Overexpression of a specific OsGSTL2 isoenzyme improves glyphosate and chlorsulfuron tolerance of transgenic rice plants

Hu, Tingzhang; Jiao, Yang; Wu, Xiaoli; Huang, Xiaoyun; Chen, Zaigang; Wu, Yingmei

doi:10.5897/ajar12.868

Afr. J. Agric. Res.

2013

DOI: 10.5897/ajar12.868

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Overexpression of a specific OsGSTL2 isoenzyme improves glyphosate and chlorsulfuron tolerance of transgenic rice plants

Tingzhang Hu¹,

Yang Jiao²,

Xiaoli Wu³

et al.

Abstract: Plant glutathione S-transferases (GSTs

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Cited by 3 publications

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Proteomic investigation of Zn-challenged rice roots reveals adverse effects and root physiological adaptation

et al. 2020

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Aims Understanding the molecular responses of plant roots to the challenging environment contributes to engineering plants with improved stress tolerance. However, little has been done to understand rice (Oryza sativa L.) roots response to the toxic concentration of zinc (Zn) at the proteomic level. This study explored proteomic responses of young rice roots 5-6 days after sowing to 765 µM of Zn in a hydroponic set-up after 4-day exposure.Methods Dye staining method and spectrophotometry were chosen for physiological response investigations. ICP-MS was used to determine metal content in rice seedlings. Two-dimensional gel electrophoresis was used for the proteomic study of root tissue.Results Elevated Zn reduced Mg translocation to the shoots and Mn uptake, decreased plant growth, and increased cell death of roots. Zn stimulated the biosynthesis of glutathione but decreased ROS and malondialdehyde levels. In total, 42 Zn-responsive proteins were identified. Proteins involved in redox regulation, defense response, sulfur metabolism, and proteolysis were induced, while those of energy production and cell wall biogenesis were decreased.Conclusion Roots of O. sativa seedlings could tolerate certain Zn excess (765 µM Zn for 4 days) by stimulating nutrient recycling and glycolysis, and production of defensive metabolites and proteins to maintain ion and redox homeostasis. Besides, adenosylhomocysteinase (AHCY) down-regulation may contribute to balanced transmethylations, and methionine salvage pathway appears important for the adaptation to Zn. Our results provide some new insight into the complex metabolic response of rice roots to Zn stress, which is related to both stress and defense mechanisms.

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Proteomic investigation of Zn-challenged rice roots reveals adverse effects and root physiological adaptation

et al. 2020

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“Plant Glutathione S-transferases: An overview”

Estévez

Hernández

2020

Plant Gene

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Multiple Metabolic Enzymes Can Be Involved in Cross-Resistance to 4-Hydroxyphenylpyruvate-Dioxygenase-Inhibiting Herbicides in Wild Radish

Liu

et al. 2023

J. Agric. Food Chem.

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A wild radish population (R) has been recently confirmed to be cross-resistant to 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicides without previous exposure to these herbicides. This cross-resistance is endowed by enhanced metabolism. Our study identified one 2-oxoglutarate/Fe(II)-dependent dioxygenase gene (Rr2ODD1) and two P450 genes (RrCYP704C1 and RrCYP709B1), which were significantly more highly expressed in R versus susceptible (S) plants. Gene functional characterization using Arabidopsis transformation showed that overexpression of RrCYP709B1 conferred a modest level of resistance to mesotrione. Ultra-performance liquid chromatography–tandem mass spectrometry analysis showed that tissue mesotrione levels in RrCYP709B1 transgenic Arabidopsis plants were significantly lower than that in the wild type. In addition, overexpression of Rr2ODD1 or RrCYP704C1 in Arabidopsis endowed resistance to tembotrione and isoxaflutole. Structural modeling indicated that mesotrione can bind to CYP709B1 and be easily hydroxylated to form 4-OH-mesotrione. Although each gene confers a modest level of resistance, overexpression of the multiple herbicide-metabolizing genes could contribute to HPPD-inhibiting herbicide resistance in this wild radish population.

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Overexpression of a specific OsGSTL2 isoenzyme improves glyphosate and chlorsulfuron tolerance of transgenic rice plants

Abstract: Plant glutathione S-transferases (GSTs

Cited by 3 publications

References 53 publications

Proteomic investigation of Zn-challenged rice roots reveals adverse effects and root physiological adaptation

Proteomic investigation of Zn-challenged rice roots reveals adverse effects and root physiological adaptation

“Plant Glutathione S-transferases: An overview”

Multiple Metabolic Enzymes Can Be Involved in Cross-Resistance to 4-Hydroxyphenylpyruvate-Dioxygenase-Inhibiting Herbicides in Wild Radish

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