Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum

Hamachi, Ashita; Nisihara, Masahiro; Saito, Shiori; Rim, Hojun; Takahashi, Hitomi; Islam, Monirul; Uemura, Takuya; Ohnishi, Toshiyuki; Ozawa, Rika; Maffei, Massimo E.; Arimura, Gen Ichiro

doi:10.1007/s00425-018-3054-z

Cited by 9 publications

(8 citation statements)

References 61 publications

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“…Some plants, such as Rhazya stricta L., have shown an adaptation to the hottest daylight hours based on increased PIP aquaporins expression in leaves [ 67 ], similar to the strawberry ( Fragraria vesca L.), which increases PIP expression after one-hour of heat stress [ 68 ]. On the contrary, in tobacco plants, there is a decrease in the PIP2s levels in roots after 50 °C treatment [ 69 ]. Moreover, the pattern of aquaporin gene expression can be different in response to heat stress among cultivars of the same plant species, as described in Setaria italic L. [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

Dual Inoculation with Rhizophagus irregularis and Bacillus megaterium Improves Maize Tolerance to Combined Drought and High Temperature Stress by Enhancing Root Hydraulics, Photosynthesis and Hormonal Responses

Romero-Munar

Aroca

Zamarreño

et al. 2023

IJMS

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Climate change is leading to combined drought and high temperature stress in many areas, drastically reducing crop production, especially for high-water-consuming crops such as maize. This study aimed to determine how the co-inoculation of an arbuscular mycorrhizal (AM) fungus (Rhizophagus irregularis) and the PGPR Bacillus megaterium (Bm) alters the radial water movement and physiology in maize plants in order to cope with combined drought and high temperature stress. Thus, maize plants were kept uninoculated or inoculated with R. irregularis (AM), with B. megaterium (Bm) or with both microorganisms (AM + Bm) and subjected or not to combined drought and high temperature stress (D + T). We measured plant physiological responses, root hydraulic parameters, aquaporin gene expression and protein abundances and sap hormonal content. The results showed that dual AM + Bm inoculation was more effective against combined D + T stress than single inoculation. This was related to a synergistic enhancement of efficiency of the phytosystem II, stomatal conductance and photosynthetic activity. Moreover, dually inoculated plants maintained higher root hydraulic conductivity, which was related to regulation of the aquaporins ZmPIP1;3, ZmTIP1.1, ZmPIP2;2 and GintAQPF1 and levels of plant sap hormones. This study demonstrates the usefulness of combining beneficial soil microorganisms to improve crop productivity under the current climate-change scenario.

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Section: Discussionmentioning

confidence: 99%

Dual Inoculation with Rhizophagus irregularis and Bacillus megaterium Improves Maize Tolerance to Combined Drought and High Temperature Stress by Enhancing Root Hydraulics, Photosynthesis and Hormonal Responses

Romero-Munar

Aroca

Zamarreño

et al. 2023

IJMS

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“…2) targeting gentian Gt5GT , Gt3 ′ GT , and Gt5/3 ′ AT genes were constructed for a subsequent plant transformation. A basic vector, pSbar-pcoCas9, was first constructed by replacing the uidA ( gus ) gene of pSKAN221-HT 26 with the plant codon-optimized SpCas9 (pcoCas9) 27 . Two single-guide RNA (sgRNA) expression cassettes targeting the gentian Gt5GT , Gt3 ′ GT , and Gt5/3 ′ AT genes were introduced into pSbar-pcoCas9 in tandem via a restriction enzyme digestion and ligation.…”

Section: Methodsmentioning

confidence: 99%

Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers

Tasaki

Higuchi

Watanabe

et al. 2019

Sci Rep

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Genome editing by the CRISPR/Cas9 system has recently been used to produce gene knockout lines in many plant species. We applied this system to analyze Japanese gentian plants that produce blue flowers because of the accumulation of a polyacylated anthocyanin, gentiodelphin. Mutant lines in which anthocyanin modification genes were knocked out were examined to assess the contribution of each gene to the blue pigmentation of flowers. The targeted genes encoded anthocyanin 5-O-glycosyltransferase (Gt5GT), anthocyanin 3′-O-glycosyltransferase (Gt3′GT), and anthocyanin 5/3′-aromatic acyltransferase (Gt5/3′AT). The Gt5GT knockout lines accumulated delphinidin 3G, whereas the Gt3′GT knockout lines accumulated delphinidin 3G-5CafG as the major flower pigment. Knocking out Gt5/3′AT resulted in the accumulation of delphinidin 3G-5G-3′G and delphinidin 3G-5G as the primary and secondary pigments, respectively. These results indicated the existence of two pathways mediating the modification of delphinidin 3G-5G in flowers, with one involving a glycosylation by 3′GT and the other involving an acylation by 5/3′AT. The Gt5GT, Gt3′GT, and Gt5/3′AT transformants produced pale red violet, dull pink, and pale mauve flowers, respectively, unlike the vivid blue flowers of wild-type plants. Thus, the glycosylation and subsequent acylation of the 3′-hydroxy group of the B-ring in delphinidin aglycone is essential for the development of blue gentian flowers.

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“…The GES gene, which was first cloned and functionally identified in sweet basil ( O. basilicum ), was shown to have the function of geraniol catalysis [ 6 ]. Subsequently, GES has been fairly extensively studied in many plant species, such as C. sinensis [ 12 ], L. odoratus [ 13 ], Gardenia jasminoides [ 30 ], N. tabacum [ 31 ], and P. bellina [ 15 ]. The second pathway for the production of geraniol is a phosphatase-based non-canonical pathway for monoterpene biosynthesis that involves NUDX [ 9 , 11 ].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, DoGES1 was ectopically expressed in N. benthamiana leaves, resulting in significantly increased geraniol content in transgenic leaves, compared to the control group ( Figure 6 ). Although some TPS proteins have been shown to catalyze the formation of multiple products [ 25 , 30 , 31 ], GES is highly specific and produces only geraniol, similar to linalool synthase, which makes a single acyclic monoterpene alcohol, linalool [ 6 , 7 , 19 , 20 ]. Therefore, DoGES1 mainly acts as a geraniol synthase in the synthesis of floral volatiles.…”

Section: Discussionmentioning

confidence: 99%

Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

Zhao

Silva³

et al. 2020

IJMS

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Floral scent is a key ornamental trait that determines the quality and commercial value of orchids. Geraniol, an important volatile monoterpene in orchids that attracts pollinators, is also involved in responses to stresses but the geraniol synthase (GES) responsible for its synthesis in the medicinal orchid Dendrobium officinale has not yet been identified. In this study, three potential geraniol synthases were mined from the D. officinale genome. DoGES1, which was localized in chloroplasts, was characterized as a geraniol synthase. DoGES1 was highly expressed in flowers, especially in petals. DoGES1 transcript levels were high in the budding stage of D. officinale flowers at 11:00 a.m. DoGES1 catalyzed geraniol in vitro, and transient expression of DoGES1 in Nicotiana benthamiana leaves resulted in the accumulation of geraniol in vivo. These findings on DoGES1 advance our understanding of geraniol biosynthesis in orchids, and lay the basis for genetic modification of floral scent in D. officinale or in other ornamental orchids.

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Overexpression of geraniol synthase induces heat stress susceptibility in Nicotiana tabacum

Cited by 9 publications

References 61 publications

Dual Inoculation with Rhizophagus irregularis and Bacillus megaterium Improves Maize Tolerance to Combined Drought and High Temperature Stress by Enhancing Root Hydraulics, Photosynthesis and Hormonal Responses

Dual Inoculation with Rhizophagus irregularis and Bacillus megaterium Improves Maize Tolerance to Combined Drought and High Temperature Stress by Enhancing Root Hydraulics, Photosynthesis and Hormonal Responses

Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers

Functional Characterization of a Dendrobium officinale Geraniol Synthase DoGES1 Involved in Floral Scent Formation

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