Overexpression of integrin α11 induces cardiac fibrosis in mice

Abstract

We report for the first time that overexpression of integrin α11 induces cardiac fibrosis and left ventricular hypertrophy. This is a result of changes in intracellular hypertrophic signalling and secretion of soluble factors that increase collagen production in the heart.

Characterization of adhesion properties of the cardiomyocyte integrins and extracellular matrix proteins using atomic force microscopy

“…The reduction effect was dramatically enhanced when CMs were pretreated with these two antibodies at the same time. These data indicated that CMs interact with CN through α 10 ‐ and α 11 ‐integrin, consistent with previous studies that α 10 ‐ and α 11 ‐integrin are collagen‐binding receptors on cells . Mean force and binding probability reduced significantly when CMs pretreated with anti‐α 3 ‐, ‐α 5 ‐integrin antibodies or both interacted with FN.…”

“…The CN, FN, and LN are three ECM proteins binding to different integrins, corresponding to different physiological functions . The data in the present study showed that the unbinding force of LN‐CM integrins has the greatest value, whereas FN‐CM integrins have the smallest under the same loading rate (Figure 2).…”

“…For example, during the formation of murine induced pluripotent stem cell‐derived embryoid bodies, CN and β 1 ‐integrin were found to be expressed and upregulated synergistically around day 3 in differentiation, indicating that CN‐integrins interactions are needed for cardiogenesis . The expression of CN‐binding integrins was found to be associated with cardiac fibrosis and left ventricular remodeling . It was reported that FN and FN‐binding integrins were required for cardiac morphogenesis and associated with cardiac hypertrophy .…”

“…11 The expression of CN-binding integrins was found to be associated with cardiac fibrosis and left ventricular remodeling. 12,13 It was reported that FN and FN-binding integrins were required for cardiac morphogenesis and associated with cardiac hypertrophy. [14][15][16] Data from Okada et al suggested that LN-binding integrins may protect the myocardium from ischemic injury by modulating important integrinrelated signaling pathways.…”

Google maps for tissues: Multiscale imaging of biological systems and disease

“…1 Another group of MR techniques for tissue characterization makes use of the relaxation time T 2 to probe for tissue water changes or the apparent water diffusion coefficient (ADC) to monitor changes in the intra-and extracellular volume. The capabilities of parametric MRI using the MR relaxation parameter T 1 for non-invasive tissue characterization were employed to demonstrate that overexpression of integrin α11 induces cardiac fibrosis and left ventricular hypertrophy in mice.…”