Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis

Li, Zhen; Han, Xiaojiao; Song, Xixi; Zhang, Yunxing; Jiang, Jing; Han, Qiang; Liu, Mingying; Qiao, Guirong; Zhuo, Renying

doi:10.3389/fpls.2017.01010

Cited by 82 publications

(43 citation statements)

References 48 publications

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“…Additionally, some studies have been carried out on the molecular mechanism underlying the hyper-accumulation and hyper-tolerance to heavy metals in S. alfredii . Overexpression of the genes SaMT2 , SaCu/Zn SOD , and SaREF , isolated from S. alfredii , conferred a higher tolerance to Cd stress in transgenic tobacco or A. thaliana [ 31 , 32 , 33 ]. Nevertheless, the Hsf family in S. alfredii has not yet been systematically investigated through phylogenetic, gene structure, conserved motif, and expression profiling analyses.…”

Section: Introductionmentioning

confidence: 99%

Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Chen

Jiang

Han

et al. 2018

IJMS

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Sedum alfredii Hance, a cadmium (Cd)/zinc (Zn)/lead (Pb) co-hyperaccumulating species, is a promising phytoremediation candidate because it accumulates substantial amounts of heavy metal ions without showing any obvious signs of poisoning. The heat shock transcription factor (Hsf) family plays crucial roles in plant growth, development, and stress responses. Although the roles of some Hsfs in abiotic stress have been well studied in model plants, the Hsf family has not been systematically investigated in heavy metal hyperaccumulators. Here, we comprehensively analyzed the Hsf gene family in S. alfredii based on a transcriptome under Cd stress. There were 22 Hsf members that were identified and phylogenetically clustered into three classes, namely, SaHsfA, SaHsfB, and SaHsfC. All of the three classes shared similar motifs. The expression profiles of the 22 Hsf members showed significant differences: 18 SaHsfs were responsive to Cd stress, as were multiple SaHsp genes, including SaHsp18.1, SaHsp22, SaHsp26.5, SaHsp70, SaHsp90, and SaHsp101. Two class A4 members, SaHsfA4a and SaHsfA4c, exhibited transcriptional activation activities. Overexpression of SaHsfA4a and SaHsfA4c in transgenic yeast indicated an improved tolerance to Cd stress and Cd accumulation. Our results suggest SaHsfs play important regulatory roles in heavy metal stress responses, and provide a reference for further studies on the mechanism of heavy metal stress regulation by SaHsfs.

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Section: Introductionmentioning

confidence: 99%

Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Chen

Jiang

Han

et al. 2018

IJMS

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“…Most notably, copper/zinc superoxide dismutase 1 (SOD1), CBL-interacting protein kinase 1(CIPK1) and dehydroascorbate reductase 2 (DHAR2) were overexpressed in SC56 under both conditions and have been found to play a role in stress tolerance. As mentioned, SOD1 is a crucial ROS scavenging enzyme, and was shown to enhance oxidative stress tolerance via transgenic overexpression in tobacco [16] and in Arabidopsis [49]. Similarly, as discussed, CIPK1 is a regulatory protein that is a convergent point in ABA-dependent and ABA independent stress tolerance [27].…”

Section: Sc56 Overexpresses Drought Tolerance Genes Under Wet Conditionsmentioning

confidence: 83%

Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy

2020

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Drought tolerance is a crucial trait for crops to curtail the yield loss inflicted by water stress, yet genetic improvement efforts are challenged by the complexity of this character. The adaptation of sorghum to abiotic stress, its genotypic variability, and relatively small genome make this species well-suited to dissect the molecular basis of drought tolerance. The use of differential transcriptome analysis provides a snapshot of the bioprocesses underlying drought response as well as genes that might be determinants of the drought tolerance trait. RNA sequencing data were analyzed via gene ontology enrichment to compare the transcriptome profiles of two sorghum lines, the drought-tolerant SC56 and the drought-sensitive Tx7000. SC56 outperformed Tx7000 in wet conditions by upregulating processes driving growth and guaranteeing homeostasis. The drought tolerance of SC56 seems to be an intrinsic trait occurring through overexpressing stress tolerance genes in wet conditions, notably genes acting in defense against oxidative stress (SOD1, SOD2, VTC1, MDAR1, MSRB2, and ABC1K1). Similarly to wet conditions, under drought, SC56 enhanced its transmembrane transport and maintained growth-promoting mechanisms. Under drought, SC56 also upregulated stress tolerance genes that heighten the antioxidant capacity (SOD1, RCI3, VTE1, UCP1, FD1, and FD2), regulatory factors (CIPK1 and CRK7), and repressors of premature senescence (SAUL1). The differential expression analysis uncovered biological processes which upregulation enables SC56 to be a better accumulator of biomass and connects the drought tolerance trait to key stress tolerance genes, making this genotype a judicious choice for isolation of tolerance genes.

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“…Most notably, copper/zinc superoxide dismutase 1 (SOD1), CBL-interacting protein kinase 1(CIPK1) and dehydroascorbate reductase 2 (DHAR2) were overexpressed in SC56 under both conditions and have been found to play a role in stress tolerance. As mentioned, SOD1 is a crucial ROS scavenging enzyme, and was shown to enhance oxidative stress tolerance via transgenic overexpression in tobacco [43] and in Arabidopsis [84]. Similarly, as discussed, CIPK1 is a regulatory protein that is a convergent point in ABAdependent and ABA independent stress tolerance [55].…”

Section: Sc56 Predisposed To Drought Tolerance Under Wet Conditionsmentioning

confidence: 84%

Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy

Olden¹,

Hunt²,

Dinkins

2019

Preprint

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Background Drought tolerance is a crucial trait for crops to curtail the yield loss inflicted by water stress to crops, yet genetic improvement efforts are challenged by the complexity of this character. The adaptation of sorghum to abiotic stress, its genotypic variability, and relatively small genome make this species well-suited to dissect the molecular basis of drought tolerance. One efficient approach to this question is the use of differential transcriptome analysis, which provides a snapshot of the processes underlying drought response as well as genes that might be determinants of the drought tolerance trait. Results RNA sequencing was used to compare the transcriptome profiles of two sorghum lines, the drought-tolerant SC56 and the drought-sensitive Tx7000. The differential expression analysis revealed unambiguous genotypic disparities, including a massive increase of upregulated transcripts in SC56. Concomitantly, gene ontology enrichment showed that SC56 biologically outperformed Tx7000 in wet conditions, since it upregulated processes driving growth and guaranteeing homeostasis. The drought tolerance of SC56 seems to be an intrinsic trait that occurs through the overexpression of stress tolerance genes in wet conditions, notably those acting in defense against oxidative stress (SOD1, SOD2, VTC1, MDAR1, MSRB2, and ABC1K1). Under drought conditions, SC56 enhanced its transmembrane transport and maintained growth-promoting mechanisms similar to those implemented under wet conditions. SC56 also appears to preserve its biological function, in a limiting environment, by relying on reported validated stress tolerance genes that heighten the antioxidant capacity (SOD1, RCI3, VTE1, UCP1, FD1, and FD2), regulatory factors (CIPK1 and CRK7), and repressors of premature senescence (SAUL1). Of the stress tolerance genes overexpressed under both wet and drought conditions, DHAR2 might be a key determinant of drought tolerance since its role in recycling ascorbic acid was described to be directly linked to protection against reactive oxygen species-mediated damage, positive effects on photosynthetic activity, higher rate of plant growth, and delayed leaf aging. Conclusion The differential expression analysis uncovered biological processes which upregulation enables SC56 to be a better accumulator of biomass and connects the drought tolerance trait to key stress tolerance genes, making this genotype a judicious choice for isolation of tolerance genes.

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Overexpressing the Sedum alfredii Cu/Zn Superoxide Dismutase Increased Resistance to Oxidative Stress in Transgenic Arabidopsis

Cited by 82 publications

References 48 publications

Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Identification, Expression Analysis of the Hsf Family, and Characterization of Class A4 in Sedum Alfredii Hance under Cadmium Stress

Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy

Transcriptome analysis of drought-tolerant sorghum genotype SC56 in response to water stress reveals an oxidative stress defense strategy

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