Overexpression of KIF2A is Suppressed by miR-206 and Associated with Poor Prognosis in Ovarian Cancer

Abstract

Background/Aims: This study aimed to investigate the expression and prognostic value of kinesin family member 2A (KIF2A) and the suppression effects of microRNA-206 (miR-206) on KIF2A in ovarian cancer. Methods: Ovarian cancer tissues from patients and ovarian cancer cell lines (A2780 and SKOV3) were used in this study. miR-206 mimics and control were transiently transfected into cells. RT-qPCR was performed to detect KIF2A mRNA and miR-206 expression levels, Western blot was performed to detect KIF2A protein levels, Dual-Luciferase Reporter Assay was used to examine the inhibition effects of miR-206 on KIF2A mRNA, immunohistochemical staining was used to examine the expression of KIF2A in tissue sections. CCK-8, transwell and Annexin-V-FITC/Propidium Iodide staining with flow cytometry were used to detect the cell proliferation, migration/invasion, and apoptosis respectively. Results: Our study explored the expression profiles of KIF2A and miR-206 in the patients with ovarian cancer. We found that overexpression of KIF2A was associated with a poor prognosis in ovarian cancer. We also found that KIF2A mRNA contains two target sites for miR-206 binding and confirmed that miR-206 directly suppresses KIF2A; inhibits ovarian cancer cell proliferation, migration, and invasion; and induces apoptosis. Conclusion: The results suggest KIF2A could serve a valuable prognostic indicator in ovarian cancer and provide a rationale for treatment of ovarian cancer by targeting KIF2A via miR-206.

Weighted Gene Coexpression Network Analysis of Features That Control Cancer Stem Cells Reveals Prognostic Biomarkers in Lung Adenocarcinoma

“…The KIF gene family, which encodes proteins, is involved in many important physiological processes, especially intracellular transport, chromosome separation, mitotic spindle formation and cytokinesis. Some studies have suggested that mutations in the KIF gene family are involved in the formation of many cancers (Sheng et al, 2018;Xia et al, 2018;Xie et al, 2018). There are few studies on KIF18b and LUAD, and only confirmed that LUAD patients with high KIF18b expression have a poor prognosis.…”

KIF18B promotes the proliferation of pancreatic ductal adenocarcinoma via activating the expression of CDCA8

“…KIF14 promotes tumor progression and is associated with poor prognosis in gastric cancer (Yang et al, ). KIF2A is suppressed by a micro RNA, miR‐206 and correlated with the poor prognosis in ovarian cancer (Sheng et al, ). These finds, together with our results in this study, indicated the key function of kinesin family in cancer development.…”

Kinesin family member 2A high expression correlates with advanced tumor stages and worse prognosis in non‐small cell lung cancer patients

“…The kinesin proteins are microtubules depolymerases, which are involved in mitosis, cell migration, trafficking, and cell signaling . KIF2A, as one member of the kinesin protein family, has attracted a lot of attention due to its implication in the development and progression of several tumors . For example, in gastric cancer, KIF2A is upregulated in cancerous tissues compared with adjacent non‐tumor tissues and its high expression is correlated with advanced TNM stage and LYN metastasis .…”

“…The predictive value of KIF2A in prognosis is reported in several tumors . One study exhibits a correlation between KIF2A expression and the 5‐year survival rate in patients with gastric cancer .…”

“…Previous evidence exhibits that KIF2A serves as a minus‐end depolymerizing motor of the microtubule, which is essential for invading neighboring tissues as well as the lymph node invasion and distant metastasis . Furthermore, the carcinogenic effect of KIF2A in development and progression of several cancers, including gastric, breast, and ovarian cancer, has been observed . However, for the role of KIF2A in NSCLC, limited information is available.…”