Overexpression of a maize dehydrin gene, ZmDHN2b, in tobacco enhances tolerance to low temperature

Xing, Xin‐Hui; Liu, Yukun; Kong, Xiangpei; Yang, Lin-Cheng; Li, Dequan

doi:10.1007/s10725-011-9580-3

Cited by 55 publications

(36 citation statements)

References 48 publications

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“…SK n and K n -type DHNs are closely related to cold acclimation (Rorat 2006). Therefore, considering the association of SK 3 -type DHN genes to cold tolerance (Danyluk et al 1994;Houde et al 2004;Rorat 2006;Yin et al 2006;Xing et al 2011), Cucurbitaceae SK 3 -type DHNs in Group I might be important for cold tolerance (Fig. 1).…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Lee

Ali

et al. 2017

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT Dehydrins (DHNs) are hydrophilic proteins with conserved lysine-rich K-segment, which belong to Group II of the late embryogenesis abundant (LEA) protein family. DHNs are considered as molecular chaperons playing important roles in abiotic stress tolerance. In this study, DHN genes were identified through genome-wide searches in five Cucurbitaceae species, including cucumber, wild cucumber, melon, watermelon, and bitter gourd. Three to five DHN genes were found in each of the five species, which were further divided into several protein architecture types based on the presence and order of the major conserved motifs such as K-, Y-, and S-segments. In silico expression profiling using RNA-Seq data revealed high expression of SK3-type DHN gene and low expression of other type DHN genes in cucumber and melon. In silico promoter analysis identified a number of cis-acting element-like sequences related to abiotic stress-response such as DRE and ABRE in 2-kb putative promoter sequences. DHN genes identified in this study will be valuable for understanding the stress response mechanism as well as assisting molecular breeding in Cucurbitaceae crops.

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Section: Discussionmentioning

confidence: 99%

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Lee

Ali

et al. 2017

Plant Breed. Biotech.

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“…MDA is the organic compound with the formula CH 2 (CHO) 2 and usually used as a marker for lipid peroxidation (Dong et al 2003). EL reflects membrane injury severity after abiotic stresses (Xing et al 2011). Under drought stress, the MbWRKY2-overexpression transgenic plants generated smaller amounts of MDA and H 2 O 2 , and had the lower EL levels than in WT (Figure 6).…”

Section: Discussionmentioning

confidence: 99%

“…Leaf relative water content (RWC) was estimated according to RWC (%) = (fresh weight -dry weight)/(turgid weight -dry weight)×100 (Wang et al 2009). Electrolyte leakage (EL) in tobacco leaves was determined following the protocols described by Xing et al (2011).…”

Section: Measurement the Levels Of Relative Water Content And Electromentioning

confidence: 99%

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Han

Zhang

Ding

et al. 2018

Journal of Plant Interactions

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Plant-specific WRKY transcription factors are widely involved in abiotic stress responses. In this study, a WRKY gene was isolated from Malus baccata (L.) Borkh and designated it as MbWRKY2. Subcellular localization showed that MbWRKY2 was localized in the nucleus. The expression levels of MbWRKY2 were up-regulated by abiotic stresses in M. baccata. When MbWRKY2 was introduced into tobacco, it improved drought stress tolerance in transgenic plants. The transgenic tobaccos had the higher contents of chlorophyl, proline, relative water content, AsA, and GSH, increased activities of CAT, APX, SOD, and POD, and decreased levels of MDA, H 2 O 2 , and electrolyte leakage than wild-type, especially when dealt with dehydration treatment. Moreover, the MbWRKY2-OE plants enhanced the expression of oxidative stress response and stress-related genes involved in osmotic adjustment and membrane protection. These results suggest that MbWRKY2 gene plays a positive regulatory role in drought stress response. ARTICLE HISTORY

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“…The expressed products were separated by electrophoresis in a 10% sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) [16].…”

Section: Prokaryotic Expression Analysismentioning

confidence: 99%

“…Thermal dissipation acts as a main way to alleviate photoinhibition [11,31]. NPQ is a light-inducible process that competes and diverts absorbed energy from photochemistry to balance light harvesting and photosynthetic performance [16,32]. NPQ has been shown to be quickly responsive to high light stress and functional in preventing the over-excitation of photochemical reactions via dissipating excessive excitation energy into heat [11,33].…”

Section: Effects Of Chilling-low Light Treatment On Npq and Fv/fm Of mentioning

confidence: 99%

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

Liu

Yao

et al. 2017

Biotechnology & Biotechnological Equipment

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In this study, a watermelon (Citrullus lanatus) zeaxanthin epoxidase gene, ClZE, was isolated by reverse transcription-polymerase chain reaction (PCR) together with RNA ligase-mediated rapid amplification of cDNA ends (RLM-RACE). The full cDNA sequence of ClZE is 2535 bp in length containing a 1998 bp open reading frame (ORF) that encodes 665 amino acids. ClZE was shown to share high homology with the putative ZE genes in other plant species. Prediction analysis revealed that ClZE bears two large conservative domains, including Pyr_redox and FHA (forkheadassociated domain). Phylogenetic analysis suggested that ClZE shares high similarity (94.8%) with CsZE from Cucumis sativus, but is far from the ZE genes of other species. Prokaryotic expression indicated that ClZE possessed the apparent molecular mass consistent with its calculated molecular mass of 73.1 KDa. Based on quantitative real-time PCR (qRT-PCR), ClZE was shown to be down-regulated under chilling-low irradiance stress in watermelon leaves. Transgenic Arabidopsis lines harbouring ClZE were generated to test the gene function in mediating plant response to chilling stress. The results indicated that the transgenic lines exhibited decreased nonphotochemical quenching (NPQ) and maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm), increased activities of peroxidase (POD), superoxide dismutase (SOD) and elevated content of malondialdehyde (MDA) relative to the wild type (WT) under chilling-low irradiance stress. In addition, overexpression of ClZE resulted in impaired xanthophyll cycle and aggravated PSII photoinhibition under chilling-low irradiance. All the results together suggested that ClZE plays major roles in regulating the photoinhibition behaviour.

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Overexpression of a maize dehydrin gene, ZmDHN2b, in tobacco enhances tolerance to low temperature

Cited by 55 publications

References 48 publications

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Genome-Wide Identification of the Dehydrin Genes in the Cucurbitaceae Species

Isolation and characterization of MbWRKY2 gene involved in enhanced drought tolerance in transgenic tobacco

Molecular cloning and characterization of ClZE, a zeaxanthin epoxidase gene in watermelon (Citrullus lanatus)

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