Overexpression of maize GOLDEN2 in rice and maize calli improves regeneration by activating chloroplast development

Luo, Wenjun; Tan, Jiantao; Li, Tie; Feng, Ziting; Ding, Zhi; Xie, Xianrong; Chen, Yuanling; Chen, Letian; Liu, Yao-Guang; Zhu, Qinlong; Guo, Jun

doi:10.1007/s11427-022-2149-2

Cited by 9 publications

(3 citation statements)

References 40 publications

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“…The transformation efficiency is proportional to the regeneration efficiency. The overexpression of maize GOLDEN2, a GARP transcription factor superfamily member that regulates several biological processes and phytohormone signaling pathways in plants, enhanced the regeneration of rice and maize calli by activating chloroplast development ( Luo et al, 2023 ). Similarly, the homologs of GOLDEN2 from legumes ( Wang et al, 2013 ) could help enhance regeneration in legumes as well.…”

Section: Strategies To Enhance Transformationmentioning

confidence: 99%

Recalcitrance to transformation, a hindrance for genome editing of legumes

Nivya,

Shah

2023

Front. Genome Ed.

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Plant genome editing, a recently discovered method for targeted mutagenesis, has emerged as a promising tool for crop improvement and gene function research. Many genome-edited plants, such as rice, wheat, and tomato, have emerged over the last decade. As the preliminary steps in the procedure for genome editing involve genetic transformation, amenability to genome editing depends on the efficiency of genetic engineering. Hence, there are numerous reports on the aforementioned crops because they are transformed with relative ease. Legume crops are rich in protein and, thus, are a favored source of plant proteins for the human diet in most countries. However, legume cultivation often succumbs to various biotic/abiotic threats, thereby leading to high yield loss. Furthermore, certain legumes like peanuts possess allergens, and these need to be eliminated as these deprive many people from gaining the benefits of such crops. Further genetic variations are limited in certain legumes. Genome editing has the potential to offer solutions to not only combat biotic/abiotic stress but also generate desirable knock-outs and genetic variants. However, excluding soybean, alfalfa, and Lotus japonicus, reports obtained on genome editing of other legume crops are less. This is because, excluding the aforementioned three legume crops, the transformation efficiency of most legumes is found to be very low. Obtaining a higher number of genome-edited events is desirable as it offers the option to genotypically/phenotypically select the best candidate, without the baggage of off-target mutations. Eliminating the barriers to genetic engineering would directly help in increasing genome-editing rates. Thus, this review aims to compare various legumes for their transformation, editing, and regeneration efficiencies and discusses various solutions available for increasing transformation and genome-editing rates in legumes.

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Section: Strategies To Enhance Transformationmentioning

confidence: 99%

Recalcitrance to transformation, a hindrance for genome editing of legumes

Nivya,

Shah

2023

Front. Genome Ed.

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“…Moreover, the overexpression of maize GLKs in calli was shown to improve the ability of rice and maize to regenerate (Luo et al, 2022). A recent study found that OsGLK1 also participates in tapetum plastid development and programmed cell death, consequently affecting pollen fertility in rice (Zheng et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

“…In accordance with this, the ectopic expression of maize GLK s in rice can boost biomass and grain yield by facilitating chloroplast development and photosynthesis (Li et al, 2020; Yeh et al, 2022). Moreover, the overexpression of maize GLK s in calli was shown to improve the ability of rice and maize to regenerate (Luo et al, 2022). A recent study found that OsGLK1 also participates in tapetum plastid development and programmed cell death, consequently affecting pollen fertility in rice (Zheng et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

OsNF-YB7 inactivates OsGLK1 to inhibit chlorophyll biosynthesis in rice embryo

Yang,

Bai,

et al. 2024

Preprint

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As a master regulator of seed development, Leafy Cotyledon 1 (LEC1) promotes chlorophyll (Chl) biosynthesis inArabidopsis, but the mechanism underlying this remains poorly understood. Here, we found that loss of function ofOsNF-YB7, aLEC1homolog of rice, leads to chlorophyllous embryo, indicating thatOsNF-YB7plays an opposite role in Chl biosynthesis in rice compared with that inArabidopsis. OsNF-YB7 regulates the expression of a group of genes responsible for Chl biosynthesis and photosynthesis by directly binding to their promoters. In addition, OsNF-YB7 interacts with Golden 2-Like 1 (OsGLK1) to inhibit the transactivation activity of OsGLK1, a key regulator of Chl biosynthesis. Moreover, OsNF-YB7 can directly repressOsGLK1expression by recognizing its promoterin vivo, indicating the involvement of OsNF-YB7 in multiple regulatory layers of Chl biosynthesis in rice embryo. We propose that OsNF-YB7 functions as a transcriptional repressor to regulate Chl biosynthesis in rice embryo.

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