Overexpression of hsa-miR-30a-5p and nonobstructive azoospermia: A case-control study

Arefnia, Mohammad; Motovali-Bashi, Majid; Javadirad, Seyed-Morteza; Norioun, Hamid

doi:10.18502/ijrm.v20i5.11054

Cited by 4 publications

(4 citation statements)

References 22 publications

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“…Similarly, in olive flounder ( Paralichthys olivaceus ), miR-143, miR-26a and let-7a were also identified as the most expressed miRNAs in both ovaries and testes (Gu et al, 2014). In mouse and human, miR-30a-5p was crucial for spermatogonial stem cell differentiation (Khanehzad et al, 2021) and was used as a marker for men suffering from non-obstructive azoospermia (Arefnia et al, 2022). In our data, however, we observed a conserved overexpression of miR-30a in adult fish ovaries compared to adult testes in the ten studied species.…”

Section: Discussionmentioning

confidence: 99%

Identification of sex-biased miRNA markers informative of heat-past events

van Gelderen,

Montfort,

Álvarez-Dios

et al. 2024

Preprint

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Elevated temperatures during early developmental stages play a pivotal role in the fate of the final sexual phenotype of fish populations, particularly towards male-skewed sex ratios . This is the case with European sea bass (Dicentrarchus labrax), one of the most important European species in the aquaculture industry. To unveil informative markers of the past thermal events, we investigated changes in the miRNome within the gonads of this species. Consequently, we exposed European sea bass to elevated temperatures during early development and conducted miRNA-sequencing analysis in the ovaries and testes one year post-heat treatment. The examination of miRNA expression levels identified three and twelve miRNAs in ovaries and testes, respectively, reflecting past thermal events. To assess the evolutionary conservation of these identified miRNAs in gonads, we cross-referenced our data with miRNome public information from ovaries and testes in nine additional fish species. This analysis uncovered 43 potential sex-biased markers present in at least five studied species along the evolutionary timeline. For instance, miR-155, miR-429, and miR-140 were female-skewed while miR-184, miR-499, and miR-135 were male-skewed. In addition, among these markers, eight conserved sex-skewed miRNAs proved informative regarding past thermal events in both the ovaries (e.g., miR-192-5p and miR-143-3p) and testes (miR-129-5p, miR-2187a-3p, miR-724-5p, miR-143-3p, miR-194a-5p and miR-223-3p). Notably, miR-223-3p and miR-194a-5p were conserved female-skewed, but showed upregulation in males exposed to high temperature. These miRNAs could serve as markers of heat-induced masculinization of genetic female-prone fish. The current research broadens the inventory of sex-specific miRNAs along evolution in fish and, elucidates thermosensitive miRNAs in the gonads. These findings hold promise as potential tools for predicting historical environmental events associated with masculinization due to high-temperature treatments in cultured species but also perhaps for natural populations exposed to a climate change scenario.

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Section: Discussionmentioning

confidence: 99%

Identification of sex-biased miRNA markers informative of heat-past events

van Gelderen,

Montfort,

Álvarez-Dios

et al. 2024

Preprint

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“…As a result, the downstream genes TNP1, PRM1, and PRM2 remain silent. All of these factors combine to induce male infertility in men with NOA ( 39 ). MiR-126 can stimulate cell proliferation and restrain the apoptosis of immature porcine Sertoli cells by targeting the PIK3R2 gene.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive analysis of microRNA and metabolic profiles in bovine seminal plasma of different semen quality

et al. 2023

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BackgroundSeminal plasma plays a pivotal role in modulating sperm viability and function. However, the underlying mechanisms have not been fully elucidated.MethodIn this study, the bull semen production records of core breeding farms and bull stations in the past 10 years were analyzed.ResultsWe found that the semen of 5-year-old bulls collected for the first time is of the best quality (p < 0.05). Despite the bull semen collected under the above conditions, low-quality sperm is still obtained from part of bulls due to individual differences. Interestingly, seminal plasma from normal semen is capable of improving low-quality semen motility. To identify the potential key factors in seminal plasma, the differences in miRNA and metabolite profiles between normal and low-quality seminal plasma were analyzed. We found that 59 miRNAs were differently expressed, including 38 up-regulated and 21 down-regulated miRNAs. Three hundred and ninety-one and 327 significantly different metabolites were identified from the positive and negative ion models, respectively. These multiple miRNAs and metabolites collectively contribute to the motility of sperm, subsequently, affect semen quality.DiscussionTogether, these results not only revealed the critical factors of seminal plasma improving sperm quality but also provided potential miRNA- or metabolite-based biomarkers to identify the high semen quality.

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“…In addition, it was discovered that mir-122a can reduce TNP2 expression during spermatogenesis. Arefnia et al [ 27 ] demonstrated that the purpose of this miRNA is to inhibit gene expression by degrading the transcription of this gene.…”

Section: Discussionmentioning

confidence: 99%

Polymorphisms and expression levels of TNP2, SYCP3, and AZFa genes in patients with azoospermia

Jebur,

Dastmalchi,

Banamolaei

et al. 2023

Clin Exp Reprod Med

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Objective: Azoospermia (the total absence of sperm in the ejaculate) affects approximately 10% of infertile males. Despite diagnostic advances, azoospermia remains the most challenging issue associated with infertility treatment. Our study evaluated transition nuclear protein 2 (TNP2) and synaptonemal complex protein 3 (SYCP3) polymorphisms, azoospermia factor a (AZFa) microdeletion, and gene expression levels in 100 patients with azoospermia. Methods: We investigated a TNP2 single-nucleotide polymorphism through polymerase chain reaction (PCR) restriction fragment length polymorphism analysis using a particular endonuclease. An allele-specific PCR assay for SYCP3 was performed utilizing two forward primers and a common reverse primer in two PCR reactions. Based on the European Academy of Andrology guidelines, AZFa microdeletions were evaluated by multiplex PCR. TNP2, SYCP3, and the AZFa region main gene (DEAD-box helicase 3 and Y-linked [DDX3Y]) expression levels were assessed via quantitative PCR, and receiver operating characteristic curve analysis was used to determine the diagnostic capability of these genes. Results: The TNP2 genotyping and allelic frequency in infertile males did not differ significantly from fertile volunteers. In participants with azoospermia, the allelic frequency of the SYCP3 mutant allele (C allele) was significantly altered. Deletion of sY84 and sY86 was discovered in patients with azoospermia and oligozoospermia. Moreover, SYCP3 and DDX3Y showed decreased expression levels in the azoospermia group, and they exhibited potential as biomarkers for diagnosing azoospermia (area under the curve, 0.722 and 0.720, respectively). Conclusion: These results suggest that reduced SYCP3 and DDX3Y mRNA expression profiles in testicular tissue are associated with a higher likelihood of retrieving spermatozoa in individuals with azoospermia. The homozygous genotype TT of the SYCP3 polymorphism was significantly associated with azoospermia.

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Overexpression of hsa-miR-30a-5p and nonobstructive azoospermia: A case-control study

Cited by 4 publications

References 22 publications

Identification of sex-biased miRNA markers informative of heat-past events

Identification of sex-biased miRNA markers informative of heat-past events

Comprehensive analysis of microRNA and metabolic profiles in bovine seminal plasma of different semen quality

Polymorphisms and expression levels of TNP2, SYCP3, and AZFa genes in patients with azoospermia

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