Overexpression of germin-like protein GmGLP10 enhances resistance to Sclerotinia sclerotiorum in transgenic tobacco

Zhang, Yuhang; Wang, Xuesong; Chang, Xingchao; Sun, Mingyang; Zhang, Yanzheng; Li, Wenbin; Li, Yongguang

doi:10.1016/j.bbrc.2018.02.046

Cited by 35 publications

(23 citation statements)

References 29 publications

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“…All significantly up-or downregulated DEGs had some connection with stress resistance or oxidation resistance in plants (Xiao et al 2018, Zhou et al 2010. OS08G0189900 encodes the germin-like protein GmGLP10 and enhanced resistance capacity to bacteria (Zhang et al 2017, Zhang et al 2018. OS01G0566500 can relieve oxidative stress in plants and is related to the MDA and SOD produced under cold-stress conditions (Saito et al 2010, Shen et al 2018; OS08G0509100 is related to the synthesis of lipoxygenase and drives the antioxidant ability of soybean (Glycine max) (Soccio et al 2018); OS01G0323600 allows the accumulation of S-adenosylmethionine synthetase transcripts under salt stress (Espartero et al 1994); OS07G0592600 can regulate cold or drought resistance via hormones such as abscisic acid (ABA) (Du et al 2012); and OS12G0147800 is a cold-induced gene in loquat (Eriobotrya japonica) (Song et al 2017).…”

Section: Transcriptome Analysis Of the Cold-tolerant Varietymentioning

confidence: 99%

Comprehensive Evaluation and Analysis of the Mechanism of Cold Tolerance Based on the Transcriptome of Weedy Rice Seedlings

Han

Cui

et al. 2020

Rice

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In this study, the cold-tolerance capacity of 133 varieties of weedy rice was evaluated based on the comprehensive evaluation index D, with Kongyu 131 used as a cold-tolerant control. A total of 39.8% of the 133 varieties were considered 'strong', indicating that weedy rice populations indeed have relatively strong coldtolerance capacity as a whole, and the robust cold-tolerant varieties WR29 and WR157 were identified. Regression analysis showed that the metrics including the nitrogen recovery index, superoxide dismutase (SOD) content and malondialdehyde (MDA) content correlated significantly (P < 0.05) with cold tolerance and could be used as indicators of cold tolerance. On the basis of a transcriptome analysis of WR157, a robust cold-tolerant variety identified in this study, a total of 4645 putative DEGs were identified in treated groups compared to the control groups, with 2123 upregulated DEGs and 2522 downregulated DEGs. All upregulated DEGs were enriched on 1388 terms, all downregulated DEGs were enriched on 1566 terms; 911 of the 2123 upregulated DEGs fell into 98 KEGG categories and 1103 of the 2522 downregulated DEGs were in 115 categories. Further analysis showed that GO: 0019740 and GO:0006808 are involved in nitrogen utilization; GO:0009269 and GO:0009414 are related to the stress response; and GO:0016491 and GO:0016614 are related to oxidoreductase activity. Background: Weedy rice (Oryza) is a related pest species of cultivated rice (Oryza sativa L.) that has strong abiotic stress resistance; however, the comprehensive mechanism governing its cold tolerance is poorly understood. Conclusion: Our comprehensive evaluation based on five morphological indices and nine physiological indicators revealed outstanding levels of cold-tolerance capacity among weedy rice varieties from different regions and revealed some terms related to cold tolerance via transcriptome analysis. Our results underscored the reliable evaluation methods for additional cold tolerance studies and revealed several genes related to cold tolerance, which will help researchers breed cultivated rice varieties to increase their cold-tolerance capacity. These traits have the ability to increase seedling survival rate and growth, as well as future yields.

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Section: Transcriptome Analysis Of the Cold-tolerant Varietymentioning

confidence: 99%

Comprehensive Evaluation and Analysis of the Mechanism of Cold Tolerance Based on the Transcriptome of Weedy Rice Seedlings

Han

Cui

et al. 2020

Rice

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“…Some GLPs has been proven to be SODs and against oxidative stress by ROS detoxification 14,29,30 . For instance, researchers have found that both GmGLP10 and HaGLP1 are SODs, their transgenic plants exhibit enhanced tolerance to Sclerotinia sclerotiorum infection, by promoting H 2 O 2 production 31,32 ; CchGLP exhibit Mn-SOD activity, its transgenic expression in tobacco provided resistance to geminivirus infection, and silencing of CchGLP in pepper (Capsicum chinense Jacq.) increased susceptibility to geminivirus single and mixed infections 33,34 .…”

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confidence: 99%

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

Pei

Zhu

Jia

et al. 2020

Sci Rep

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Germin-like proteins (GLps) are a diverse and ubiquitous family of plant glycoproteins belonging to the cupin super family; they play considerable roles in plant responses against various abiotic and biotic stresses. Here, we provide evidence that GLP2 protein from cotton (Gossypium hirsutum) functions in plant defense responses against Verticillium dahliae, Fusarium oxysporum and oxidative stress. Purified recombinant GhGLP2 exhibits superoxide dismutase (SOD) activity and inhibits spore germination of pathogens. Virus-induced silencing of GhGLP2 in cotton results in increased susceptibility to pathogens, plants exhibited severe wilt on leaves, enhanced vascular browning and suppressed callose deposition. transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing GhGLP2 showed significant resistance to V. dahliae and F. oxysporum, with reduced mycelia growth, increased callose deposition and cell wall lignification at infection sites on leaves. The enhanced tolerance of GhGLP2-transgenic Arabidopsis to oxidative stress was investigated by methyl viologen and ammonium persulfate treatments, along with increased H 2 o 2 production. further, the expression of several defense-related genes (PDF1.2, LOX2, and VSP1) or oxidative stress-related genes (RbohD, RbohF) was triggered by GhGLP2. Thus, our results confirmed the involvement of GhGLP2 in plant defense response against Verticillium and Fusarium wilt pathogens and stress conditions. Cotton (Gossypium hirsutum L.) is an important fiber crop that is considered the backbone of the global fiber economy 1. Verticillium and Fusarium wilt are caused by Verticillium dahliae and Fusarium oxysporum, respectively, which are soil-borne pathogenic fungi that present major constraints to the production of cotton 2. Verticillium wilt is a notorious and devastating disease of cotton 3 , and it occurs before the squaring stage and peaks in the boll-setting stage, causing necrotic areas on the leaves, wilting, and discoloration of the vascular tissues 4. The disease symptoms of Fusarium wilt initiate and peak at the seedling and squaring stages, respectively, showing necrotic patches between the main veins and leaf detachment from the stem 5. Due to diverse factors, such as the climate, pathogen population structures, and cultivar susceptibility, the currently available control measures for these two diseases are not adequate 6. Thus, research on the cultivation of resistant cotton plants, by finding novel disease-resistant genes against these soil-borne fungal species, is consequently, of great importance. Previous studies have suggested that extracellular germins and germin-like proteins (GLPs) could be induced by a range of abiotic or biotic stresses, such as herbivores 7,8 , drought 9,10 , salinity 8,11 and they are considered to be the pathogenesis-related proteins (PRs) 16 family due to their disease resistance property 12-14. Germins and GLPs were first characterized in wheat (Triticum aestivum) and constitute a large plant gene family 15. They occur as water-solub...

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“…Previous studies have shown that several GLPs exhibit SOD activity, which leads to H 2 O 2 production and is often correlated with plant defense responses (Sultana et al, 2016; Zhang et al, 2017, 2018). In our study, the recombinant GhABP19 protein exhibited SOD activity in vitro .…”

Section: Resultsmentioning

confidence: 99%

GhABP19, a Novel Germin-Like Protein From Gossypium hirsutum, Plays an Important Role in the Regulation of Resistance to Verticillium and Fusarium Wilt Pathogens

Pei

Zhu

et al. 2019

Front. Plant Sci.

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Germin-like proteins (GLPs) are water-soluble plant glycoproteins belonging to the cupin superfamily. The important role of GLPs in plant responses against various abiotic and biotic stresses, especially pathogens, is well validated. However, little is known about cotton GLPs in relation to fungal pathogens. Here, a novel GLP gene was isolated from Gossypium hirsutum and designated as GhABP19 . The expression of GhABP19 was upregulated in cotton plants inoculated with Verticillium dahliae and Fusarium oxysporum and in response to treatment with jasmonic acid (JA) but was suppressed in response to salicylic acid treatment. A relatively small transient increase in GhABP19 was seen in H 2 O 2 treated samples. The three-dimensional structure prediction of the GhABP19 protein indicated that the protein has three histidine and one glutamate residues responsible for metal ion binding and superoxide dismutase (SOD) activity. Purified recombinant GhABP19 exhibits SOD activity and could inhibit growth of V. dahliae , F. oxysporum , Rhizoctonia solani , Botrytis cinerea , and Valsa mali in vitro . To further verify the role of GhABP19 in fungal resistance, GhABP19 -overexpressing Arabidopsis plants and GhABP19 -silenced cotton plants were developed. GhABP19-transgenic Arabidopsis lines showed much stronger resistance to V. dahliae and F. oxysporum infection than control (empty vector) plants did. On the contrary, silencing of GhABP19 in cotton conferred enhanced susceptibility to fungal pathogens, which resulted in necrosis and wilt on leaves and vascular discoloration in GhABP19 -silenced cotton plants. The H 2 O 2 content and endogenous SOD activity were affected by GhABP19 expression levels in Arabidopsis and cotton plants after inoculation with V. dahliae and F. oxysporum , respectively. Furthermore, GhABP19 overexpression or silencing resulted in activation or suppression of JA-mediated signaling, respectively. Thus, GhABP19 plays important roles in the regulation of resistance to verticillium and fusarium wilt in plants. These modulatory roles were exerted by its SOD activity and ability to activate the JA pathway. All results suggest that GhABP19 was involved in plant disease resistance.

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Overexpression of germin-like protein GmGLP10 enhances resistance to Sclerotinia sclerotiorum in transgenic tobacco

Cited by 35 publications

References 29 publications

Comprehensive Evaluation and Analysis of the Mechanism of Cold Tolerance Based on the Transcriptome of Weedy Rice Seedlings

Comprehensive Evaluation and Analysis of the Mechanism of Cold Tolerance Based on the Transcriptome of Weedy Rice Seedlings

Molecular evidence for the involvement of cotton GhGLP2, in enhanced resistance to Verticillium and Fusarium Wilts and oxidative stress

GhABP19, a Novel Germin-Like Protein From Gossypium hirsutum, Plays an Important Role in the Regulation of Resistance to Verticillium and Fusarium Wilt Pathogens

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