TsNAC1 Is a Key Transcription Factor in Abiotic Stress Resistance and Growth

Abstract

NAC proteins constitute one of the largest families of plant-specific transcription factors, and a number of these proteins participate in the regulation of plant development and responses to abiotic stress. (), cloned from the halophyte , is a transcription factor gene, and its overexpression can improve abiotic stress resistance, especially in salt stress tolerance, in both and Arabidopsis () and retard the growth of these plants. In this study, the transcriptional activation activity of TsNAC1 and RD26 from Arabidopsis was compared with the target genes' promoter regions of TsNAC1 from , and the results showed that the transcriptional activation activity of TsNAC1 was higher in tobacco () and yeast. The target sequence of the promoter from the target genes also was identified, and TsNAC1 was shown to target the positive regulators of ion transportation, such as , and the transcription factors and In addition, TsNAC1 negatively regulates the expansion of cells, inhibits and , and directly controls the expression of Based on these results, we propose that TsNAC1 functions as an important upstream regulator of plant abiotic stress responses and vegetative growth.

TsHD1 and TsNAC1 cooperatively play roles in plant growth and abiotic stress resistance of Thellungiella halophile

“…Previous studies have demonstrated that the LSH1 and TXT2 expression were repressed in the TsNAC1 overexpression line (Liu et al ., ). In this study, clustering analysis of overlapped genes in the H, N and HN lines revealed that the differential expression levels of LSH1 , TXT2 , PME6 and PME35 correlated with the degree of inhibition of plant growth (Figure a).…”

“…TsNAC1 was reported to recognize CA(T/A)G, which repressed the expression of LIGHT-DEPENDENT SHORT HYPOCOTYLS 1 (LSH1) and UDP-XYLOSYLTRANSFERASE 2 (TXT2) (Liu et al, 2018). ZFHD1 specifically bound to T(T/ A)AATT in Arabidopsis .…”

“…Growth of the clones on the SD/ÀHis/ÀLeu/ÀTrp/ÀAde medium with different concentrations of aureobasidin A (AbA) also indicated that the heterodimers showed the highest stability ( Figure 3a). Previous studies have identified that TsNAC1 could activate TsVP1 expression by recognizing the only CATG (À549 bp) site in the À669 bp to À523 bp region of the TsVP1 promoter (Sun et al, 2010;Liu et al, 2018), and with no T(T/A)AATT site in this region. When the binding sites (orange for CATG, blue for TAAATT) in this region were mutated, four segments with mutated sequences (a, CATGGATAAGCATG; b, CATGGATAAG-TAAATT; c, TAAATTGATAAGTAAATT; d, GATAAG) were produced and connected to the pLacZi as reporters (Figure 3b).…”

“…Halophyte Thellungiella halophila and its transgenic homozygous lines with overexpressed TsHD1, overexpressed TsNAC1, overexpressed TsNAC1 and TsHD1, respectively, were used. The transgenic lines were produced as detailed in Liu et al (2018).…”

May the Fittest Protein Evolve: Favoring the Plant‐Specific Origin and Expansion of NAC Transcription Factors

“…Full length Full length protein localizes to both nucleus and plasma membrane; truncated N-terminal form devoid of TM motif is localized to the nucleus - [159] 70 TsNAC1 (Thellungiella halophila) Full length and C-terminal -Role in growth and abiotic stress resistance [163] 71 VND1-5/VASCULAR RELATED NAC-DOMAIN (Arabidopsis thaliana)…”