Overexpression of Arabidopsis damaged DNA binding protein 1A (DDB1A) enhances UV tolerance

Abstract

Damaged DNA Binding protein 1 (DDB1) is a conserved protein and a component of multiple cellular complexes. Arabidopsis has two homologues of DDB1: DDB1A and DDB1B. In this study we examine the role of DDB1A in Arabidopsis UV tolerance and DNA repair using a DDB1A null mutant (ddb1a) and overexpression lines. DDB1A overexpression lines showed higher levels of UV-resistance than wild-type in a range of assays as well as faster DNA repair. However a significant difference between wild-type plants and ddb1a mutants was only observed immediately following UV treatment in root length and photoproduct repair assays. DDB1A and DDB1B mRNA levels increased 3 h after UV exposure and DDB1A is required for UV regulation of DDB1B and DDB2 mRNA levels. In conclusion, while DDB1A is sufficient to increase Arabidopsis UV tolerance, it is only necessary for immediate response to UV damage.

Thiourea mediates alleviation of UV-B stress-induced damage in the Indian mustard (Brassica junceaL.)

“…Even though success has been obtained in some cases (Jayaraj and Punja 2008;Al Khateeb and Schroeder 2009), to date there are no transgenic crop plants shown to perform successfully at the field level. In our previous research, three different sulfhydryl compounds (ÁSH containing), namely thiourea (TU), dithiothreitol (DTT) and thioglycolic acid (TGA) were chosen, because of their ability to maintain the redox state, to assess their effects on the performance of wheat and mustard crops under multistress conditions (mainly heat and drought stress) of Rajasthan, India.…”

Arabidopsis Cockayne Syndrome A-Like Proteins 1A and 1B Form a Complex with CULLIN4 and Damage DNA Binding Protein 1A and Regulate the Response to UV Irradiation

“…It has been shown that an Arabidopsis mutant lacking DDB2 has enhanced sensitivity to UV irradiation, methyl methanesulfonate (MMS), and H 2 O 2 stress (Koga et al, 2006) and that overexpression of Arabidopsis DDB1A increases plant UV tolerance (Al Khateeb and Schroeder, 2009). Moreover, DDB2 associates with the CUL4-DDB1A complex to form an E3 ligase that modulates GGR-type DNA damage repair upon UV stress (Molinier et al, 2008).…”

Recognition and Repair Pathways of Damaged DNA in Higher Plants

“…For most of the other NER proteins that are conserved among animals and plants, a role in DNA repair has been demonstrated, frequently by reverse genetic studies in Arabidopsis thaliana. Here, proteins shown to be involved in damaged DNA recognition in animals, such as DCX-E3 ligases, DDB2 and CSA, have also been recently described by several groups in plants (Bernhardt et al, 2006;Molinier et al, 2008;Al Khateeb and Schroeder, 2009;Bernhardt et al, 2010;Biedermann and Hellmann, 2010;Zhang and Schroeder, 2010;Castells et al, 2011). While plants affected in ATCSA-1, the Arabidopsis CSA ortholog, do not display an abnormal development (Biedermann and Hellmann, 2010), loss of CUL4 or DDB2 cause a dwarf-like phenotype (Bernhardt et al, 2006;Koga et al, 2006).…”