Overexpression of <i>Hevea brasiliensis</i> ethylene response factor <i>Hb<scp>ERF</scp>‐<scp>IX</scp>c5</i> enhances growth and tolerance to abiotic stress and affects laticifer differentiation

Lestari, Retno; Rio, Maryannick; Martin, Florence; Leclercq, Julie; Woraathasin, Natthakorn; Roques, Sandrine; Dessailly, Florence; Clément‐Vidal, Anne; Sanier, Christine; Fabre, Denis; Melliti, Semi; Suharsono, Suharsono; Montoro, Pascal

doi:10.1111/pbi.12774

Cited by 45 publications

(25 citation statements)

References 60 publications

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“…Although these two transcription factors are orthologues to ERF1, which controls a large panel of defence genes, there is no evidence for the activation of genes encoding ROS-scavenging enzymes ( Piyatrakul et al 2014 ). Interestingly, overexpression of these two HbERF genes conferred a better tolerance to abiotic stress ( Lestari et al Submitted ).…”

Section: Towards a Comprehensive Analysis Of Redox-related Genes In mentioning

confidence: 99%

Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

2016

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Environmental stress can lead to oxidative stress resulting from an increase in reactive oxygen species (ROS) and involves redox adjustments. Natural rubber is synthesized in laticifers, which is a non-photosynthetic tissue particularly prone to oxidative stress. This paper reviews the current state of knowledge on the ROS production and ROS-scavenging systems in laticifers. These regulations have been the subject of intense research into a physiological syndrome, called Tapping Panel Dryness (TPD), affecting latex production in Hevea brasiliensis. In order to prevent TPD occurrence, monitoring thiol content appeared to be a crucial factor of latex diagnosis. Thiols, ascorbate and γ-tocotrienol are the major antioxidants in latex. They are involved in membrane protection from ROS and likely have an effect on the quality of raw rubber. Some transcription factors might play a role in the redox regulatory network in Hevea, in particular ethylene response factors, which have been the most intensively studied given the role of ethylene on rubber production. Current challenges for rubber research and development with regard to redox systems will involve improving antioxidant capacity using natural genetic variability.

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Section: Towards a Comprehensive Analysis Of Redox-related Genes In mentioning

confidence: 99%

Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

2016

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“…Although a large number of genomic data and transcriptome data of rubber tree can be obtained [9][10][11], but few functional genes have been proved in rubber tree [12][13][14][15]. Functional gene analysis is still a major challenge lacking e cient and reliable genetic transformation system [15,16].…”

Section: Discussionmentioning

confidence: 99%

“…The physiological functions of rubber molecule and latex remain intrigue [7,8]. To date, rubber biosynthesis pathway has been well characterized [8], the whole-genome data and transcriptome database became available in rubber tree [9][10][11], but only a few NR biosynthesis-related genes functions are evaluated by transgenic rubber tree due to low genetic transformation e ciency [12][13][14][15]. The development of an e cient and reliable rubber tree transformation system is still challenging [15,16].…”

Section: Introductionmentioning

confidence: 99%

Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Wang

Zhu

et al. 2020

Preprint

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BackgroundSince it is very difficult to obtain gene knockouts in rubber tree (Hevea Brasiliensis) due to low genetic transformation efficiency. Virus-induced gene silencing (VIGS) is a powerful gene silencing tool that has been intensively applied in plant. Up to now, the application of VIGS in rubber tree has not yet been reported.ResultsHevea brasiliensis phytoene desaturase (HbPDS) was identified in H. brasiliensis genome. The prediction of small interfering RNAs (siRNAs) from HbPDS and the silencing gene fragment (SGF) were predicted and a length of 409 bp SGF was chosen to be tested. We show that the tobacco rattle virus (TRV) -VIGS is able to induce effective HbPDS silencing in rubber tree. The TRV-VIGS system has the potential for functional gene studies in rubber tree.ConclusionsThis is the first time to report VIGS in rubber tree. The present TRV-VIGS method could be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree. The applied TRV-VIGS method will achieve deeper underground into the natural rubber biosynthesis and regulation in this important rubber-producing plant.

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“…Moreover, in the present study we observed an increase in the ERF1 expression that may be connected with the oxidative stress present in the NC. ERF1 is upregulated during the stress response to abiotic stressors such as water deficit, cold and salt treatment (Lestari et al 2018;Makhloufi et al 2014). The ethylene and jasmonate pathways converge in the transcriptional activation of ERF1 in order to prevent the progression of a disease in Arabidopsis (Lorenzo et al 2003).…”

Section: Discussionmentioning

confidence: 99%

Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

Betekhtin

Piński

Milewska‐Hendel

et al. 2019

Plant Cell Tiss Organ Cult

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The morphogenic callus (MC) of Fagopyrum tataricum contains a large amount of flavonoids, especially rutin, and exhibits a high level of antioxidant activity. A non-morphogenic callus (NC) may appear on the surface of the MC after two to three years of cultivation and is then subjected to a consistently high level of oxidative stress. The elucidation of the molecular background of this instability is essential for gaining a better understanding of the somaclonal variation mechanisms in tissue cultures that have different morphogenic potentials. Thus, in this study we show that continuous oxidative stress in a NC might be connected with a rapid senescence process and as a result, in the upregulation of the genes that are connected with the telomere complexity, ethylene biosynthesis and the expression of DNA methyltransferases. Moreover, we analysed the presence of the hydroxyproline-rich glycoproteins in the calli and demonstrated the differences between the MC and NC. The LM2 antibody can be useful as a marker of the cells in the MC that are embryogenically determined, while the MAC207 antibody seems to be a positive marker of a MC as its signal was absent in the NC. This study also provides the first report on the effect of trichostatin A on the DNA methyltransferases and demethylases in a MC. Key message LM2 can be used as a marker of the embryogenically determined cells. Overproduction of ethylene is present in the NC.

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Overexpression of Hevea brasiliensis ethylene response factor HbERF‐IXc5 enhances growth and tolerance to abiotic stress and affects laticifer differentiation

Cited by 45 publications

References 60 publications

Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

Reactive oxygen species in Hevea brasiliensis latex and relevance to Tapping Panel Dryness

Tobacco rattle virus -induced gene silencing in Hevea brasiliensis

Stability and instability processes in the calli of Fagopyrum tataricum that have different morphogenic potentials

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