Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Zhou, Rui; Fan, Menglong; Zhao, Mei; Jiang, Xinqiang; Liu, Qinghua

doi:10.1080/15592324.2022.2031783

Cited by 12 publications

(7 citation statements)

References 63 publications

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“…KNOX possesses homologous heterotypic structural domains, and it belongs to the three-amino-acid-loop-extension ( TALE ) family, which plays important roles in the maintenance of cellular totipotency and the regulation of leaf development [ 35 ]. KNOX family transcription factors underlie lobed leaf morphogenesis in multiple plants such as Lilium tsingtauense [ 36 ], citrus [ 37 ], barley [ 38 ], and Arabidopsis [ 35 , 39 , 40 ]. In lettuce, LsKN1 not only binds the promoter of LsPID to up-regulate the synthesis of growth hormone but also binds specifically to the LsAS1 promoter to down-regulate LsAS1 expression and synergistically regulate the formation of palmately lobed leaves [ 41 , 42 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Chromosomal Regions and Candidate Genes for Round leaf Locus in Cucumis melo L.

Fang,

Zhu,

et al. 2024

Plants

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Leaf morphology plays a crucial role in plant classification and provides a significant model for studying plant diversity while directly impacting photosynthetic efficiency. In the case of melons, leaf shape not only influences production and classification but also represents a key genetic trait that requires further exploration. In this study, we utilized forward genetics to pinpoint a recessive locus, dubbed Cmrl (Round leaf), which is responsible for regulating melon leaf shape. Through bulked segregant analysis sequencing and extensive evaluation of a two-year F2 population, we successfully mapped the Cmrl locus to a 537.07 kb region on chromosome 8 of the melon genome. Subsequent genetic fine-mapping efforts, leveraging a larger F2 population encompassing 1322 plants and incorporating F2:3 phenotypic data, further refined the locus to an 80.27 kb interval housing five candidate genes. Promoter analysis and coding sequence cloning confirmed that one of these candidates, MELO3C019152.2 (Cmppr encoding a pentatricopeptide repeat-containing family protein, Cmppr), stands out as a strong candidate gene for the Cmrl locus. Notably, comparisons of Cmrl expressions across various stages of leaf development and different leaf regions suggest a pivotal role of Cmrl in the morphogenesis of melon leaves.

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Section: Discussionmentioning

confidence: 99%

Identification of Chromosomal Regions and Candidate Genes for Round leaf Locus in Cucumis melo L.

Fang,

Zhu,

et al. 2024

Plants

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“…Based on the HMM model of the KNOX structural domain, 49 potential KNOX genes were identi ed in Moso bamboo genomic database. However, after rigorous screening, including the exclusion of genes with E-value ≤ 15 , only 24 KNOX family genes were identi ed. These genes were renamed PeKNOX01-PeKNOX24 based on their positions on the chromosomal scaffold (Table S1).…”

Section: Identi Cation and Characterization Of Knox Genesmentioning

confidence: 99%

“…KNOX genes have been studied in various plants, including gymnosperms like Pinus sylvestris, monocots such as rice and orchids, and dicots like Arabidopsis, poplars and pears [15]. In Arabidopsis, the Class I subfamily is expressed only at the SAM and comprises STM, KNAT1, KNAT2, and KNAT6.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of the KNOX gene family in Moso bamboo: Insights into their role in promoting the rapid shoot growth

Jiao,

Tan,

Guo

et al. 2023

Preprint

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Background KNOTTED1-like homeobox (KNOX) genes, plant-specific homologous box transcription factors (TFs), play a central role in regulating plant growth, development, organ formation, and response to biotic and abiotic stresses. However, a comprehensive genome-wide identification of the KNOX genes in Moso bamboo (Phyllostachys edulis), the fastest growing plant, has not yet been conducted, and the specific biological functions of this family remain unknown. Results The expression profiles of 24 KNOX genes, divided into two subfamilies, were determined by integrating Moso bamboo genome and its transcriptional data. The KNOX gene promoters were found to contain several light and stress-related cis-acting elements. Synteny analysis revealed stronger similarity with rice KNOX genes than with Arabidopsis KNOX genes. Additionally, several conserved structural domains and motifs were identified in the KNOX proteins. The expansion of the KNOX gene family was primarily regulated by tandem duplications. Furthermore, the KNOX genes were responsive to NAA and GA hormones, exhibiting distinct temporal expression patterns in four different organs of Moso bamboo. Short Time-series Expression Miner (STEM) analysis and quantitative PCR (qPCR) assays demonstrated that PeKNOX genes may play a role in promoting rapid shoot growth. Additionally, Gene Ontology (GO) and Protein-Protein Interaction (PPI) network enrichment analyses revealed several functional annotations for PeKNOXs. By regulating downstream target genes, PeKNOXs are involved in the synthesis of AUX /IAA, ultimately affecting cell division and elongation. Conclusions In the present study, we identified and characterized a total of 24 KNOX genes in Moso bamboo and investigated their physiological properties and conserved structural domains. To understand their functional roles, we conducted an analysis of gene expression profiles using STEM and RNA-seq data. This analysis successfully revealed regulatory networks involving both upstream and downstream genes of KNOX. Furthermore, KNOX genes are involved in the AUX/IAA metabolic pathway, which accelerates shoot growth by influencing downstream target genes. These results provide a theoretical foundation for studying the molecular mechanisms underlying the rapid growth and establish the groundwork for future research into the functions and transcriptional regulatory networks of the KNOX gene family.

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“…To explore the molecular mechanism of BlOFP3 and BlOFP5 in leaf development, six genes, including KNAT2, KNAT6, AS1, CLV1, GA20ox1, PIN1 and CUC2 genes, have been proved as crucial genes in leaf development (Long et al, 1996;Belles-Boix et al, 2006;Wang et al, 2021;Zhou et al, 2022), were chosen for expression analysis in the transgenic A. thaliana plants. As shown in figure 8, the expressions of KNAT2 and KNAT6 were both drastically inhibited in plants overexpressing BlOFP3 and BlOFP5 (Figure 8).…”

Section: Expression Analysis Of Genes Associated With Leaf Developmen...mentioning

confidence: 99%

Genome-wide identification and characterization of OVATE family proteins in Betula luminifera reveals involvement of BlOFP3 and BlOFP5 genes in leaf development

Borah

Ying

et al. 2022

Front. Plant Sci.

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Ovate family proteins (OFP) are plant-specific transcription factors involved in regulating morphologies of the lateral organs, plant growth and development. However, the functional roles of OFP genes in Betula luminifera, an important timber tree species, are not well studied. In this study, we identified 20 BlOFP genes and analyzed their phylogenetic relationship, gene structure, conserved motifs, and cis-elements. Further, expression analysis indicates that BlOFP genes were up-regulated in leaves on the one-year-old branch compared to leaves on the current-year branch and bract, except BlOFP7, BlOFP11, BlOFP14 and BlOFP12. The overexpression of BlOFP3 and BlOFP5 in Arabidopsis thaliana not only resulted in a slower growth rate but also produced sawtooth shape, flatter and darker green rosette leaves. Further investigation showed that the leaf thickness of the transgenic plants was more than double that of the wild type, which was caused by the increasement in the number and size of palisade tissue cells. Furthermore, the expression analysis also indicated that the expressions of several genes related to leaf development were significantly changed in the transgene plants. These results suggested the significant roles of BlOFP3 and BlOFP5 in leaf development. Moreover, protein-protein interaction studies showed that BlOFP3 interacts with BlKNAT5, and BlOFP5 interacts with BlKNAT5, BlBLH6 and BlBLH7. In conclusion, our study demonstrates that BlOFP3 and BlOFP5 were involved in leaf shape and thickness regulation by forming a complex with BlKNAT5, BlBLH6 and BlBLH7. In addition, our study serves as a guide for future functional genomic studies of OFP genes of the B. luminifera.

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Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Cited by 12 publications

References 63 publications

Identification of Chromosomal Regions and Candidate Genes for Round leaf Locus in Cucumis melo L.

Identification of Chromosomal Regions and Candidate Genes for Round leaf Locus in Cucumis melo L.

Genome-wide analysis of the KNOX gene family in Moso bamboo: Insights into their role in promoting the rapid shoot growth

Genome-wide identification and characterization of OVATE family proteins in Betula luminifera reveals involvement of BlOFP3 and BlOFP5 genes in leaf development

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