Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Zhou, Yong; He, Peng; Xu, Yuping; Liu, Qiang; Yang, Yingui; Liu, Shiqiang

doi:10.1186/s13568-017-0483-1

Cited by 31 publications

(24 citation statements)

References 48 publications

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“…In this study, it was found that DHNs not only act as drought-resistant protein, but also interact with FK506 binding protein in the nucleus and acts as regulatory protein for drought tolerance by regulating the ABA responsive signaling pathway (Tiwari et al, 2019). DHNs have been recognized as molecular protectors for functionality of some proteins such as lactate dehydrogenase and malate dehydrogenase in response to environmental stresses, as supported by previous studies (Zhou et al, 2017;Lv et al, 2018). Misfolding of proteins that are toxic to plants is triggered by drought stress, so protein quality control is critical to plant quality.…”

Section: Dhns and Other Proteins' Interactionssupporting

confidence: 64%

Dehydrins: an overview of current approaches and advancement

Güler¹,

Terzi²

2020

Turk J Bot

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Since plants are exposed to diverse environmental stresses in their natural area, numerous reviews have demonstrated many aspects of dehydrins (DHNs), including structural and functional dynamics, and multiple roles such as membrane protection, cryoprotection of enzymes, chaperone feature, and protection from reactive oxygen species. In this review, we have focused on new information, and other promising and emerging topics of DHNs in plants. This review outlines particularly the potential regulatory mechanisms of DHNs associated with stress tolerance and the role of DHNs in morphological responses of the plants exposed to environmental stresses. Besides the discussion of the signaling pathways involved in DHN expression under abiotic stress, novel aspects about the effect of DHN transcript or the protein accumulation on the tolerance to the stress factors are also presented in transgenic and non-transgenic plants. We hope that this review will help us better understand the role of these impressive proteins in plant stress response mechanisms.

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Section: Dhns and Other Proteins' Interactionssupporting

confidence: 64%

Dehydrins: an overview of current approaches and advancement

Güler¹,

Terzi²

2020

Turk J Bot

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“…In addition, this phenomenon is also associated with variation in temperature [ 43 ]. Lactate dehydrogenase (LDH) is widely used in dehydrin cryoprotective assays [ 7 , 9 , 16 , 44 , 45 ]. It has been reported that K2 and YSK2 dehydrin were able to protect LDH activity better than BSA.…”

Section: Molecular Structure Of Dehydrinsmentioning

confidence: 99%

“…Evidence from many studies has revealed a strong association between dehydrins and plant tolerance to heat stress. For instance, dehydrin CsLEA11 and WZY2 proteins were able to protect both recombinant E. coli and activity of the LDH enzyme under heat stress [ 9 , 16 ]. Dehydrin DHN-5 from wheat was found to play a relevant role in protecting enzyme β-glucosidase (bglG) against heat stress.…”

Section: The Regulation Of Dehydrin Genes Under Abiotic Stressesmentioning

confidence: 99%

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Structural and Functional Dynamics of Dehydrins: A Plant Protector Protein under Abiotic Stress

Wang

Zhang

2018

IJMS

117

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Abiotic stress affects the growth and development of crops tremendously, worldwide. To avoid adverse environmental effects, plants have evolved various efficient mechanisms to respond and adapt to harsh environmental factors. Stress conditions are associated with coordinated changes in gene expressions at a transcriptional level. Dehydrins have been extensively studied as protectors in plant cells, owing to their vital roles in sustaining the integrity of membranes and lactate dehydrogenase (LDH). Dehydrins are highly hydrophilic and thermostable intrinsically disordered proteins (IDPs), with at least one Lys-rich K-segment. Many dehydrins are induced by multiple stress factors, such as drought, salt, extreme temperatures, etc. This article reviews the role of dehydrins under abiotic stress, regulatory networks of dehydrin genes, and the physiological functions of dehydrins. Advances in our understanding of dehydrin structures, gene regulation and their close relationships with abiotic stresses demonstrates their remarkable ability to enhance stress tolerance in plants.

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“…Another study conducted on the hardy plant Prunus mume showed that five dehydrin genes from P. mume ( PmLEA8 , PmLEA10 , PmLEA19 , PmLEA20 , and PmLEA29 ) could enhance E. coli tolerance to salt and sorbitol stresses ( Bao et al, 2017 ). A Y 3 SK 2 -type dehydrin gene from cucumber ( Cucumis sativus ), CsLEA11 , could also enhance tolerance to high or low temperature when overexpressed in E. coli ( Zhou et al, 2017 ). Our research demonstrated that GST-IpDHN can be easily and obviously induced in E. coli BL21 ( Figure 3A ), and the spot assay, single clone counting assay, and cell growth curves of E. coli showed that IpDHN could significantly enhance salt/drought and sorbitol tolerance of E. coli cells ( Figure 3B and Supplementary Figures S4A,B ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Cloning and Functional Characterization of the Dehydrin (IpDHN) Gene From Ipomoea pes-caprae

Zhang

Zheng

et al. 2018

Front. Plant Sci.

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Dehydrin (DHN) genes can be rapidly induced to offset water deficit stresses in plants. Here, we reported on a dehydrin gene (IpDHN) related to salt tolerance isolated from Ipomoea pes-caprae L. (Convolvulaceae). The IpDHN protein shares a relatively high homology with Arabidopsis dehydrin ERD14 (At1g76180). IpDHN was shown to have a cytoplasmic localization pattern. Quantitative RT-PCR analyses indicated that IpDHN was differentially expressed in most organs of I. pes-caprae plants, and its expression level increased after salt, osmotic stress, oxidative stress, cold stress and ABA treatments. Analysis of the 974-bp promoter of IpDHN identified distinct cis-acting regulatory elements, including an MYB binding site (MBS), ABRE (ABA responding)-elements, Skn-1 motif, and TC-rich repeats. The induced expression of IpDHN in Escherichia coli indicated that IpDHN might be involved in salt, drought, osmotic, and oxidative stresses. We also generated transgenic Arabidopsis lines that over-expressed IpDHN. The transgenic Arabidopsis plants showed a significant enhancement in tolerance to salt/drought stresses, as well as less accumulation of hydrogen peroxide (H2O2) and the superoxide radical (O2−), accompanied by increasing activity of the antioxidant enzyme system in vivo. Under osmotic stresses, the overexpression of IpDHN in Arabidopsis can elevate the expression of ROS-related and stress-responsive genes and can improve the ROS-scavenging ability. Our results indicated that IpDHN is involved in cellular responses to salt and drought through a series of pleiotropic effects that are likely involved in ROS scavenging and therefore influence the physiological processes of microorganisms and plants exposed to many abiotic stresses.

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Overexpression of CsLEA11, a Y3SK2-type dehydrin gene from cucumber (Cucumis sativus), enhances tolerance to heat and cold in Escherichia coli

Cited by 31 publications

References 48 publications

Dehydrins: an overview of current approaches and advancement

Dehydrins: an overview of current approaches and advancement

Structural and Functional Dynamics of Dehydrins: A Plant Protector Protein under Abiotic Stress

Molecular Cloning and Functional Characterization of the Dehydrin (IpDHN) Gene From Ipomoea pes-caprae

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