TsHD1 and TsNAC1 cooperatively play roles in plant growth and abiotic stress resistance of <i>Thellungiella halophile</i>

Liu, Can; Ma, Haizhen; Zhou, Jie; Li, Zhaoxia; Peng, Zhenghua; Guo, Fei; Zhang, Juren

doi:10.1111/tpj.14310

Cited by 17 publications

(13 citation statements)

References 54 publications

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“…The NAC recognition sequence CGT(G/A) and core DNA‐binding sequence CACG have been identified in the promoters of NAC‐targeted genes (Li et al ., 2019; Liu et al ., 2019; Xiang et al ., 2021). The putative ZmNAC49 binding elements were searched in the ZmSODs promoters and screened using luciferase assays.…”

Section: Resultsmentioning

confidence: 99%

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

et al. 2021

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Mitogen-activated protein kinase (MPK) is a critical regulator of the antioxidant defence system in response to various stimuli. However, how MPK directly and exactly regulates antioxidant enzyme activities is still unclear. Here, we demonstrated that a NAC transcription factor ZmNAC49 mediated the regulation of antioxidant enzyme activities by ZmMPK5.ZmNAC49 expression is induced by oxidative stress. ZmNAC49 enhances oxidative stress tolerance in maize, and it also reduces superoxide anion generation and increases superoxide dismutase (SOD) activity. A detailed study showed that ZmMPK5 directly interacts with and phosphorylates ZmNAC49 in vitro and in vivo. ZmMPK5 directly phosphorylates Thr-26 in NAC subdomain A of ZmNAC49. Mutation at Thr-26 of ZmNAC49 does not affect the interaction with ZmMPK5 and its subcellular localisation.Further analysis found that ZmNAC49 activates the ZmSOD3 expression by directly binding to its promoter. ZmMPK5-mediated ZmNAC49 phosphorylation improves its ability to bind to the ZmSOD3 promoter. Thr-26 of ZmNAC49 is essential for its transcriptional activity. In addition, ZmSOD3 enhances oxidative stress tolerance in maize.Our results show that phosphorylation of Thr-26 in ZmNAC49 by ZmMPK5 increased its DNA-binding activity to the ZmSOD3 promoter, enhanced SOD activity and thereby improved oxidative stress tolerance in maize.

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Section: Resultsmentioning

confidence: 99%

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

et al. 2021

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“…Moreover, the dimerization between the subdomain of NAC and other TFs plays an important role in the heat stress response. For example, TsHD1 cloned from the halophyte Thellungiella halophila, is a homeodomain (HD) TF gene that can improve heat tolerance by forming heterodimers through the interaction between the TsHD1 zinc finger domain and the A subdomain of TsNAC1 (Liu et al, 2019). Heat shock TFs (HSFs) and heat shock proteins (HSPs), which act as molecular chaperones for HSFs, are key TFs that respond to heat stress, NACs are likely to function as upstream regulators of HSFs (Figure 3a).…”

Section: Low-/high-temperature Stressmentioning

confidence: 99%

NACs, generalist in plant life

Han,

Zhao,

Sun

et al. 2023

Plant Biotechnology Journal

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SummaryPlant‐specific NAC proteins constitute a major transcription factor family that is well‐known for its roles in plant growth, development, and responses to abiotic and biotic stresses. In recent years, there has been significant progress in understanding the functions of NAC proteins. NAC proteins have a highly conserved DNA‐binding domain; however, their functions are diverse. Previous understanding of the structure of NAC transcription factors can be used as the basis for their functional diversity. NAC transcription factors consist of a target‐binding domain at the N‐terminus and a highly versatile C‐terminal domain that interacts with other proteins. A growing body of research on NAC transcription factors helps us comprehend the intricate signalling network and transcriptional reprogramming facilitated by NAC‐mediated complexes. However, most studies of NAC proteins have been limited to a single function. Here, we discuss the upstream regulators, regulatory components and targets of NAC in the context of their prospective roles in plant improvement strategies via biotechnology intervention, highlighting the importance of the NAC transcription factor family in plants and the need for further research.

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“…NAC TFs play important regulatory roles in various aspects of plant growth, development, and adaptation to the environment, including in shoot apical meristem formation [5], cell division and expansion [6,7] nutrient remobilization [8], flower formation [9], lateral root development [10,11], leaf senescence [12][13][14][15][16], secondary cell wall biosynthesis [3,[17][18][19][20], fiber development [19,21,22], fruit ripening [23,24], seed development [25], response to pathogen infection [26][27][28][29][30], and abiotic stress tolerance [31][32][33]. Many studies have confirmed that many NAC genes play crucial roles in the regulation of plant tolerance to drought.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the NAC transcription factor family in broomcorn millet (Panicum miliaceum L.) and expression analysis under drought stress

Shan

Jiang

et al. 2020

BMC Genomics

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Background: Broomcorn millet is a drought-tolerant cereal that is widely cultivated in the semiarid regions of Asia, Europe, and other continents; however, the mechanisms underlying its drought-tolerance are poorly understood. The NAM, ATAF1/2, and CUC2 (NAC) transcription factors form a large plant-specific gene family that is involved in the regulation of tissue development and abiotic stress. To date, NAC transcription factors have not been systematically researched in broomcorn millet.Results: In the present study, a total of 180 NAC (PmNAC) genes were identified from the broomcorn millet genome and named uniformly according to their chromosomal distribution. Phylogenetic analysis demonstrated that the PmNACs clustered into 12 subgroups, including the broomcorn millet-specific subgroup Pm_NAC. Gene structure and protein motif analyses indicated that closely clustered PmNAC genes were relatively conserved within each subgroup, while genome mapping analysis revealed that the PmNAC genes were unevenly distributed on broomcorn millet chromosomes. Transcriptome analysis revealed that the PmNAC genes differed greatly in expression in various tissues and under different drought stress durations. The expression of 10 selected genes under drought stress was analyzed using quantitative real-time PCR. Conclusion: In this study, 180 NAC genes were identified in broomcorn millet, and their phylogenetic relationships, gene structures, protein motifs, chromosomal distribution, duplication, expression patterns in different tissues, and responses to drought stress were studied. These results will be useful for the further study of the functional characteristics of PmNAC genes, particularly with regards to drought resistance.

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TsHD1 and TsNAC1 cooperatively play roles in plant growth and abiotic stress resistance of Thellungiella halophile

Cited by 17 publications

References 54 publications

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

ZmMPK5 phosphorylates ZmNAC49 to enhance oxidative stress tolerance in maize

NACs, generalist in plant life

Genome-wide analysis of the NAC transcription factor family in broomcorn millet (Panicum miliaceum L.) and expression analysis under drought stress

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