Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

Cheng, Hongsheng; Chen, Xiang; Zhu, Jianshun; Hong, Huang

doi:10.3389/fpls.2016.01703

Cited by 21 publications

(26 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The organ size of plants is related to cellular processes, such as cell proliferation and expansion (Horváth et al, 2006;Du et al, 2014;Cheng et al, 2016). The expression of MSI4 was up-regulated in TsNAC1 OX lines, and Arabidopsis MSI4 mutants showed a severalfold increase in vegetative biomass and a 1.3-fold expansion in cell size (Morel et al, 2009).…”

Section: Tsnac1 Retards Growth By Restricting Cell Expansion and Orgamentioning

confidence: 99%

TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth

Liu

Wang

et al. 2017

Plant Physiol.

View full text Add to dashboard Cite

NAC proteins constitute one of the largest families of plant-specific transcription factors, and a number of these proteins participate in the regulation of plant development and responses to abiotic stress. (), cloned from the halophyte , is a transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both and Arabidopsis () and retard the growth of these plants. In this study, the transcriptional activation activity of TsNAC1 and RD26 from Arabidopsis was compared with the target genes' promoter regions of TsNAC1 from , and the results showed that the transcriptional activation activity of TsNAC1 was higher in tobacco () and yeast. The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as , and the transcription factors and In addition, TsNAC1 negatively regulates the expansion of cells, inhibits and , and directly controls the expression of Based on these results, we propose that TsNAC1 functions as an important upstream regulator of plant abiotic stress responses and vegetative growth.

show abstract

Section: Tsnac1 Retards Growth By Restricting Cell Expansion and Orgamentioning

confidence: 99%

TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth

Liu

Wang

et al. 2017

Plant Physiol.

View full text Add to dashboard Cite

show abstract

“…This antagonism suggested that EBP1 provides an important link between a growth driver and cell cycle regulation (Horváth et al, 2006). The initial finding that EBP1 enhances growth was verified for a number of EBP1 orthologs identified in different species (Cao et al, 2009;Cheng et al, 2016;Wang et al, 2016); however, another report described an opposite effect (Li et al, 2016). Additionally, EBP1 expression is induced upon abiotic stress, and when EPB1 levels are elevated, it can confer stress tolerance (Cao et al, 2009;Cheng et al, 2016).…”

mentioning

confidence: 97%

ErbB-3 BINDING PROTEIN 1 Regulates Translation and Counteracts RETINOBLASTOMA RELATED to Maintain the Root Meristem

et al. 2019

View full text Add to dashboard Cite

The ErbB-3 BINDING PROTEIN 1 (EBP1) drives growth, but the mechanism of how it acts in plants is little understood. Here, we show that EBP1 expression and protein abundance in Arabidopsis (Arabidopsis thaliana) are predominantly confined to meristematic cells and are induced by sucrose and partially dependent on TARGET OF RAPAMYCIN (TOR) kinase activity. Consistent with being downstream of TOR, silencing of EBP1 restrains, while overexpression promotes, root growth, mostly under sucrose-limiting conditions. Inducible overexpression of RETINOBLASTOMA RELATED (RBR), a sugar-dependent transcriptional repressor of cell proliferation, depletes meristematic activity and causes precocious differentiation, which is attenuated by EBP1. To understand the molecular mechanism, we searched for EBP1-and RBR-interacting proteins by affinity purification and mass spectrometry. In line with the double-stranded RNA-binding activity of EBP1 in human (Homo sapiens) cells, the overwhelming majority of EBP1 interactors are part of ribonucleoprotein complexes regulating many aspects of protein synthesis, including ribosome biogenesis and mRNA translation. We confirmed that EBP1 associates with ribosomes and that EBP1 silencing hinders ribosomal RNA processing. We revealed that RBR also interacts with a set of EBP1-associated nucleolar proteins as well as factors that function in protein translation. This suggests EBP1 and RBR act antagonistically on common processes that determine the capacity for translation to tune meristematic activity in relation to available resources.

show abstract

“…To construct the RNA-Seq cDNA library, total RNA was extracted from 2-week-old dark-grown seedlings as previously described (Cheng, Chen, Zhu, & Huang, 2016). Six Illumina cDNA libraries were prepared using the Illumina TruSeq RNA sample preparation kit following the manufacturer's instructions.…”

Section: Rna-seq and Bioinformatic Analysismentioning

confidence: 99%

Hydrogen peroxide facilitates Arabidopsis seedling establishment by interacting with light signalling pathway in the dark

Cheng

Chen

et al. 2019

Plant Cell & Environment

Self Cite

View full text Add to dashboard Cite

Light is essential for the plant establishment. Arabidopsis seedlings germinated in the dark cannot grow leaf and only have closed cotyledons. However, exogenous application of H2O2 can induce leaves (establishment) in the dark. Comparative transcriptomic analysis revealed that light‐responsive genes were activated by H2O2 treatment. These genes are functionally correlated with photosynthesis, photorespiration, and components of photosystem, such as antenna proteins and light‐harvesting chlorophyll proteins. We further found that application of H2O2 facilitates cell cycle by accelerating G2–M checkpoint transition in shoot apical meristem. Phytochrome‐mediated light signalling pathway was also involved in the H2O2‐facilitated establishment process. The constitutive photomorphogenesis 1 and phytochrome interacting factor 3 proteins were shown to be down‐regulated by H2O2 treatment and accordingly removed their inhibitory effects on photomorphogenesis in the dark. The crosstalk between oxidation and light signal pathways explains the mechanism that H2O2 regulates plant dark establishment. The endogenous photorespiratory H2O2 production was mimicked by overexpression of glycolate oxidase genes and supplement of substrate glycolate. As expected, seedling establishment was also induced by the endogenously produced H2O2 under dark condition. These findings also suggest that photorespiratory H2O2 production is at least partially involved in postgermination establishment.

show abstract

Overexpression of a Hevea brasiliensis ErbB-3 Binding protein 1 Gene Increases Drought Tolerance and Organ Size in Arabidopsis

Cited by 21 publications

References 49 publications

TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth

TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth

ErbB-3 BINDING PROTEIN 1 Regulates Translation and Counteracts RETINOBLASTOMA RELATED to Maintain the Root Meristem

Hydrogen peroxide facilitates Arabidopsis seedling establishment by interacting with light signalling pathway in the dark

Contact Info

Product

Resources

About