Overexpression of long intergenic noncoding RNA LINC00312 inhibits the invasion and migration of thyroid cancer cells by down-regulating microRNA-197-3p

Abstract

The study evaluated the ability of long intergenic noncoding RNA LINC00312 (LINC00312) to influence the proliferation, invasion, and migration of thyroid cancer (TC) cells by regulating miRNA-197-3p. TC tissues and adjacent normal tissues were collected from 211 TC patients. K1 (papillary TC), SW579 (squamous TC), and 8505C (anaplastic TC) cell lines were assigned into a blank, negative control (NC), LINC00312 overexpression, miR-197-3p inhibitors, and LINC00312 overexpression + miR-197-3p mimics group. The expression of LINC00312, miR-197-3p, and p120 were measured using quantitative real-time PCR (qRT-PCR) and Western blotting. Cell proliferation was assessed via CCK8 assay, cell invasion through the scratch test, and cell migration via Transwell assay. In comparison with adjacent normal tissues, the expression of LINC00312 is down-regulated and the expression of miR-197-3p is up-regulated in TC tissues. The dual luciferase reporter gene assay confirmed that P120 is a target of miR-197-3p. The expression of LINC00312 and p120 was higher in the LINC00312 overexpression group than in the blank and NV groups. However, the expression of miR-197-3p was lower in the LINC00312 overexpression group than in the blank and NC groups. The miR-197-3p inhibitors group had a higher expression of miR-197-3p, but a lower expression of p120 than the blank and NC groups. The LINC00312 overexpression and miR-197-3p inhibitor groups had reduced cell proliferation, invasion and migration than the blank and NC groups. These results indicate that a LINC00312 overexpression inhibits the proliferation, invasion, and migration of TC cells and that this can be achieved by down-regulating miR-197-3p.

LINC00312 represses proliferation and metastasis of colorectal cancer cells by regulation of miR‐21

“…Cui et al have reported that lncRNA CCAT1 promotes glioma tumourigenesis by sponging miR‐181b, thereby leading to derepression of its endogenous targets FGFR3 and PDGFRα; these findings point to a potential therapeutic target in glioma . A recent study verified that LINC00312 is associated with miR‐197‐3p and modulates the expression of a miR‐197‐3p target, p120, thereby providing powerful evidence that LINC00312 enhances gene expression at the post‐transcriptional level . Here, we revealed a potential binding site within LINC00312 and miR‐21 by bioinformatic prediction.…”

“…23,24 A recent study verified that LINC00312 is associated with miR-197-3p and modulates the expression of a miR-197-3p target, p120, thereby providing powerful evidence that LINC00312 enhances gene expression at the post-transcriptional level. 20 Here, we revealed a potential binding site within LINC00312 and miR-21 by bioinformatic prediction. Given that miR-21 is overexpressed in CRC [25][26][27] and promotes cell growth and invasion by repressing PTEN in CRC, 15,17 we hypothesized that LINC00312 might function as a miR-21 sponge to enhance PTEN expression.…”

“…Zhang et al found that LINC00312 inhibits proliferation and invasiveness of nasopharyngeal carcinoma cells . Wang et al and Liu et al revealed that overexpression of LINC00312 inhibits the invasion and migration of bladder and thyroid cancer cells . Zhu et al reported that LINC00312 inhibits cell proliferation and promotes apoptosis via HOXA5 in non‐small cell lung cancer .…”

LINC00312/YBX1 Axis Regulates Myofibroblast Activities in Oral Submucous Fibrosis

“…LINC00312 has been demonstrated to be a negative regulator of the estrogen receptor α, and activates the JNK2/AP-1/MMP1 pathway to enhance the invasion of NPC [12]. In other types of cancers, overexpression of LINC00312 was able to inhibit the migration and invasion of bladder and thyroid cancer cells by targeting miR-197-3p [13,14]. LINC00312 also has been shown to diminish the aggressiveness of thyroid cancer through suppression of the PI3K/Akt pathway [15].…”

FOXD2‐AS1 correlates with the malignant status and regulates cell proliferation, migration, and invasion in cutaneous melanoma

“…8,9 In the past five years, more and more lncRNAs have been demonstrated to play an important role in carcinogenesis. 8,9 In the past five years, more and more lncRNAs have been demonstrated to play an important role in carcinogenesis.…”