Overexpression of MAGT1 is associated with aggressiveness and poor prognosis of colorectal cancer

Zheng, Kaiyu; Yang, Qianqiong; Xie, Lang; Qiu, Zhenghua; Huang, Yongsheng; Lin, Yongwei; Tu, Lingjing; Cui, Chunhui

doi:10.3892/ol.2019.10710

Cited by 15 publications

(20 citation statements)

References 25 publications

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“…Subsequently, we found that miR-628-5p could bind to the 3ʹUTR of MAGT1, which was closely associated with human cancer progression. 16,17 We next measured the level of MAGT1 and found was highly expressed in glioma tissues and cells, whereas its level was conspicuously downregulated in glioma cells treated with Sev. Besides, circ_0002755 modulated MAGT1 expression via interacting with miR-628-5p.…”

Section: Discussionmentioning

confidence: 99%

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<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Xia

Guan³

et al. 2020

CMAR

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Background: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration. Methods: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo. Results: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo. Conclusion: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis.

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Section: Discussionmentioning

confidence: 99%

“…Zheng et al reported that overexpression of MAGT1 led to the poor prognosis of colorectal cancer. 16 Wang et al found that microRNA-199a-5p inhibited glioma progression by inhibiting MAGT1. 17 Therefore, MAGT1 might be an attracting drug target for glioma and its function in Sev-mediated glioma progression should be explored.…”

Section: Introductionmentioning

confidence: 99%

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Xia

Guan³

et al. 2020

CMAR

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“…TRPM7 expression was also associated with tumor infiltration, tumor grade, and the presence of distant metastasis [ 81 , 82 ]. In qRT-PCR-based and in silico studies, the Mg 2+ transporter MAGT1 was found to be overexpressed in colorectal cancerous tissues [ 83 ]. High MAGT1 expression also correlates with chemotherapeutic resistance, metastatic status, and tumor stage [ 83 ].…”

Section: Expression Of Mg 2+ Transporters In DImentioning

confidence: 99%

“…In qRT-PCR-based and in silico studies, the Mg 2+ transporter MAGT1 was found to be overexpressed in colorectal cancerous tissues [ 83 ]. High MAGT1 expression also correlates with chemotherapeutic resistance, metastatic status, and tumor stage [ 83 ]. CNNM4 protein was also found downregulated in an IHC analysis of cancerous colorectal tissues, and inversely correlates with tumor malignancy [ 84 ].…”

Section: Expression Of Mg 2+ Transporters In DImentioning

confidence: 99%

Mg2+ Transporters in Digestive Cancers

et al. 2021

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Despite magnesium (Mg2+) representing the second most abundant cation in the cell, its role in cellular physiology and pathology is far from being elucidated. Mg2+ homeostasis is regulated by Mg2+ transporters including Mitochondrial RNA Splicing Protein 2 (MRS2), Transient Receptor Potential Cation Channel Subfamily M, Member 6/7 (TRPM6/7), Magnesium Transporter 1 (MAGT1), Solute Carrier Family 41 Member 1 (SCL41A1), and Cyclin and CBS Domain Divalent Metal Cation Transport Mediator (CNNM) proteins. Recent data show that Mg2+ transporters may regulate several cancer cell hallmarks. In this review, we describe the expression of Mg2+ transporters in digestive cancers, the most common and deadliest malignancies worldwide. Moreover, Mg2+ transporters’ expression, correlation and impact on patient overall and disease-free survival is analyzed using Genotype Tissue Expression (GTEx) and The Cancer Genome Atlas (TCGA) datasets. Finally, we discuss the role of these Mg2+ transporters in the regulation of cancer cell fates and oncogenic signaling pathways.

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“…And the evolutionary rate of MAGT1 (magnesium transporter 1) was 0.33127 in extremely large carnivores, 6.1 times of that in background group. Loss of MAGT1 would disrupt T cell signaling and lead to a novel human primary immunode ciency [55], and furthermore, overexpression of MAGT1 was associated with development and metastasis of colorectal cancer [56]. Here, we obtained 100 cancer-related genes that were signi cantly associated with body size evolution in carnivores including 15 cancer-related REGs that identi ed in extremely large group, which might protect them from cancer invasion, special for large and long-lived species.…”

Section: Molecular Evidence For the Peto's Paradox In Carnivores?mentioning

confidence: 99%

New insights into the genetic mechanisms of body size evolution in Carnivora

Huang¹,

Sun²,

Wu³

et al. 2020

Preprint

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Background The range of body sizes in Carnivora is unparalleled in any other mammalian order, with more than 130,000 times in body mass and 50 times in length. However, the molecular mechanisms underlying the huge difference in body size of Carnivora have not been explored so far. Results Herein, we performed a comparative genomics analysis of 20 carnivores to explore the genetic basis of great body size variation in carnivores. Phylogenetic generalized least squares (PGLS) revealed that 337 genes were significantly related to both head body length and body mass, these genes were defined as body size associated genes (BSAGs). Fourteen positively related BSAGs were found to be associated with obesity and three of which were identified to be under rapid evolution in the extremely large body-sized carnivores, which suggested that these obesity-related BSAGs might have driven the body size expansion in carnivores. Interestingly, 100 BSAGs were examined to be associated with cancer control in carnivores, particularly 15 cancer-related genes were found to be under rapid evolution in extremely large carnivores. These results strongly suggested that large body-sized carnivores might have evolved effective mechanism to resist cancer, which could be regarded as molecular evidence to support for the Peto’s paradox. For small carnivores, we identified 15 rapidly evolving genes and found six genes with fixed amino acid changes that were reported to reduce body size. Conclusion This study brings new insights into the molecular mechanisms that drove the diversifying evolution of body size in carnivores, and provides new target genes for exploring the mysteries of body size evolution in mammals.

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Overexpression of MAGT1 is associated with aggressiveness and poor prognosis of colorectal cancer

Cited by 15 publications

References 25 publications

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

<p>Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis</p>

Mg2+ Transporters in Digestive Cancers

New insights into the genetic mechanisms of body size evolution in Carnivora

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