Overexpression of LpCPC from Lilium pumilum confers saline-alkali stress (NaHCO₃) resistance

Abstract

Lilium Pumilum with wide distribution is highly tolerant to salinity. The blue copper protein LpCPC ( Lilium pumilum Cucumber Peeling Cupredoxin ) gene was cloned from Lilium pumilum , which has the conserved regions of type I copper protein. Moreover, LpCPC has the closest relation to CPC from Actinidia chinensis using DNAMAN software and MEGA7 software. qRT-PCR indicated that LpCPC expression was higher in root and bulb of Lilium pumilum , and the expression of the LpCPC gene increased and reached the highest level at 12 h in bulbs under 20 mM NaHCO 3 . The transgenic yeast was more tolerant compared with the control under NaHCO 3 stress. Compared with the wild type, overexpressing plants indicated a relatively lower degree of wilting. In addition, the chlorophyll content, soluble phenol content, and lignin content of overexpressing lines were higher than that of wild-type, whereas the relative conductivity of overexpressing plants was significantly lower than that of wild-type plants. Expression of essential genes including NHX1 and SOS1 in salt stress response pathways are steadily higher in overexpression tobacco than that in wild-types. Transgenic lines had much higher levels of CCR1 and CAD , which are involved in lignin production, compared with wild-type lines. The yeast two-hybrid technique was applied to screen probable interacting proteins interacting with LpCPC. Eight proteins interacted with LpCPC were screened, and five of which were demonstrated to be associated with plant salinity resistance. Overall, the role of gene LpCPC is mediating molecule responses in increasing saline-alkali stress resistance, indicating that it is an essential gene to enhance salt tolerance in Lilium pumilum .

Genome-Wide Identification of the Phytocyanin Gene Family and Its Potential Function in Salt Stress in Soybean (Glycine max (L.) Merr.)

“…Previous research has demonstrated the pivotal role of PCs in plant adaptation to salt stress [41,42]. To explore whether GmPCs participate in the response mechanism of salt stress, based on the reported transcriptome sequencing data from roots and leaves of soybean cultivar Jack seedlings treated with 150 mM NaCl solution for 6 h [37], the co-expression network of GmPC gene family members was analyzed by WGCNA.…”