Damaged DNA Binding protein 1 (DDB1) is a conserved protein and a component of multiple cellular complexes. Arabidopsis has two homologues of DDB1: DDB1A and DDB1B. In this study we examine the role of DDB1A in Arabidopsis UV tolerance and DNA repair using a DDB1A null mutant (ddb1a) and overexpression lines. DDB1A overexpression lines showed higher levels of UV-resistance than wild-type in a range of assays as well as faster DNA repair. However a significant difference between wild-type plants and ddb1a mutants was only observed immediately following UV treatment in root length and photoproduct repair assays. DDB1A and DDB1B mRNA levels increased 3 h after UV exposure and DDB1A is required for UV regulation of DDB1B and DDB2 mRNA levels. In conclusion, while DDB1A is sufficient to increase Arabidopsis UV tolerance, it is only necessary for immediate response to UV damage.

Damaged DNA-binding proteins 1 and 2 (DDB1 and DDB2) are subunits of the damaged DNA-binding protein complex (DDB). DDB1 is also found in the same complex as DE-ETIOLATED 1 (DET1), a negative regulator of light-mediated responses in plants. Arabidopsis has two DDB1 homologs, DDB1A and DDB1B. ddb1a single mutants have no visible phenotype while ddb1b mutants are lethal. We have identified a partial loss-of-function allele of DDB2. To understand the genetic interaction among DDB2, DDB1A, and DET1 during Arabidopsis light signaling, we generated single, double, and triple mutants. det1 ddb2 partially enhances the short hypocotyl and suppresses the high anthocyanin content of dark-grown det1 and suppresses the low chlorophyll content, early flowering time (days), and small rosette diameter of light-grown det1. No significant differences were observed between det1 ddb1a and det1 ddb1a ddb2 in rosette diameter, dark hypocotyl length, and anthocyanin content, suggesting that these are DDB1A-dependent phenotypes. In contrast, det1 ddb1a ddb2 showed higher chlorophyll content and later flowering time than det1 ddb1a, indicating that these are DDB1A-independent phenotypes. We propose that the DDB1A-dependent phenotypes indicate a competition between DDB2-and DET1-containing complexes for available DDB1A, while, for DDB1A-independent phenotypes, DDB1B is able to fulfill this role. P LANT development is dependent on environmental conditions. Because light is the energy source for plant growth, plants have evolved highly sensitive mechanisms for perceiving light. This information is used to regulate development and to maximize light utilization for photosynthesis. The transition from the vegetative to the reproductive stage is also regulated by light. Seedlings implement different developmental programs when grown in light or darkness. Light-grown seedlings undergo photomorphogenesis, exhibiting short hypocotyls, open and expanded cotyledons, and photosynthetically active chloroplasts. In contrast, seedlings grown under dark conditions are etiolated, having closed and unexpanded cotyledons, elongated hypocotyls, and undeveloped chloroplasts. This developmental pattern is known as skotomorphogenesis (Chen et al. 2004).This developmental switch (etiolation/de-etiolation) is under the control of at least 10 genes (COP/DET/ FUS). Molecular genetic studies in Arabidopsis indicate that these proteins function downstream of the photoreceptors to repress photomorphogenesis in the absence of light. Mutation of these genes results in seedlings with a de-etiolated (det) or constitutive photomorphogenic (cop) phenotype when grown under dark conditions. The null mutations of these genes are seedling lethal with high anthocyanin levels ( fus) (Wang and Deng 2002). COP1 is a WD-40 and RING finger protein with E3 ubiquitin (Ub) ligase activity, which targets photoreceptors and downstream transcription factors for ubiquitination and subsequent degradation. The COP9 signalosome (CSN) is a multiprotein complex composed of eight subunit...

This paper focuses on characterizing the current status of physiochemical properties of Mujib Dam sediments. Five types of granulometric textural facies were observed for the bottom sediments of Mujib reservoir bed; these are clayey facies, clayey-silt facies, sand-silt-clay facies, sand facies, and granule facies. This average grain size will likely play a vital role in adsorption-desorption of the majority trace metals to the reservoir lake. Other sediment parameters including the total averages were 5.9% (total organic matter (TOM)), 7.5 (pH), 25.8% (CaCO), and 88.0 meq/100 g (cation exchange capacity), with dominant mineralogical constituents of quartz, calcite, dolomite, and minor feldspar and with variability in clay mineral types. The vast majority of trace metals in sediment exhibited values in the range or near the upper limit of the normal worldwide soil ranges. TOM and grain size of sediment are major factors governing the trace metal concentrations. The calculated geoaccumulation index (I ) and enrichment factor (EF) of metals in sediments of Mujib Dam were ranked as follows: cadmium (Cd)> copper (Cu) > zinc (Zn) > lead (Pb) > cobalt (Co) > iron (Fe) > chromium (Cr) > nickel (Ni) > manganese (Mn) > Sr based on the I and Cd> Zn > Pb > Co > Cr > Cu > Sr > Ni > Mn according to the EF values. The estimated percentage loss in volumetric capacity of the reservoir due to sedimentation was 1.55% per year, indicating that the sediment currently occupied 18.63% of the original reservoir storage capacity. The maximum life span of reservoir is about 64.46 years.

Plant DNA is damaged by exposure to solar radiation, which includes ultraviolet (UV) rays. UV damaged DNA is repaired either by photolyases, using visible light energy, or by nucleotide excision repair (NER), also known as dark repair. NER consists of two subpathways: global genomic repair (GGR), which repairs untranscribed DNA throughout the genome, and transcription-coupled repair (TCR), which repairs transcribed DNA. In mammals, CSA, CSB, UVSSA, USP7, and TFIIS have been implicated in TCR. Arabidopsis homologs of CSA (AtCSA-1/2) and CSB (CHR8) have previously been shown to contribute to UV tolerance. Here we examine the role of Arabidopsis homologs of UVSSA, USP7 (UBP12/13), and TFIIS (RDO2) in UV tolerance. We find that loss of function alleles of UVSSA, UBP12 , and RDO2 exhibit increased UV sensitivity in both seedlings and adults. UV sensitivity in atcsa-1, uvssa , and ubp12 mutants is specific to dark conditions, consistent with a role in NER. Interestingly, chr8 mutants exhibit UV sensitivity in both light and dark conditions, suggesting that the Arabidopsis CSB homolog may play a role in both NER and light repair. Overall our results indicate a conserved role for UVSSA, USP7 (UBP12), and TFIIS (RDO2) in TCR.