Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis

Kumari, P. Hima; Kumar, S. Anil; Pramod, S.; Ramesh, Katam; Suravajhala, Prashanth; Rao, K. S.; Varshney, Rajeev K.; Kishor, P. B. Kavi

doi:10.3389/fpls.2016.02027

Cited by 33 publications

(24 citation statements)

References 73 publications

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“…For Western blot, 20 μg tonoplast proteins were separated using 12 % (m/v) sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), and immunoblotted with antibody against polyclonal Na + /H + antiporter (AS09484, Agrisera, Vännäs, Sweden). Blots were performed according to methods of Xia et al (2002) and Kumari et al (2017).…”

Section: Isolation Of Tonoplast Vesicles Determination Of Vac U Olarmentioning

confidence: 99%

Overexpression of Iris lactea tonoplast Na+/H+ antiporter gene IlNHX confers improved salt tolerance in tobacco

Guo¹,

Tian²,

Mao³

et al. 2020

Biol. plant.

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Sodium cation compartmentalization into vacuoles is one of the effective strategies for adaptation of halophytes to saline environments. Tonoplast Na + /H + antiporter (NHX) is involved in Na + sequestration into vacuoles under salt stress. However, the function of NHX in halophyte Iris lactea is still unclear. In this study, a significant positive correlation was observed between Na + accumulations and IlNHX expression in tissues under 0-200 mM NaCl, indicating IlNHX might be responsible for Na + accumulation of I. lactea under salt stress. More important, IlNHX was specifically localized to the tonoplast. Transgenic tobacco expressing IlNHX grew better and showed higher tolerance to 200 mM NaCl than respective wild type (WT). Compared to WT, transgenic tobacco accumulated more Na + and K + and maintained higher K + /Na + ratios in tissues, accompanied by the reduction of chlorophyll loss and lipid peroxidation in the presence of NaCl. Moreover, transgenic tobacco exhibited markedly higher vacuolar H +-ATPase (V-ATPase) activity relative to WT when subjected to salt stress. The findings suggest that transgenic plants overexpressing IlNHX could compartmentalize more Na + into vacuoles in tobacco via enhanced V-ATPase activity, which further contributes to maintaining K + and Na + homeostasis, to improved photosynthesis, and to protection of cell membrane integrity under salt stress.

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Section: Isolation Of Tonoplast Vesicles Determination Of Vac U Olarmentioning

confidence: 99%

Overexpression of Iris lactea tonoplast Na+/H+ antiporter gene IlNHX confers improved salt tolerance in tobacco

Guo¹,

Tian²,

Mao³

et al. 2020

Biol. plant.

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“…Transcription factors such as NAC, bZIP, AP2/ERF, WRKY, MYB and bHLH regulated the expression of saltresponsive genes and ultimately determined the salt tolerance level of plants [25][26][27][28][29][30]69]. 12 TFs differentially expressed in both genotypes under NaCl treatment were indenti ed in the transcriptome analysis, including three MYBs (AUR62042086, AUR62034188 and AUR62030018), four NACs (AUR62000536, AUR62029344, AUR62015630 and AUR62006885), three WRKYs (AUR62030836, AUR62026996 and AUR62006427), and two ZFPs (AUR62042998 and AUR62009622).…”

Section: Transcription Factors Related To Salt Stress In Quinoamentioning

confidence: 99%

“…Plasma membrane bound SbNHXLP (a Na + /H + antiporter-like protein) from sorghum involves in Na + exclusion, maintains ion homeostasis and alleviates NaCl stress in transgenic tomato, and more notably, it increases the fruit yield of tomato [24]. Besides, various transcription factors (TFs) had been shown to participate in salt stress response, such as NAC [25], basic leucine zipper (bZIP) [26], APETALA2/ethylene response factor (AP2/ERF) [27], WRKY [28], basic helix-loop-helix (bHLH) [29] and MYB [30]. They regulate the expression levels of multiple downstream genes, which might ultimately affect salt tolerance of plants.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis and Differential Gene Expression Profiling of Two Contrasting Quinoa Genotypes in Response to Salt Stress

Shi¹,

Gu²

2020

Preprint

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Background: Soil salinity is one of the major abiotic stress factors that affect crop growth and yield, which seriously restricts the sustainable development of agriculture. Quinoa is considered as one of the most promising crops in the future for its high nutrition value and strong adaptability to extreme weather and soil conditions. However, the molecular mechanisms underlying the adaptive response to salinity stress of quinoa remain poorly understood. To identify candidate genes related to salt tolerance, we performed reference-guided assembly and compared the gene expression in roots treated with 300 mM NaCl for 0, 0.5, 2, and 24 h of two contrasting quinoa genotypes differing in salt tolerance.Results: The salt-tolerant (ST) genotype displayed higher seed germination rate and plant survival rate, and stronger seedling growth potential as well than the salt-sensitive (SS) genotype under salt stress. An average of 38,510,203 high-quality clean reads were generated. Significant Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were identified to deeper understand the differential response. Transcriptome analysis indicated that salt-responsive genes in quinoa were mainly related to biosynthesis of secondary metabolites, alpha-Linolenic acid metabolism, plant hormone signal transduction, and metabolic pathways. Moreover, several pathways were significantly enriched amongst the differentially expressed genes (DEGs) in ST genotypes, such as phenylpropanoid biosynthesis, plant-pathogen interaction, isoquinoline alkaloid biosynthesis, and tyrosine metabolism. 117 DEGs were common to various stages of both genotypes, identified as core salt-responsive genes, including some transcription factor members, like MYB, WRKY and NAC, and some plant hormone signal transduction related genes, like PYL, PP2C and TIFY10A, which play an important role in the adaptation to salt conditions of this species. The expression patterns of 21 DEGs were detected by quantitative real-time PCR (qRT-PCR) and confirmed the reliability of the RNA-Seq results. Conclusions: We identified candidate genes involved in salt tolerance in quinoa, as well as some DEGs exclusively expressed in ST genotype. The DEGs common to both genotypes under salt stress may be the key genes for quinoa to adapt to salinity environment. These candidate genes regulate salt tolerance primarily by participating in reactive oxygen species (ROS) scavenging system, protein kinases biosynthesis, plant hormone signal transduction and other important biological processes. These findings provide theoretical basis for further understanding the regulation mechanism underlying salt tolerance network of quinoa, as well establish foundation for improving its tolerance to salinity in future breeding programs.

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“…To exclude Na + or enable better K + retention in the cytosol, the plasma membrane H + -ATPase activity is rapidly upregulated upon salinity treatment in Atriplex lentiformis and Chenopodium quinoa (Bose et al, 2014 ). Many genes associated with the extrusion and/or intracellular compartmentalization of Na + , such as Arabidopsis thaliana Na + /H + antiporter 1 ( AtNHX1 ), Sorghum bicolor Na + / H + antiporter-like protein ( SbNHXLP ), A. thaliana salt overly sensitive 1 ( AtSOS1 ), A. thaliana salt overly sensitive 2 ( AtSOS2 ), Arabidopsis vacuolar pyrophosphatase 1 ( AVP1 ), and Osmotin like protein ( OLP ), have been identified (Guo et al, 2012 ; Kumar et al, 2015 , 2016 ; Peng et al, 2016 ; Kumari et al, 2017 ). SbNHXLP , a new member of the Na + /H + antiporter ( NHX ) gene family, is not only involved in excluding Na + from the cytoplasm but also in acquiring K + and maintaining a high K + /Na + ratio in transgenic tomato.…”

Section: Introductionmentioning

confidence: 99%

iTRAQ-Based Comparative Proteomic Analysis of Seedling Leaves of Two Upland Cotton Genotypes Differing in Salt Tolerance

Gong

Sun

et al. 2017

Front. Plant Sci.

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Cotton yields are greatly reduced under high salinity stress conditions, although cotton is considered a moderately salt-tolerant crop. Understanding at the molecular level how cotton responds to salt stress will help in developing salt tolerant varieties. Here, we combined physiological analysis with isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics of seedling leaves of 2 genotypes differing in salinity tolerance to 200 mM (18.3 dS/m) NaCl stress. Salt stress produced significant stress symptoms in the sensitive genotype Nan Dan Ba Di Da Hua (N), including lower relative water and chlorophyll contents and higher relative electrolyte leakage and Na+/K+ ratio in leaf samples, compared with those in the tolerant genotype Earlistaple 7 (Z). A total of 58 differentially abundant salt-responsive proteins were identified. Asp-Glu-Ala-Asp (DEAD)-box ATP-dependent RNA helicase 3 and protochlorophyllide reductase were markedly suppressed after salt treatment, whereas the phosphate-related differentially abundant proteins (DAPs) phosphoethanolamine N-methyltransferase 1 and 14-3-3-like protein E were induced, and all these proteins may play significant roles in salt stress. Twenty-nine salt-responsive proteins were also genotype specific, and 62.1 and 27.6% of these were related to chloroplast and defense responses, respectively. Based on the Arabidopsis thaliana protein interaction database, orthologs of 25 proteins showed interactions in Arabidopsis, and among these, a calmodulin protein was predicted to have 212 functional partners. In addition, the Golgi apparatus and calcium may be important for salt secretion in cotton. Through integrative proteome and transcriptome analysis, 16 DAPs were matched to differentially expressed genes and verified using qRT-PCR. On the basis of these findings, we proposed that some proteins related to chloroplast, ATP, ribosomal, and phosphate metabolism as well as to the Golgi apparatus and calcium may play key roles in the short-term salt stress response of cotton seedling leaves.

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Overexpression of a Plasma Membrane Bound Na+/H+ Antiporter-Like Protein (SbNHXLP) Confers Salt Tolerance and Improves Fruit Yield in Tomato by Maintaining Ion Homeostasis

Cited by 33 publications

References 73 publications

Overexpression of Iris lactea tonoplast Na<sup>+</sup>/H<sup>+</sup> antiporter gene IlNHX confers improved salt tolerance in tobacco

Overexpression of Iris lactea tonoplast Na<sup>+</sup>/H<sup>+</sup> antiporter gene IlNHX confers improved salt tolerance in tobacco

Transcriptome Analysis and Differential Gene Expression Profiling of Two Contrasting Quinoa Genotypes in Response to Salt Stress

iTRAQ-Based Comparative Proteomic Analysis of Seedling Leaves of Two Upland Cotton Genotypes Differing in Salt Tolerance

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