Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H2O2treatment

Ortiz-Espín, Ana; Locato, Vittoria; Camejo, Daymi; Schiermeyer, Andreas; Gara, Laura De; Sevilla, Francisca; Jiménez, Ana

doi:10.1093/aob/mcv076

Cited by 27 publications

(22 citation statements)

References 75 publications

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“…However, in yeast trx1/trx2 double mutants, an increase in glutathione content (mainly GSSG) has been reported (Muller ). This putative compensation has been also observed when the gene expression of two TRX h was shown to increase in GSH‐deficient mutants (Schnaubelt et al ) or in Tobacco BY‐2 cells over‐expressing Ps TRX o 1 presenting lower levels of GSH than non‐over‐expressing cells (Ortiz‐Espín et al , Calderón et al ). From a physiological point of view, the glutathione redox state could be more influential in explaining the effects of different stresses, in the control of gene expression and protein function (Gómez et al , Rellán‐Alvarez et al , Schnaubelt et al ).…”

Section: Discussionmentioning

confidence: 65%

“…The induction of the activity was correlated with the increase in the capacity of AOX, as well as a higher demand to regenerate the oxidized form of PRXIIF (Martí et al ), probably influencing the higher levels of H 2 O 2 found in our KO mutants under salinity. Furthermore, higher amount of TRX o 1 in Pstrxo1 over‐expressing tobacco BY‐2 cells protected them against induced oxidative stress by exogenous H 2 O 2 treatment, increasing some antioxidant enzyme activities and their viability (Ortiz‐Espín et al ), pointing TRX o 1 as a key component in the defense against oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

“…Carbonyl protein content was measured as carbonyl content according to Levine et al () using 2,4‐dinitrophenylhydrazine as described by Ortiz‐Espín et al (). The total soluble protein content was quantified using Bradford's method (Bradford ).…”

Section: Methodsmentioning

confidence: 99%

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Lack of mitochondrial thioredoxin o1 is compensated by antioxidant components under salinity in Arabidopsis thaliana plants

Calderón

Sánchez-Guerrero

Ortiz-Espín

et al. 2018

Physiologia Plantarum

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In a changing environment, plants are able to acclimate to new conditions by regulating their metabolism through the antioxidant and redox systems involved in the stress response. Here, we studied a mitochondrial thioredoxin in wild-type (WT) Arabidopis thaliana and two Attrxo1 mutant lines grown in the absence or presence of 100 mM NaCl. Compared to WT plants, no evident phenotype was observed in the mutant plants under control condition, although they had higher number of stomata, loss of water, nitric oxide and carbonyl protein contents as well as higher activity of superoxide dismutase (SOD) and catalase enzymes than WT plants. Under salinity, the mutants presented lower water loss and higher stomatal closure, H O and lipid peroxidation levels accompanied by higher enzymatic activity of catalase and the different SOD isoenzymes compared to WT plants. These inductions may collaborate in the maintenance of plant integrity and growth observed under saline conditions, possibly as a way to compensate the lack of TRXo1. We discuss the potential of TRXo1 to influence the development of the whole plant under saline conditions, which have great value for the agronomy of plants growing under unfavorable environment.

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 99%

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Lack of mitochondrial thioredoxin o1 is compensated by antioxidant components under salinity in Arabidopsis thaliana plants

Calderón

Sánchez-Guerrero

Ortiz-Espín

et al. 2018

Physiologia Plantarum

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“…RT-qPCR was performed on a 7500 Real Time PCR System (Applied Biosystems, USA) with SYBR Green Supermix (Bio-Rad, Madrid, Spain) [23]. Each reaction was performed in triplicate.…”

Section: Quantitative Real Time Pcr (Qrt-pcr)mentioning

confidence: 99%

Is Autophagy Involved in Pepper Fruit Ripening?

López-Vidal

Olmedilla

Sandalio

et al. 2020

Cells

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Autophagy is a universal self-degradation process involved in the removal and recycling of cellular constituents and organelles; however, little is known about its possible role in fruit ripening, in which the oxidation of lipids and proteins and changes in the metabolism of different cellular organelles occur. In this work, we analyzed several markers of autophagy in two critical maturation stages of pepper (Capsicum annuum L.) fruits where variations due to ripening become clearly visible. Using two commercial varieties that ripen to yellow and red fruits respectively, we studied changes in the gene expression and protein content of several autophagy (ATG) components, ATG4 activity, as well as the autophagy receptor NBR1 and the proteases LON1 and LON2. Additionally, the presence of intravacuolar vesicles was analyzed by electron microscopy. Altogether, our data reveal that autophagy plays a role in the metabolic changes which occur during ripening in the two studied varieties, suggesting that this process may be critical to acquiring final optimal quality of pepper fruits.

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“…PRXs can decrease the level of mitochondrial H 2 O 2 using reduced TRX or GSH as reductant sources, which in turn are reduced by thioredoxin reductase and GR [62]. At-PRXII F is one of six types II PRX identified in the genome of Arabidopsis and the only PRX that is targeted to the plant mitochondrion, which is essential for redox homeostasis by the decomposition of peroxides and by playing role in protecting the mitochondria during pathogen infection [62,70].…”

Section: Mitochondria-associated Ros Metabolism and Non-ros Moleculesmentioning

confidence: 99%

Effects of Salicylic Acid on the Metabolism of Mitochondrial Reactive Oxygen Species in Plants

Poór

2020

Biomolecules

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Different abiotic and biotic stresses lead to the production and accumulation of reactive oxygen species (ROS) in various cell organelles such as in mitochondria, resulting in oxidative stress, inducing defense responses or programmed cell death (PCD) in plants. In response to oxidative stress, cells activate various cytoprotective responses, enhancing the antioxidant system, increasing the activity of alternative oxidase and degrading the oxidized proteins. Oxidative stress responses are orchestrated by several phytohormones such as salicylic acid (SA). The biomolecule SA is a key regulator in mitochondria-mediated defense signaling and PCD, but the mode of its action is not known in full detail. In this review, the current knowledge on the multifaceted role of SA in mitochondrial ROS metabolism is summarized to gain a better understanding of SA-regulated processes at the subcellular level in plant defense responses.

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Over-expression of Trxo1 increases the viability of tobacco BY-2 cells under H₂O₂treatment

Cited by 27 publications

References 75 publications

Lack of mitochondrial thioredoxin o1 is compensated by antioxidant components under salinity in Arabidopsis thaliana plants

Lack of mitochondrial thioredoxin o1 is compensated by antioxidant components under salinity in Arabidopsis thaliana plants

Is Autophagy Involved in Pepper Fruit Ripening?

Effects of Salicylic Acid on the Metabolism of Mitochondrial Reactive Oxygen Species in Plants

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