Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera

Wu, Shuwen; Yang, Yihua; Yuan, Guangjie; Campbell, Peter M.; Teese, Mark G.; Russell, Robyn J.; Oakeshott, John G.; Wu, Yingliang

doi:10.1016/j.ibmb.2010.09.007

Cited by 92 publications

(95 citation statements)

References 46 publications

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“…Metabolic resistance occurs via increases in either protein levels or the activity of detoxification enzymes that resist insecticides. For example, esterases (Wu et al 2011), glutathione S-transferases (Lumjuan et al 2011), and cytochrome P450 (CYP or P450; Edi et al 2014) have all been demonstrated to be associated with metabolic resistance. In addition, insecticides induce physiological changes in mosquitoes such as a thickening of the epidermis, which subsequently lowers their permeability to insecticides (Wood et al 2010).…”

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confidence: 99%

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Lei

Hong

et al. 2015

J Med Entomol

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The mosquito, Culex pipiens pallens (L.), is an important vector of encephalitis and filariasis in northern China. The control of these mosquitoes occurs primarily via the use of pyrethroid insecticides, such as deltamethrin. The widespread and improper application of pyrethroid has resulted in the evolution of pyrethroid resistance amongst many mosquito populations, including Cx. pipiens pallens. Previous studies using high-throughput transcriptome sequencing have identified that the venom allergen 5 gene is differentially expressed between deltamethrin-susceptible and deltamethrin-resistant Cx. pipiens pallens. In this study, quantitative real-time polymerase chain reaction analyses revealed that venom allergen 5 was significantly overexpressed in adult females of both deltamethrin-resistant laboratory populations and two field populations. The transcriptional level of venom allergen 5 in the laboratory populations was elevated as the levels of deltamethrin resistance increased. Full-length cDNAs of the venom allergen 5 gene were cloned from Cx. pipiens pallens, and contained an open reading frame of 765 bp, encoding a protein with 254 amino acids. The deduced amino acid sequence shared 100% identity with the ortholog in Culex quinquefasciatus Say. The overexpression of venom allergen 5 decreased the susceptibility of mosquito cells to deltamethrin, while knockdown of this gene by RNAi increased the susceptibility of mosquitoes to deltamethrin. This study provides the first evidence of the association between the venom allergen 5 gene and deltamethrin resistance in mosquitoes.

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confidence: 99%

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Lei

Hong

et al. 2015

J Med Entomol

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“…The esterases have great versatility and broad substrate specificity and are generally involved in metabolism of organophosphorus insecticides. Esterases involved in detoxification of insecticides have often been reported to be present in enhanced quantities in resistant insect populations (Nehare et al 2010;Oppenoorth 1985;Soderlund and Bloomquist 1990;Wu et al 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Such a failure often occurs due to a change in the susceptibility levels of the pest species to synthetic insecticides. Susceptibility degree changes due to metabolic detoxification of the insecticides through higher activity of some detoxifying enzymes under the stress of different management practices (Jensen 1998(Jensen , 2000Komagata et al 2010;Maymo et al 2002;Wu et al 2011). Generally three principal enzymesgeneral esterases (GEs), glutathione-s-transferases (GSTs) and cytochrome P450 mediated monooxygenases (CYPs) -are involved in the process of metabolic detoxification of insecticides.…”

Section: Introductionmentioning

confidence: 99%

Seasonal incidence and enzyme-based susceptibility to synthetic insecticides in two upcoming sucking insect pests of tea

2011

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Climate change, deforestation and over reliance on chemical pesticides during the last five decades are presumed to have had a significant impact on the incidence and abundance of agricultural pests. The thrips (Scirtothrips dorsalis Hood) and the greenfly (Empoasca flavescens Fabricius), which were previously considered as minor or occasionally as serious pests in localized areas of tea plantations, are now established as regular and at times major pests in tea plantations of North Bengal spread over the sub Himalayan slopes and the adjoining plains of Terai and the Dooars. Tea-planters from different pockets of North Bengal have been repeatedly reporting control failures of these pests with the use of insecticides in recommended dilutions. A study of their seasonal incidence along with activity of detoxifying enzymes was considered. The trend of a recent population study revealed that E. flavescens and S. dorsalis were abundant throughout the year, with peaks during April to June. Abiotic factors such as temperature, rainfall, sunshine hours and relative humidity (afternoon) influenced the population abundance. Insecticideexposed populations showed higher levels of activity of the detoxifying enzymes than those collected from organic plantations and the laboratory-reared populations that had not been exposed to insecticides. Detoxifying enzymes in insecticide-exposed E. flavescens collected from Terai and the Dooars as compared with laboratory control specimens showed 3.0-5.2 and 3.0-9.7-fold increases in general esterases (GEs), 1.5-4.8 and 3.6-5.3-fold increases in cytochrome P450s (CYPs) and 1.2-3.5 and 1.5-2.5-fold increases in glutathione-s-transferases (GSTs), respectively. Similarly, S. dorsalis collected from Terai and the Dooars showed 2.0-6.0 and 2.3-5.6-fold increases in GEs, 1.5-2.3 and 1.6-2.4-fold increases in CYPs and 2.6-3.7 and 2.3-3.6-fold increases for GSTs, respectively. Bands of isozymes of esterase I-VI and I-V were found to be expressed in insecticideexposed specimens whereas a negligible expression was evident for the bands in unexposed E. flavescens and S. dorsalis of organic plantations and laboratory-reared origins, respectively. The data obtained in the present study would be useful in developing the integrated resistance management strategies leading to effective management of the said pests.

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“…This is due to resistance enhanced by detoxifying enzymes such as mixed-function oxidases, glutathione-S-transferases, and esterases (Reyes et al, 2012). Those enzymes are found in several insects and make them resistant to different insecticides (Rodriguez et al, 2010;El-Latif et al, 2010;Wu et al, 2011). Erdogan and Babaroglu (2014) Esterases is non-specific enzyme implicated as an insecticide resistance mechanism in several insect pests due to their ability to hydrolyze insecticidal esters as organophosphates and pyrethroids (Soleno et al, 2008;Rodriguez et al, 2010;El-Latif and Subrahmanyam, 2010;Wu et al, 2011).…”

Section: Insecticide Resistance Mechanisms Of T Absolutamentioning

confidence: 99%

“…Those enzymes are found in several insects and make them resistant to different insecticides (Rodriguez et al, 2010;El-Latif et al, 2010;Wu et al, 2011). Erdogan and Babaroglu (2014) Esterases is non-specific enzyme implicated as an insecticide resistance mechanism in several insect pests due to their ability to hydrolyze insecticidal esters as organophosphates and pyrethroids (Soleno et al, 2008;Rodriguez et al, 2010;El-Latif and Subrahmanyam, 2010;Wu et al, 2011). The glutathione-S-transferase (GST) enzyme activity was determined to facilitate 1.5-fold higher resistance of T. absoluta against four insecticides, indoxacarb, metaflumizone, spinosad, and chlorantraniliprole.…”

Section: Insecticide Resistance Mechanisms Of T Absolutamentioning

confidence: 99%

Tomato leafminer [(Tuta absoluta Meyrick) (Lepidoptera: Gelechiidae)] and its current ecofriendly management strategies: A review

Aynalem¹

2018

J. Agric. Biotech. Sustain. Dev.

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Continue climatic changes in the world enhance the emergence of invasive agricultural insect pests.Tuta absoluta is one of the most devastating tomato leafminer and it spreads extensively in almost all parts of the world. It is a serious threat for tomato production and it results in highest damage. T. absoluta has fast growth rate with developmental stages of egg, larvae, pupa, and adult and it adapts to different environmental conditions. A larva is the most destructive one that consumes preferably leaves, stems and fruits of the tomato plant by hiding within mesophyll of the plant tissues. Although different environmentally hazardous pesticides are used against T. absoluta, none of the insecticides give full control due to the resistance development. This problem calls an urgent search for eco-friendly alternatives to control this invasive insect. Recent investigations are focused on different environmental friendly approaches that inclusively termed as IPM strategies. These are cultural practices, biopesticides, and biological using parasitoids, predators, microbial cells, microbial products, RNAi, inheritance sterility development in insect and pest resistant plant cultivar production are considered as promising alternatives to control T. absoluta. Therefore, this review briefly describes the current status of these methods used to design suitable and sustainable management strategies against T. absoluta.

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Overexpressed esterases in a fenvalerate resistant strain of the cotton bollworm, Helicoverpa armigera

Cited by 92 publications

References 46 publications

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Venom allergen 5is Associated With Deltamethrin Resistance inCulex pipiens pallens(Diptera: Culicidae)

Seasonal incidence and enzyme-based susceptibility to synthetic insecticides in two upcoming sucking insect pests of tea

Tomato leafminer [(Tuta absoluta Meyrick) (Lepidoptera: Gelechiidae)] and its current ecofriendly management strategies: A review

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