Overexpression of an aquaporin protein from Aspergillus glaucus confers salt tolerance in transgenic soybean

Li, Feiwu; Ni, Hejia; Wang, Yan; Xie, Yanbo; Liu, Xiaodan; Tan, Xiaoming; Zhang, Ling; Zhang, Shihong

doi:10.1007/s11248-021-00280-9

Cited by 8 publications

(5 citation statements)

References 47 publications

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“…The AQP gene expression was induced by salt stress in various crops [54][55][56]. Overexpression of the AQP gene enhanced salt tolerance of wheat [57], soybean [13], and banana [58]. PIP is also involved in the loss of water from plant cells and up-regulating expression of PIP may decrease salt and drought stress tolerance [59].…”

Section: Amf Inoculation Enhanced the Tolerance Of Citrus To Salt Str...mentioning

confidence: 99%

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Effects of Interaction of Protein Hydrolysate and Arbuscular Mycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress

Lu,

Jin,

Wang

et al. 2023

JoF

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Protein hydrolysates (PHs) and arbuscular mycorrhizal fungi (AMF) are environmentally friendly biostimulants that effectively promote crop growth and alleviate the damage from abiotic stress. However, the physiological and molecular regulatory mechanisms are still unclear. This study aimed to explore the effects of PHs and AMF on growth, mineral nutrient absorption, and expression of Aquaporins and SOSs in Goutoucheng (Citrus aurantium) under salt stress. Results showed that PH application and AMF inoculation significantly promoted plant growth and enhanced mineral element absorption and sodium effluxion in citrus under salt stress. The biomass, root activity, leaves mineral nutrition contents in PHs, AMF, and combined (PHs and AMF) treatments were significantly higher than those of control. Leaves sodium content in three treatments was significantly lower than in the control. AMF and combined treatments showed dominant effects than PHs alone. Besides, PHs interacted with AMF on growth, nutrient absorption, and sodium effluxion. Importantly, AMF and PHs induced stress-responsive genes. PIP1, PIP3, SOS1, and SOS3 expression in PHs and AMF treatments was significantly higher than control. Thus, it was concluded that AMF and PHs enhanced the salt tolerance of citrus by promoting nutrient absorption and sodium effluxion via up-regulating the expression of PIPs and SOSs. The mixed application of PHs and AMF had a better effect.

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Section: Amf Inoculation Enhanced the Tolerance Of Citrus To Salt Str...mentioning

confidence: 99%

“…Aquaporins (AQPs), especially plasma membrane intrinsic protein genes (PIPs) and tonoplast intrinsic protein genes (TIPs), play critical roles in regulating water uptake by crop roots [11]. Moreover, overexpression of AQP proteins imparts salt tolerance in transgenic plants through enhancing water acquisition [12,13].…”

Section: Introductionmentioning

confidence: 99%

Effects of Interaction of Protein Hydrolysate and Arbuscular Mycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress

Lu,

Jin,

Wang

et al. 2023

JoF

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“…The full-length protein sequence of Glyma.05G240500 from G. max, GlysoPI483463.05G206500 from G. soja, Vigun03g010300 from Vigna unguiculata, Phvul.002G322700 from Phaseolus vulgaris, Lj4g0019304 from Lotus japonicus, Ca_10406 from Cicer arietinum, Tp57577_TGAC_v2_mRNA19939 from Trifolium pratense, Medtr8g105780 from Medicago truncatula, arahy.Tifrunner.gnm1.ann1.B3N TUN from Arachis hypogaea, Lalb_Chr16g0387451 from Lupinus albus, MD13G1018800 from Malus domestica, Caden.03G117800 from Castanea dentata, Prupe.1G335100 from Prunus persica, Potri.010G104300 from Populus trichocarpa, and Thecc.02G308200 from Theobroma cacao were downloaded and aligned to create a phylogenetic tree based on the neighbor-joining method with the default parameter value using MEGA 5.0 software [29,30].…”

Section: Phylogenetic Analysis Of the Candidate Genementioning

confidence: 99%

QTL Mapping and Functional Identification of Candidate Genes Regulated by Sinorhizobium fredii HH103 and Associated with Nodulation Traits in Soybean

Ni,

Tian,

Zhang

et al. 2023

Agronomy

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Large amounts of nitrogen fertilizer are annually applied to improve soybean yield. However, the overuse of nitrogen fertilizers has resulted in significant environmental pollution. Biological nitrogen fixation is an ecological and environmentally friendly method to increase soybean yield. However, the signaling pathway and function of genes in the plant host regulated by rhizobia under the symbiotic relationship remain unknown. In this study, the chromosome region in soybean responsive to Sinorhizobium fredii HH103 was identified using chromosome-segment-substituted lines produced from a cross between cultivated soybean SN14 and wild soybean Zyd00006. In addition, candidate genes associated with nodulation traits and regulated by S. fredii HH103 were identified. In total, three quantitative trait loci (QTLs) containing 68 genes were identified on chromosomes 02, 05, and 20. The differentially expressed genes among the QTL regions were determined using RNA-seq and qRT-PCR. Glyma. 05G240500, a potential gene responding to S. fredii HH103 and positively regulating soybean nodulation, was identified. To explore the relationships between haplotypes and soybean nodulation phenotypes, SNPs found in the regulatory areas of Glyma.05G240500 haplotype were investigated. Our study revealed the role of Glyma.05G240500 in symbiosis and provided a reference for facilitating symbiotic nitrogen fixation in the field and for marker-assisted selection.

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“…Later, transgenic lines were subjected to salt stress conditions, and results showed that transgenic lines exhibited a significant tolerance to salt stress compared to the wild type. These results showed the stable transformation and expression of AgGlpF ( Table 5 ) in the soybean genome and its response to salt stress [ 90 ]. Ren et al [ 91 ] studied the role of the soybean salt-tolerant gene GmST1 ( Table 5 ) and concluded that this gene reduced ROS production and increased sensitivity to ABA under salt stress situations [ 91 ].…”

Section: Genetic Engineering For Salinity Tolerance In Soybeanmentioning

confidence: 99%

Molecular Tools and Their Applications in Developing Salt-Tolerant Soybean (Glycine max L.) Cultivars

et al. 2022

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Abiotic stresses are one of the significant threats to soybean (Glycine max L.) growth and yields worldwide. Soybean has a crucial role in the global food supply chain and food security and contributes the main protein share compared to other crops. Hence, there is a vast scientific saddle on soybean researchers to develop tolerant genotypes to meet the growing need of food for the huge population. A large portion of cultivated land is damaged by salinity stress, and the situation worsens yearly. In past years, many attempts have increased soybean resilience to salinity stress. Different molecular techniques such as quantitative trait loci mapping (QTL), genetic engineering, transcriptome, transcription factor analysis (TFs), CRISPR/Cas9, as well as other conventional methods are used for the breeding of salt-tolerant cultivars of soybean to safeguard its yield under changing environments. These powerful genetic tools ensure sustainable soybean yields, preserving genetic variability for future use. Only a few reports about a detailed overview of soybean salinity tolerance have been published. Therefore, this review focuses on a detailed overview of several molecular techniques for soybean salinity tolerance and draws a future research direction. Thus, the updated review will provide complete guidelines for researchers working on the genetic mechanism of salinity tolerance in soybean.

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Overexpression of an aquaporin protein from Aspergillus glaucus confers salt tolerance in transgenic soybean

Cited by 8 publications

References 47 publications

Effects of Interaction of Protein Hydrolysate and Arbuscular Mycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress

Effects of Interaction of Protein Hydrolysate and Arbuscular Mycorrhizal Fungi Effects on Citrus Growth and Expressions of Stress-Responsive Genes (Aquaporins and SOSs) under Salt Stress

QTL Mapping and Functional Identification of Candidate Genes Regulated by Sinorhizobium fredii HH103 and Associated with Nodulation Traits in Soybean

Molecular Tools and Their Applications in Developing Salt-Tolerant Soybean (Glycine max L.) Cultivars

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