Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress

Sripinyowanich, Siriporn; Chamnanmanoontham, Nontalee; Udomchalothorn, Thanikarn; Maneeprasopsuk, Somporn; Santawee, Panudda; Buaboocha, Teerapong; Liu, Qu; Gu, Hongya; Chadchawan, Supachitra

doi:10.1016/j.plantsci.2013.08.013

Cited by 23 publications

(27 citation statements)

References 45 publications

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“…Nucleolin is found in a diverse array of organisms ranging from yeast to plants to mammals. It is involved in rRNA processing, ribosome assembly, transcriptional repression, and transport of ribosomes to the cytoplasm [49], and may promote plant growth [50]. Nucleolin and nucleophosmin do not share any structural homology although they have numerous functions in common.…”

Section: Resultsmentioning

confidence: 99%

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

Jiang

Zhang

Qin

et al. 2014

IJMS

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To study the toxic mechanisms of lead (Pb) in plants, the effects of Pb on the morphology and structure of the nucleolus in root tip meristematic cells of Allium cepa var. agrogarum L. were investigated. Fluorescence labeling, silver-stained indirect immunofluorescent microscopy and western blotting were used. Fluorescence labeling showed that Pb ions were localized in the meristematic cells and the uptake and accumulation of Pb increased with treatment time. At low concentrations of Pb (1–10 μM) there were persistent nucleoli in some cells during mitosis, and at high concentration (100 μM) many of the nucleolar organizing regions were localized on sticky chromosomes in metaphase and anaphase cells. Pb induced the release of particles containing argyrophilic proteins to be released from the nucleus into the cytoplasm. These proteins contained nucleophosmin and nucleolin. Pb also caused the extrusion of fibrillarin from the nucleus into the cytoplasm. Western blotting demonstrated the increased expression of these three major nucleolar proteins under Pb stress.

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Section: Resultsmentioning

confidence: 99%

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

Jiang

Zhang

Qin

et al. 2014

IJMS

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“…However, more studies are required in this direction. Nucleolin is found in yeasts, plants and mammals and involved in essential processes of ribosome biogenesis, and may interact directly to promote plant growth (Chathoth et al 2009, Sripinyowanich et al 2013. Fibrillarin is present both in animal and plant cells.…”

Section: Discussionmentioning

confidence: 99%

Effects of cadmium on nucleolus in root tips of Hordeum vulgare

et al. 2017

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Background: Hordeum vulgare is an economically important crop that is consumed in large quantities in the world. In a previous investigation, we found that cadmium (Cd) could induce some nucleolar materials containing the argyrophilic proteins scattered in the nuclei and located in the cytoplasm in the root tip cells of H. vulgare. However, what kinds of nucleolar proteins are affected has not been reported. In order to further confirm the cytological effects of Cd on nucleolus and nucleolar proteins, alterations in the cellular localization and expression of two major nucleolar proteins: fibrillarin and nucleolin in the root tips of H. vulgare were studied in the present investigation. Methods: Cytological effects of Cd on nucleolus were observed by silver-staining method and two nucleolarproteins: nucleolin and fibrillarin were observed and analyzed by means of indirect immunofluorescent microscopy and western blotting. Results:Results from the present investigation demonstrated that under Cd stress (cadmium chloride at 50 and 100 μM), Cd could disturb the nucleolar cycle with the extrusion of silver-stained materials containing argyrophilic proteins from the nucleolus into the cytoplasm. Nucleolar material containing nucleolin and fibrillarin and their movement into the cytoplasm following Cd stress was detected with indirect immunofluorescence techniques. Western blotting revealed higher expression of these two major nucleoproteins in Cd-treated roots, which was consistent with the results obtained by indirect immunofluorescence. Conclusion:The findings of the present study confirm that Cd can have toxic effects on nucleolus and affect the expression of nucleolar proteins, nucleolin and fibrillarin. The data obtained here can provide valuable information for monitoring and forecast early effects of exposure to Cd in polluted environments.

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“…C23 is an important multifunctional protein that has been implicated directly or indirectly in many metabolic processes and is one of the most abundant nonribosomal proteins of the nucleolus (Bugler et al, 1982). Sripinyowanich et al (2013) suggest that C23 and ribosomal proteins affect ribosome biogenesis independently or that C23 may interact directly to promote plant growth. It was proved that the retention of nucleolar proteins within the nucleolus was closely related to active rRNA synthesis (Martin et al, 2007;Chen and Jiang, 2004).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetical effects of aluminum on root meristem cells of Helianthus annuus L.

et al. 2015

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Different aluminum concentrations (5, 50, and 100 μM), as well as different exposure times (24, 48, and 72 h), were applied to investigate cytogenetical alterations in sunfl ower (Helianthus annuus L.) meristem cells. Nucleolin was also examined under aluminum stress using silver-staining method and indirect immunofl uorescent method. Results showed that the mitotic index decreased progressively when either aluminum concentration or exposure time increased. C-mitosis, anaphase bridges, and chromosome stickiness were observed in the aluminum treated root tip cells. Some particulates containing the argyrophilic NORassociated proteins were distributed in the nucleus of the root-tip cells, and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus into the cytoplasm. The result also indicated the nucleolar protein nucleolin was translocated from nucleolus to nucleoplasm and cytoplasm, that presence of 50 μM of aluminum for 48 h, suggesting that aluminum disturbed rRNA synthesis and induced the abnormal cellular location of nucleolar protein. The strongly toxic effect of aluminum on the nucleolus can inhibit or stop mitosis, and consequently inhibit root growth. Keywords: aluminum, cytogenetical alterations, mitosis, nucleolin, sunfl ower.Resumen: Diferentes concentraciones de aluminio (5, 50 y 100 μM) y diferentes tiempos de exposición (24, 48 y 72 h) se aplicaron a células meristemáticas de girasol (Helianthus annuus L.), para investigar las alteraciones citogenéticas, en particular, dilucidar sobre la nucleolina. La nucleolina fue estudiada bajo el estrés por aluminio, utilizando el método de tinción con plata y el método de inmunofl uorescencia indirecta. Los resultados mostraron que el índice mitótico disminuyó progresivamente a medida que aumentó la concentración de aluminio o el tiempo de exposición. Se observaron C-mitosis, puentes en la anafase y rigidez en los cromosomas en todas las células de la punta de la raíz tratadas con aluminio. Algunas partículas contienen argirófi las NOR asociadas a proteínas distribuidas en el núcleo de las células del ápice de la raíz. La cantidad de este material granulado aumentó progresivamente a medida que aumentó el tiempo de exposición. Finalmente, el material nucleolar se expulsó desde el núcleo hacia el citoplasma. El resultado también indica que, en presencia de 50 μM de aluminio por 48 h, la proteína nucleolina se transloca desde el nucleolo al nucleoplasma y citoplasma, lo que sugiere que el aluminio altera la síntesis de ARNr e induce la localización celular anormal de la proteína nucleolar. El fuerte efecto tóxico de aluminio en el nucléolo puede inhibir o detener la mitosis, y por consiguiente, inhibir el crecimiento de la raíz.

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Overexpression of a partial fragment of the salt-responsive gene OsNUC1 enhances salt adaptation in transgenic Arabidopsis thaliana and rice (Oryza sativa L.) during salt stress

Cited by 23 publications

References 45 publications

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

Effects of Lead on the Morphology and Structure of the Nucleolus in the Root Tip Meristematic Cells of Allium cepa L.

Effects of cadmium on nucleolus in root tips of Hordeum vulgare

Cytogenetical effects of aluminum on root meristem cells of Helianthus annuus L.

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