use, hormone activity, UVB response, and low-temperature response, show this gating effect (5-9). This temporal partitioning of biological responses ultimately provides an optimal integration of the plant's organismal functions with the environment.The circadian clock in plants involves a posttranscriptional component and a well-studied transcriptional-translation feedback regulation between the Myb-like transcription factors CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) and a member of the PSEUDO RESPONSE REGULATOR (PRR) family, TIMING OF CAB2 EXPRESSION 1 (TOC1) (10-16). Together these three components contribute t...

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Section: Discussionsupporting

confidence: 69%

Section: Countmentioning

confidence: 97%

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Nagel

Doherty

Pruneda‐Paz

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

234

279

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“…145-528 or GST-HsfA3 was expressed in E. coli BL21 (DE3) pLysE and purified using affinity chromatography as described by Lim et al (2007). The specificity of cis element-binding was measured by competition assays using unlabeled APX2-HSE-85 and HsfA3-DRE-80 oligonucleotides (Chen et al, 2010).…”

Section: Electrophoretic Mobility Shift Assay (Emsa)mentioning

confidence: 99%

“…Recent studies identified heat shock transcription factor A3 (HsfA3) as a highly up-regulated heatinducible gene in transgenic plants over-expressing DREB2s (Chen et al, 2010;Yoshida et al, 2008). Moreover, transient promoter reporter assays using mesophyll protoplasts demonstrated that HsfA3 expression is directly regulated by DREB2s under conditions of heat stress (Chen et al, 2010;Schramm et al, 2008) and that DREB2-overexpressing transgenic plants have increased tolerance to heat stress (Almoguera et al, 2009;Lee et al, 2010;Lim et al, 2007;Matsukura et al, 2010). Recent work established that DREB2C physically interacts with ABF2, a bZIP protein regulating abscisic acid (ABA)-responsive gene expression, and its overexpression affected ABA sensitivity .…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Hwang

Lim

Chen

et al. 2012

Molecules and Cells

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Dehydration-responsive element-binding proteins (DREBs) regulate plant responses to environmental stresses. In the current study, transcription of DREB2C, a class 2 Arabidopsis DREB, was induced by a superoxide anion propagator, methyl viologen (MV). The oxidative stress tolerance of DREB2C-overexpressing transgenic plants was significantly greater than that of wild-type plants, as measured by ion leakage and chlorophyll fluorescence under light conditions. The transcriptional activity of several ascorbate peroxidase (APX) genes as well as APX protein activity was induced in DREB2C overexpressors. Additionally, the level of H 2 O 2 in the overexpressors was lower than in wt plants under similar oxidative stress conditions. An electrophoretic mobility shift assay and transient activatorreporter assay showed that APX2 expression was regulated by heat shock factor A3 (HsfA3) and that HsfA3 is regulated at the transcriptional level by DREB2C. These results suggest that DREB2C plays an important role in promoting oxidative stress tolerance in Arabidopsis.

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“…AtCBF1, AtCBF2, and AtCBF3 (DREB1B, DREB1C, and DREB1A; respectively) are up regulated significantly and rapidly by cold stress specifically, but much less so by ABA or dehydration [12,13]. In contrast, AtCBF4 (DREB1D) and AtDREB2 genes are more specifically regulated by ABA, salinity, and dehydration but not by cold stress [12,14,15].…”

Section: Introductionmentioning

confidence: 98%

Functionality of soybean CBF/DREB1 transcription factors

Yamasaki

Randall

2016

Plant Science

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Soybean (Glycine max) is considered to be cold intolerant and is not able to significantly acclimate to cold/freezing stress. In most cold tolerant plants, the Crepeat/DRE Binding Factors (CBF/DREBs) are critical contributors to successful cold-responses; rapidly increasing following cold treatment and regulating the induction of many cold responsive genes. In soybean vegetative tissue, we found strong, transient accumulation of CBF transcripts in response to cold stress; however, the soybean transcripts of typical cold responsive genes (homologues to Arabidopsis genes such as dehydrins, ADH1, RAP2.1, and LEA14) were not significantly altered.Soybean CBFs were found to be functional, as when expressed constitutively in Arabidopsis they increased the levels of AtCOR47 and AtRD29a transcripts and increased freezing tolerance as measured by a decrease in leaf freezing damage and ion leakage. Furthermore the constitutive expression of GmDREB1A;2 and GmDREB1B;1 in Arabidopsis led to stronger up-regulation of downstream genes and more freezing tolerance than GmDREB1A;1, the gene whose transcript is the major contributor to total CBF/DREB1 transcripts in soybean. The inability for the soybean CBFs to significantly up regulate the soybean genes that contribute to cold tolerance is consistent with poor acclimation capability and the cold intolerance of soybean.

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Over-expression of the Arabidopsis DRE/CRT-binding transcription factor DREB2C enhances thermotolerance

Cited by 112 publications

References 35 publications

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Genome-wide identification of CCA1 targets uncovers an expanded clock network inArabidopsis

Overexpression of Arabidopsis Dehydration- Responsive Element-Binding Protein 2C Confers Tolerance to Oxidative Stress

Functionality of soybean CBF/DREB1 transcription factors

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