Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco

Tian, Li; Sun, Jingkuan; Bi, Yuping; Peng, Zhenying

doi:10.1007/s00344-015-9565-y

Cited by 18 publications

(15 citation statements)

References 52 publications

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“…GO analyses showed 10 TFs investigated here belong to the category of salt stress response genes, in which 8/10 of genes are MYB TFs, such as Glyma.12G104800 , Glyma.16G073000 , Glyma.01G107500 , Glyma.15G236400 , Glyma.06G097100 . This is consistent with previous studies that MYB TFs have been known to regulate salt stress response in plants (Yang et al, 2012; Cui et al, 2013; Li et al, 2016; Wei et al, 2017). Notably, the fold changes of some TFs were significantly higher than control plants ( Supplementary Table 3 ).…”

Section: Resultssupporting

confidence: 94%

“…It has been reported that the REF6 protein, also known as JMJ12, can specifically demethylate H3K27me3 at its target gene loci to active gene expression (Lu et al, 2011). The REF6 mutants cause the ectopic accumulation of H3K27me3 at hundreds of genes and a number of defective developmental phenotypes (Yu et al, 2008; Cui et al, 2016; Li et al, 2016). ELF6 identified as an H3K27me2/3-specific demethylase closely related to REF6 was rquired for removal of H3K27me3 from the Flowering Locus C ( FLC ) locus in developing embryos in vernalized plants (Crevillen et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

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Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

et al. 2019

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Soybean is an important economic crop for human diet, animal feeds and biodiesel due to high protein and oil content. Its productivity is significantly hampered by salt stress, which impairs plant growth and development by affecting gene expression, in part, through epigenetic modification of chromatin status. However, little is known about epigenetic regulation of stress response in soybean roots. Here, we used RNA-seq and ChIP-seq technologies to study the dynamics of genome-wide transcription and histone methylation patterns in soybean roots under salt stress. Eight thousand seven hundred ninety eight soybean genes changed their expression under salt stress treatment. Whole-genome ChIP-seq study of an epigenetic repressive mark, histone H3 lysine 27 trimethylation (H3K27me3), revealed the changes in H3K27me3 deposition during the response to salt stress. Unexpectedly, we found that most of the inactivation of genes under salt stress is strongly correlated with the de novo establishment of H3K27me3 in various parts of the promoter or coding regions where there is no H3K27me3 in control plants. In addition, the soybean histone modifiers were identified which may contribute to de novo histone methylation and gene silencing under salt stress. Thus, dynamic chromatin regulation, switch between active and inactive modes, occur at target loci in order to respond to salt stress in soybean. Our analysis demonstrates histone methylation modifications are correlated with the activation or inactivation of salt-inducible genes in soybean roots.

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Section: Resultssupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

et al. 2019

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“…MYB-related genes are the second largest member of the MYB gene family. Genes from this subfamily have diverse functions and play an important role in plant growth and stress responses, such as cell morphogenesis, secondary metabolism, organ morphogenesis, chloroplast development, leaf senescence, response to phosphate starvation and tolerance to cold, drought and salt (Dubos et al, 2010;Li et al, 2016;Yin et al, 2017;Park et al, 2018;Sun et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Poplar R2R3-MYB Gene Family and Over-Expression of PsnMYB108 Confers Salt Tolerance in Transgenic Tobacco

et al. 2020

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The MYB, one of the largest transcription factor families in plants, is related to various biological processes. For an example, the R2R3-MYB family plays an important role in regulation of primary and secondary metabolism, plant growth and development, and responses to hormones and stresses. However, functional studies on the poplar R2R3-MYB genes are limited. In this study, we identified 207 poplar R2R3-MYB genes that are unevenly distributed on the 19 chromosomes of poplar, followed by characterization of their conserved domains. On the basis of phylogenetic analysis, these genes can be divided into 23 groups. Evidence from synteny analyses indicated that the poplar R2R3-MYB gene family is featured by tandem and segmental duplication events. On the basis of RNA-Seq data, we investigated salt responsive genes and explored their expression patterns. Furthermore, we cloned the PsnMYB108 gene from poplar, which is significantly up-regulated in roots and leaves in response to salt stress. To validate its function, we developed transgenic tobacco plants that over-express the PsnMYB108 gene. It appears that the transgenic lines are more tolerant to salt stress than the wild type does. Evidence from physiological analyses demonstrated that over-expression of PsnMYB108 may improve tobacco salt stress tolerance by increasing the reactive oxygen species scavenging ability and the accumulation of proline. These results laid the foundation for future analysis and functional studies of poplar R2R3-MYB family members, and revealed that PsnMYB108 plays an important role in improving plant salt stress tolerance.

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“…In addition, it is reported that under stress, the expression levels NtDREB, NtRD29A (response to dehydration), NtERD10C were significantly up-regulated in transgenic tobacco of a FvMYB1 gene from Fraxinus velutina (Li et al, 2016). The increased gene expressions of stress-related genes in ZmNAGK transgenic tobaccos indicated there are different targets downstream NO to improve drought stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

et al. 2019

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Water deficit is a key limiting factor that affects the growth, development and productivity of crops. It is vital to understand the mechanisms by which plants respond to drought stress. Here an N-acetylglutamate kinase gene, ZmNAGK, was cloned from maize (Zea mays). ZmNAGK was expressed at high levels in maize leaves and at lower levels in root, stem, female flower and male flower. The expression of ZmNAGK was significantly induced by PEG, NaCl, ABA, brassinosteroid and H2O2. The ectopic expression of ZmNAGK in tobacco resulted in higher tolerance to drought compared to plants transformed with empty vector. Further physiological analysis revealed that overexpression of ZmNAGK could enhance the activities of antioxidant defense enzymes, and decrease malondialdehyde content and leakage of electrolyte in tobacco under drought stress. Moreover, the ZmNAGK transgenic tobacco accumulated more arginine and nitric oxide (NO) than control plants under drought stress. In addition, the ZmNAGK transgenic tobaccos activated drought responses faster than vector-transformed plants. These results indicate that ZmNAGK can play a vital role in enhancing drought tolerance by likely affecting the arginine and NO accumulation, and ZmNAGK could be involved in different strategies in response to drought stress.

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Overexpression of an MYB-Related Gene FvMYB1 from Fraxinus velutina Increases Tolerance to Salt Stress in Transgenic Tobacco

Cited by 18 publications

References 52 publications

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

Dynamic Changes in Genome-Wide Histone3 Lysine27 Trimethylation and Gene Expression of Soybean Roots in Response to Salt Stress

Characterization of the Poplar R2R3-MYB Gene Family and Over-Expression of PsnMYB108 Confers Salt Tolerance in Transgenic Tobacco

Over-Expression of a Maize N-Acetylglutamate Kinase Gene (ZmNAGK) Improves Drought Tolerance in Tobacco

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