Overexpression of C10orf116 promotes proliferation, inhibits apoptosis and enhances glucose transport in 3T3-L1 adipocytes

Abstract

Abstract. Data from our previous study demonstrated thatC10orf116 is an adipocyte lineage-specific nuclear factor, which regulates master adipogenesis transcription factors during early differentiation. However, the precise functional properties of this gene have yet to be identified and further investigation is required. In the present study, we report the effects of C10orf116 expression on cell proliferation and apoptosis in vitro and observed that the overexpression of C10orf116 stimulates proliferation and inhibits apoptosis in preadipocytes. Furthermore, we investigated the effects of C10orf116 on glucose uptake and demonstrated that the ectopic expression of C10orf116 significantly increases insulin-stimulated glucose uptake in adipocytes by increasing glucose transporter type 4 (GLUT4) expression levels. Collectively, these data further support the hypothesis that C10orf116 is important in regulating glucose transport in adipocytes as well as the number of preadipocytes. The results of the present study may also provide insights into the complex mechanisms involved in the development of obesity.

Population effect model identifies gene expression predictors of survival outcomes in lung adenocarcinoma for both Caucasian and Asian patients

“…However, the expression for several genes, such as GDF10 , C10orf116 , GCOM1 , GART , WDR46 , SLC25A10 and PECAM1 were population specific, in which C10orf116 [24], GART [25] and SLC25A10 [26] had been reported to be functional in metabolic processes. These results were consistent with the previous findings that Asians and Caucasians had significantly different metabolic profiles [27].…”

“…This indicates that for lung adenocarcinoma patients from both Asian and Caucasian cohort studies, the apoptosis pathway might be turned down and cell-cycle pathway might be powered up. Also, we observed that metabolic genes including C10orf116 [24], GART [25] and SLC25A10 were differentially expressed between Caucasian and Asian populations. However, additional studies are required to validate that these discrepancies are population-specific and not due to technical variation.…”

A Comprehensive Analysis of SE-lncRNA/mRNA Differential Expression Profiles During Chondrogenic Differentiation of Human Bone Marrow Mesenchymal Stem Cells

“…Overexpression of ADIRF has been shown to increase the transcription of C/EBPα and PPARγ to facilitate adipogenic differentiation. Overexpression of ADIRF also promotes cell proliferation and inhibits apoptosis ( Chen et al, 2013 ). Most of these mRNAs we screened out are involved in cytoskeletal changes, which have been proven to be closely linked to MSC differentiation ( Viale-Bouroncle et al, 2012 ).…”

Transcriptional profiling of chronic clinical hepatic schistosomiasis japonica indicates reduced metabolism and immune responses

“…The most downregulated gene in [S] was the uncharacterized C10orf116 (−6·0-fold) followed by GPAM (glycerol-3-phosphate acyltransferase, mitochondrial) at −5·2-fold. C10orf116, also known as Adipose most abundant gene transcript 2 (APM2), is known to be expressed in adipose tissue and over expression of the encoded protein leads to increased cellular proliferation and increased glucose transport but its full functional significance, particularly in the liver, is unknown (Chen et al 2013). GPAM, associated with many aspects of fatty acid metabolism and the synthesis of glycerolipids (Wendel et al 2009), was also downregulated in [SH] (−7·20-fold).…”