Overdose fertilization induced ammonia-oxidizing archaea producing nitrous oxide in intensive vegetable fields

Duan, Pengpeng; Fan, Changhua; Zhang, Qianqian; Xiong, Zhengqin

doi:10.1016/j.scitotenv.2018.09.341

Cited by 54 publications

(38 citation statements)

References 67 publications

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“…In general, NO 2 − cannot accumulate in the soil unless NO 2 − consumption is less than NO 2 − production (Burns, Stevens, & Laughlin, 1996). NO 2 − production increased with the nitrogen input rate due to a rapid nitrification process (Duan, Fan, Zhang, & Xiong, 2019); therefore, the transitory peak of NO 2 − was observed on Day 3 after nitrogen application. Interestingly, the NO 2 − concentrations for NI treatments were lower than those for 0N, even though nitrogen was applied in these plots.…”

Section: Pls-pm Analysismentioning

confidence: 99%

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Liu

et al. 2020

European J Soil Science

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Nitrous oxide (N 2 O) is a potent greenhouse gas that is released from agricultural ecosystems where nitrogen fertilizers are poorly used and contributes to global climate warming. Large nitrogen fertilizer inputs in summer maize fields on the North China Plain (NCP) lead to large N 2 O emissions. N 2 O emissions can be mitigated using 3,4-dimethylpyrazole phosphate (DMPP), but the efficiency and microbial mechanisms of this mitigation at different nitrogen rates remain poorly understood. To evaluate the efficiency of N 2 O emission mitigation by DMPP at different nitrogen rates and to explore its mechanisms at the microbial level, we monitored the dynamic changes in mineral and N 2 O fluxes and analysed the abundance and community structure of ammonia oxidizers. We found that DMPP significantly inhibited the oxidation of ammonium (NH 4 + ) to nitrate (NO 3 − ) and thus maintained NH 4 + concentrations but decreased the peak value of NO 3 − concentrations. Total N 2 O emissions were increased by 6.5-fold, 13.2-fold and 26.7-fold in fields with 112.5 kg N ha −1 , 225 kg N ha −1 and 337.5 kg N ha −1 , respectively, compared with fields with no nitrogen applied. Nitrogen increased the abundance of ammonia-oxidizing bacteria (AOB) and decreased that of ammonia-oxidizing archaea (AOA), whereas DMPP exhibited the opposite effects. Therefore, AOB could be the dominant N 2 O emission contributors in nitrogen-treated soils. Sequencing results suggested that all AOB belong to the Nitrosospira and Nitrosomonas nitrosa groups, and all AOA fell within the Nitrososphaera group. In both AOB and AOA communities, changes in the second-largest cluster after the application of nitrogen fertilizer and DMPP were more pronounced than those in the

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Section: Pls-pm Analysismentioning

confidence: 99%

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Liu

et al. 2020

European J Soil Science

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“…However, the AOA groups were still observed to have a small contribution (10-20%) to the N 2 O emission (Wang et al ., 2016; Hink et al ., 2017; Meinhardt et al ., 2018). Moreover, in some agricultural soils, the ammonia oxidation and N 2 O production of AOA would not be suppressed by the fertilization with high ammonium (Schauss et al ., 2009; Stopnišek et al ., 2010; Lu et al ., 2015; Duan et al ., 2019). It seems that some unknown AOA species was able to tolerate high ammonia in these soils.…”

Section: Introductionmentioning

confidence: 99%

Physiological and genomic analysis of “CandidatusNitrosocosmicus agrestis”, an ammonia tolerant ammonia-oxidizing archaeon from vegetable soil

Liu

Jiang

et al. 2019

Preprint

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The presences of ammonia tolerant ammonia-oxidizing archaea (AOA) in environments are always underestimated and their adaption to complex habitats has also rarely been reported. Here we present the physiological and genomic characteristics of an ammonia tolerant soil AOA strain Candidatus Nitrosocosmicus agrestis. This strain was able to form aggregates and adhere on the surface of hydrophobic matrix. Ammonia-oxidizing activities were still observed at 200 mM NH4+ (> 1500 μM of free ammonia) and 50 mM NO2-. Urea could be used as sole energy source but exogenous organics had no significant effect on the ammonia oxidation. Besides the genes involving in ammonia oxidation, carbon fixation and urea hydrolysis, the genome also encodes a full set of genes (GTs, GHs, CEs, MOP, LPSE, etc) that responsible for polysaccharide metabolism and secretion, suggesting the potential production of extracellular polymeric substances (EPS). Moreover, a pathway connecting urea cycle, polyamines synthesis and excretion was identified in the genome, which indicates the NH4+ in cytoplasm could potentially be converted into polyamines and excreted out of cell, and then contributes to the high ammonia tolerance. Genes encoding the cytoplasmic carbonic anhydrase and putative polyamine exporter are unique in Ca. Nitrosocosmicus agrestis or the genus Ca. Nitrosocosmicus, suggesting the prevalence of ammonia tolerance in this clade. The proposed mechanism of ammonia tolerance via polyamines synthesis and export was verified by using transcriptional gene regulation and polyamines determination.IMPORTANCEAOA are ubiquitous in different environments and play a major role in nitrification. Though AOA have higher affinities for ammonia, their maximum specific cell activity and ammonia tolerance are usually much lower than AOB, resulting in low contribution to the global ammonia oxidation and N2O production. However, in some agricultural soils, the AOA activity would not be suppressed by the fertilization with high concentration of ammonium nitrogen, suggesting the presence of some ammonia tolerant species. This study provides some physiological and genomic characteristics for an ammonia tolerant soil AOA strain Ca. Nitrosocosmicus agrestis and proposes some mechanisms of this AOA adapting to a variety of environments and tolerating to high ammonia. Ammonia tolerance of AOA was always underestimated in many previous studies, physiological and genomic analyses of this AOA clade are benefit to uncover the role of AOA playing in global environmental patterns.

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“…For white clover and Italian ryegrass grown either alone or in combination on dairy farms, 50:50 urea and ammonium sulfate application in accordance with industry standards and legislation is likely to have a marked effect on soil bacterial communities related to nutrient uptake by plants in addition to overall plant biomass effects when N exceeds 180 kg of N/ha per year (Iannetta et al, 2016). Recent studies that highlight some of the bacteria effects on soil and plants in a similar N concentration range include Wepking et al (2017) and Duan et al (2019). We also expect that the bacterial community changes are associated with N cycling changes; however, because quantifying N cycling and N cycling efficiency is not as simple as assessing N required for pasture plant biomass yield (Holly et al, 2018), many of the microbiological responses occurring in these production environments are often overlooked.…”

Section: Discussionmentioning

confidence: 99%

“…The presence of legumes in dairy pastures can improve N use efficiency (van Eekeren et al, 2009;Lüscher et al, 2014), but excessive use of N fertilizer aimed at maximizing biomass for production gain is common practice in some places (Acosta-Martínez et al, 2010;Chapman et al, 2014;McDowell, 2017). The addition of high rates of N fertilizer to dairy pastures has potential to significantly reduce the effectiveness of soil microbial processes associated with efficient nutrient cycling, which may lead to overdose fertilization and downstream effects (Wepking et al, 2017;Duan et al, 2019). This may occur where excessive N accumulates and forms hotspots (e.g., urine patches), with total N entering ecosystems of up to 2,000 kg/ha per year (Cameron et al, 2013;Chadwick et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Dairy soil bacterial responses to nitrogen application in simulated Italian ryegrass and white clover pasture

Svatos

Abbott

2019

Journal of Dairy Science

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Through clearing and use of fertilizer and legumes, areas of southwestern Australia's unique coastal sand plains can support relatively low-cost dairies. However, the ancient, highly weathered nature of the soils in this region makes the dairies susceptible to a range of threats, including nutrient leaching and erosion. Despite this, Western Australian dairy cows typically produce up to 5,500 L of milk per head annually supported by inorganic nitrogen (N) fertilizer (commonly 50:50 urea and ammonium sulfate) at rates up to <320 kg of N/ha per year. Where hotspots exist (up to 2,000 kg of N/ha per year), total N exceeds pasture requirements. We investigated plant and soil bacteria responses to N fertilizer rates consistent with Australian legislated production practices on dairy farms for pure and mixed swards of white clover (Trifolium repens) and Italian ryegrass (Lolium multiflorum) in a long-term pasture experiment in controlled glasshouse conditions. Although the soil bacterial community structure at phylum level was similar for white clover and Italian ryegrass, relative abundances of specific subgroups of bacteria differed among plant species according to the N fertilizer regimen. Marked increases in relative abundance of some bacterial phyla and subphyla indicated potential inhibition of N cycling, especially for N hotspots in soil. Ammonium concentration in soil was less correlated with dominance of some N-cycling bacterial phyla than was nitrate concentration. Changes in bacterial community structure related to altered nutrient cycling highlight the potential for considering this area of research in policy assessment frameworks related to nutrient loads in dairy soils, especially for N.

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Overdose fertilization induced ammonia-oxidizing archaea producing nitrous oxide in intensive vegetable fields

Cited by 54 publications

References 67 publications

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Physiological and genomic analysis of “CandidatusNitrosocosmicus agrestis”, an ammonia tolerant ammonia-oxidizing archaeon from vegetable soil

Dairy soil bacterial responses to nitrogen application in simulated Italian ryegrass and white clover pasture

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Overdose fertilization induced ammonia-oxidizing archaea producing nitrous oxide in intensive vegetable fields

Cited by 54 publications

References 67 publications

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N2O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N2O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Physiological and genomic analysis of “CandidatusNitrosocosmicus agrestis”, an ammonia tolerant ammonia-oxidizing archaeon from vegetable soil

Dairy soil bacterial responses to nitrogen application in simulated Italian ryegrass and white clover pasture

Contact Info

Product

Resources

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Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain

Nitrification inhibitor 3,4‐dimethylpyrazole phosphate (DMPP) reduces N₂O emissions by altering the soil microbial community in a wheat–maize rotation on the North China Plain