Overexpression of Acyl-ACP Thioesterases, CpFatB4 and CpFatB5, Induce Distinct Gene Expression Reprogramming in Developing Seeds of Brassica napus

Nam, Jeong-Won; Yeon, Jinouk; Jeong, Jiseong; Cho, Eunyoung; Kim, Ho Bang; Hur, Yoonkang; Lee, Kyeong‐Ryeol; Yi, Hankuil

doi:10.3390/ijms20133334

Cited by 4 publications

(2 citation statements)

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“…Whereas, except for specific medium-chain fatty acyl-ACP thioesterase (Fat) B, which was firstly isolated from Umbellulana californica (UcFatB1) [ 32 , 33 ], the biosynthetic pathway of MCT is covered in the laurel family . UcFatB1 and its orthologs are ubiquitous in MCT-producing plants and are acknowledged as key regulators to determine chain length of medium-chain fatty acid (MCFA) [ 34 , 35 ]. In an in vitro assay, UcFatB1 was observed to hydrolyze the growing acyl thioesters prematurely to yield C12:0 [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

Chromosome-level genome assembly and resequencing of camphor tree (Cinnamomum camphora) provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of Cinnamomum

Wang

Zheng

et al. 2022

Horticulture Research

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Cinnamomum species attract attentions owing to their scents, medicinal properties, and ambiguous relationship in the phylogenetic tree. Here, we report a high-quality genome assembly of C. camphora, based on which two whole-genome duplication (WGD) events were detected in the C. camphora genome: one was shared with Magnoliales, and the other was unique to Lauraceae. Phylogenetic analyses illustrated that Lauraceae species formed a compact sister clade to the eudicots. We then performed whole-genome resequencing on 24 Cinnamomum species native to China, and the results showed that the topology of Cinnamomum species was not entirely consistent with morphological classification. The rise and molecular basis of chemodiversity in Cinnamomum were also fascinating issues. In this study, six chemotypes were classified and six main terpenoids were identified as major contributors of chemodiversity in C. camphora by the principal component analysis. Through in vitro assays and subcellular localization analyses, we identified two key terpene synthase (TPS) genes (CcTPS16 and CcTPS54), the products of which were characterized to catalyze the biosynthesis of two uppermost volatiles (i.e.,1,8-cineole and (iso)nerolidol), respectively, and meditate the generation of two chemotypes by transcriptional regulation and compartmentalization. Additionally, the pathway of medium-chain triglyceride (MCT) biosynthesis in Lauraceae was investigated for the first time. Synteny analysis suggested that the divergent synthesis of MCT and long-chain triglyceride (LCT) in Lauraceae kernels was probably controlled by specific medium-chain fatty acyl-ACP thioesterase (FatB), type-B lysophosphatidic acid acyltransferase (type-B LPAAT), and diacylglycerol acyltransferase 2b (DGAT 2b) isoforms during co-evolution with retentions or deletions in the genome.

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Section: Introductionmentioning

confidence: 99%

Chromosome-level genome assembly and resequencing of camphor tree (Cinnamomum camphora) provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of Cinnamomum

Wang

Zheng

et al. 2022

Horticulture Research

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“…They also have the potential to be hydrocarbon components in kerosene-type jet fuel along with the 16:0 fatty acid (palmitic acid) (Kim et al 2015). To date, dozens of acyl-ACP thioesterases, which were isolated from seeds that accumulate high levels of MCFAs, have been functionally characterized (Beermann et al 2007;Dehesh et al 1996a, b;Filichkin et al 2006;Jing et al 2011;Jones et al 1995;Kim et al 2015;Leonard et al 1997;Nam et al 2019;Tjellström et al 2013;Töpfer 1995;Voelker et al 1992). For example, UcFatB cDNA from Umbellularia californica (California bay) seeds has been shown to encode a 12:0-ACP thioesterase and produces lauric acid in the seeds when expressed in Arabidopsis thaliana (Arabidopsis) and Brassica napus (rapeseed) (Davies et al 199;Knutzon et al 1999;Pollard et al 1991;Voelker et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis

et al. 2019

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Cuphea viscosissima plants accumulate mediumchain fatty acids (MCFAs), i.e., those containing 8~14 carbons, in their seeds, in addition to the longer carbon chain fatty acids (≥16 carbons) found in a variety of plant species. Previous studies have reported the existence of three C. viscosissima MCFA-producing acyl-acyl carrier protein (ACP) thioesterases with different substrate specificities. In this study, CvFatB4, a novel cDNA clone encoding an acyl-ACP thioesterase (EC 3.1.2.14), was isolated from developing C. viscosissima seeds. Sequence alignment of the deduced amino acid sequence revealed that four catalytic residues for thioesterase activity are conserved and a putative N-terminal chloroplast transit peptide is present. Overexpression of CvFatB4 cDNA, which was under the control of the cauliflower mosaic virus 35S promoter, in Arabidopsis thaliana led to an increase in 16:0 fatty acid (palmitate) levels in the seed oil at the expense of 18:1 and other non-MCFAs.

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Genetic variation and QTL analysis of saturated fatty acids in two doubled haploid populations of oilseed rape (Brassica napus L.)

2022

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Consumption of foodstuff with low contents of saturated fatty acids is considered beneficial for human health. Reducing saturated fatty acid content in oilseed rape (canola) and other oil and protein crops is a relevant breeding aim. The objective of this work was to study the genetic variation and inheritance of saturated fatty acids in two DH populations of oilseed rape, to map QTL and to identify candidate genes. In addition, the correlation to other seed quality traits was studied. To this end, two half-sib DH populations were tested in up to five field environments in north-western Europe and seeds harvested from open-pollinated seeds were analyzed. Genotyping was performed using Illumina Brassica 15 K SNP chip. In both populations, significant effects for the genotypes and for the environments were detected, and heritability ranged from 68 to 89% for the predominant palmitic acid and stearic acid content. Up to 48 QTL for different fatty acids, oil and acid detergent lignin (ADL) content were mapped in the two populations. Co-locating QTL for palmitic acid, stearic acid, the C16/18 fatty acid ratio, the FATB/A ratio, oil and ADL content were identified on different chromosomes. A large number of candidate genes were identified within the vicinity of QTL flanking markers. Identification of several co-locating QTL positions, of associated candidate genes and SNP markers should facilitate oilseed rape breeding for low saturated fatty acid content.

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Overexpression of Acyl-ACP Thioesterases, CpFatB4 and CpFatB5, Induce Distinct Gene Expression Reprogramming in Developing Seeds of Brassica napus

Cited by 4 publications

References 44 publications

Chromosome-level genome assembly and resequencing of camphor tree (Cinnamomum camphora) provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of Cinnamomum

Chromosome-level genome assembly and resequencing of camphor tree (Cinnamomum camphora) provides insight into phylogeny and diversification of terpenoid and triglyceride biosynthesis of Cinnamomum

Overexpression of Cuphea viscosissima CvFatB4 enhances 16:0 fatty acid accumulation in Arabidopsis

Genetic variation and QTL analysis of saturated fatty acids in two doubled haploid populations of oilseed rape (Brassica napus L.)

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