Overexpression of cell-cycle related and expression-elevated protein in tumor (CREPT) in malignant cervical cancer

Abstract

Objective: To explore the expression of cell-cycle related and expression-elevated protein in tumor (CREPT), cyclin D1, and transcription factor 4 (TCF4) in patients with cervical carcinoma. Methods: This study enrolled 20 patients with cervical cancer and 10 control patients diagnosed with benign cervical lesions undergoing resection at the People's Liberation Army General Hospital from January 2016 to December 2017. Cervical tissues were collected from all patients and their clinical characteristics were recorded. Protein and mRNA levels of CREPT, cyclin D1, and TCF4 were measured in tissue samples by immunohistochemistry, western blotting, and realtime polymerase chain reaction (PCR) and compared between the two groups. Result: Protein and mRNA expression levels of CREPT, cyclin D1, and TCF4 were all significantly higher in the cervical cancer compared with the control group, according to western blot and PCR, respectively. CREPT expression was also significantly higher in the cervical cancer group according to immunohistochemistry. Conclusion: Levels of CREPT, cyclin D1, and TCF4 were significantly elevated in cervical carcinoma tissues, and their expression levels were positively correlated, suggesting that these factors might play important roles during the development of cervical carcinoma.

Purification and Analysis of the CREPT Antibody from Mouse Ascites

“…Through genetic analysis, CREPT, p15RS, and RTT103 evolved in the same progenitor cells and participated in cell cycle regulation. Previous studies have found that CREPT is involved in various regulations of transcription, cell cycle, tumorigenesis, and DNA repair [ 3 , 4 ]. CREPT is a cell cycle-related protein.…”

Adhesion G protein-coupled receptor G2 accelerates the proliferation of cancer cells by promoting the formation of CDK4/CCND1

“…The activation of CDK depends on the cyclins that are expressed in the different phases of the cell cycle (13). CDK and cyclins combine to form a cell cycle-dependent complex that regulates the progression of the cell cycle (14), which may eventually affect the formation and development of tumors (15).…”

An Important Role for RPRD1B in the Heat Shock Response

“…Interestingly, numerous studies have shown that RPRD1B is highly expressed in a majority of solid tumors, where it promotes cell proliferation, tumor growth and progression. Furthermore, its levels correlate with poor prognosis and overall survival ( Li et al, 2018 ; Liu et al, 2019 ; Lu et al, 2012 ; Ma et al, 2020 ; Ma et al, 2017 ; She et al, 2014 ; Wang et al, 2014b ; Wen et al, 2020 ; Yin et al, 2019 ; Zhang et al, 2018 ; Zheng et al, 2016 ). RPRD proteins can modulate CTD Serine 5 (Ser5) and Ser7 phosphorylation levels, likely through the recruitment of the phosphatase RPAP2 ( Ni et al, 2011 ; Ni et al, 2014 ).…”