Overexpression of a Laccase with Dye Decolorization Activity from &lt;b&gt;&lt;i&gt;Bacillus&lt;/i&gt;&lt;/b&gt; sp. Induced in &lt;b&gt;&lt;i&gt;Escherichia coli&lt;/i&gt;&lt;/b&gt;

Guo, Haipeng; Zheng, Bingsong; Jiang, Dean; Qin, Wensheng

doi:10.1159/000478859

Cited by 9 publications

(3 citation statements)

References 55 publications

(69 reference statements)

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“…In addition, screening for variants of laccase genes in the genome of basidiomycetes, bacteria, and plants, combined with the use of rational directed evolution approaches, has proven to be efficient for enhanced laccase production and activity. [95][96][97][98][99] Native laccases have been isolated from various domains of life, but the culture media used to isolate and routinely maintain cells for laccase screening selects only "culturable" organisms that represent a small fraction of the wealth of recognized organisms with putative laccase sequences as predicted from metagenomic studies. On the other hand, the development of novel media formulations is expensive and time-consuming, and high proportions of organisms from different taxa remain uncultured.…”

Section: Recombinant Dna Approaches To Combat Toxic Lignin Derivativesmentioning

confidence: 99%

“…In general, heterologous expression approaches of laccase genes include plasmid‐borne or chromosomal integration of these genes under the control of native, surrogate, or synthetic promoters. In addition, screening for variants of laccase genes in the genome of basidiomycetes, bacteria, and plants, combined with the use of rational directed evolution approaches, has proven to be efficient for enhanced laccase production and activity 95–99 …”

Section: Recombinant Dna Approaches To Combat Toxic Lignin Derivativesmentioning

confidence: 99%

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Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

Fernández‐Sandoval,

García,

Teymennet‐Ramírez

et al. 2023

Biotechnology Progress

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Lignocellulose is the most abundant biopolymer in the biosphere. It is inexpensive and therefore considered an attractive feedstock to produce biofuels and other biochemicals. Thermochemical and/or enzymatic pretreatment is used to release fermentable monomeric sugars. However, a variety of inhibitory by‐products such as weak acids, furans, and phenolics that inhibit cell growth and fermentation are also released. Phenolic compounds are among the most toxic components in lignocellulosic hydrolysates and slurries derived from lignin decomposition, affecting overall fermentation processes and production yields and productivity. Ligninolytic enzymes have been shown to lower inhibitor concentrations in these hydrolysates, thereby enhancing their fermentability into valuable products. Among them, laccases, which are capable of oxidizing lignin and a variety of phenolic compounds in an environmentally benign manner, have been used for biomass delignification and detoxification of lignocellulose hydrolysates with promising results. This review discusses the state of the art of different enzymatic approaches to hydrolysate detoxification. In particular, laccases are used in separate or in situ detoxification steps, namely in free enzyme processes or immobilized by cell surface display technology to improve the efficiency of the fermentative process and consequently the production of second‐generation biofuels and bio‐based chemicals.

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Section: Recombinant Dna Approaches To Combat Toxic Lignin Derivativesmentioning

confidence: 99%

Section: Recombinant Dna Approaches To Combat Toxic Lignin Derivativesmentioning

confidence: 99%

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

Fernández‐Sandoval,

García,

Teymennet‐Ramírez

et al. 2023

Biotechnology Progress

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show abstract

“…4) Several dyes including indigo carmine and orange G were decomposable with extracellularly expressed cotA laccase [52]. Congo red, Coomassie Brilliant Blue R, and crystal violet were decolorized by intracellularly expressed cotA laccase [53]. Malachite Green, Acid Blue 62, and methyl orange were also decolorized by surface-displayed cotA laccase using E. coli.…”

Section: Laccase Activitymentioning

confidence: 99%

Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores

Park¹,

Kim²,

Lee³

et al. 2019

Journal of Microbiology and Biotechnology

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Introduction Approximately 10,000 different dyes and pigments are used industrially, and over 7 × 10 5 tons of synthetic dyes are produced annually worldwide [1, 2]. Synthetic dyes are extensively used in textile dyeing, paper printing, color photography, pharmaceutical, food, cosmetics, and other industries [3]. Approximately 128 tons of dye wastewater is discharged daily and high cost is incurred for its removal. Wastewater remediation is challenging because it contains a variety of pollutants and it is difficult to change the composition of the contaminants. Physical, chemical, and biological methods have been widely used to decolorize dye wastewater [4]. The physical and chemical treatment methods are preferred over other methods; however, these methods require high operating cost and other accessory equipment. It would be desirable to develop a new decolorization method that is efficient and cost effective to reduce the overall cost of dye wastewater treatment. Recently, many studies have been published on the decolorization of synthetic dyes by laccase [5, 6]. Laccase was first discovered in 1883 from Rhus vernicifera, the Japanese lacquer tree [7]. Laccases (benzenediol: oxygen oxidoreductase; E.C. 1.10.3.2) are a family of multi-copper oxidases (MCOs) that require oxygen to oxidize organic compounds-particularly phenols and non-phenolic aromatic substrates-by one-electron transfer to form reactive radicals,

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Multiple Applications of Enzymes Induced by Algal Biomasses from a New Bacillus Isolate to Saccharify Algae and Degrade Chemical Dyes

Guo

Zhang

et al. 2018

Waste Biomass Valor

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Overexpression of a Laccase with Dye Decolorization Activity from <b><i>Bacillus</i></b> sp. Induced in <b><i>Escherichia coli</i></b>

Cited by 9 publications

References 55 publications

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

Removal of phenolic inhibitors from lignocellulose hydrolysates using laccases for the production of fuels and chemicals

Decolorization of Acid Green 25 by Surface Display of CotA laccase on Bacillus subtilis spores

Multiple Applications of Enzymes Induced by Algal Biomasses from a New Bacillus Isolate to Saccharify Algae and Degrade Chemical Dyes

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