Overexpression of METTL3 associated with the metabolic status on ¹⁸F-FDG PET/CT in patients with Esophageal Carcinoma

Abstract

Background: To investigate the expression of methyltransferase 3 (METTL3) and its relationship with 18 F-FDG uptake in patients with esophageal carcinoma (ESCA). Materials and methods:This study analyzed the expression of METTL3 in ESCA and its relationship with clinicopathological features by The Cancer Genome Atlas (TCGA) database. Immunohistochemical staining was performed on 57 tumor tissues of ESCA patients who underwent PET/CT scan before surgery to evaluate the expression of METTL3, glucose transporter 1 (GLUT1), and hexokinase 2 (HK2) in tumor tissues and peritumoral tissues. Analyze the relationship between SUVmax with METTL3, HK2, and GLUT1 expression. Results: The expression of METTL3, GLUT1, and HK2 was significantly increased in ESCA tissues compared with normal tissues (p < 0.001). The expression of METTL3 was correlated with tumor size and histological differentiation (p < 0.05), and there was no significant difference between age, sex, pathological types, tumor staging, or lymph node metastasis (p > 0.05). The SUVmax was significantly higher in tumors with high METTL3 expression (17.822±6.249) compared to low METTL3 expression (9.573±5.082) (p < 0.001). There was a positive correlation between the SUVmax and METTL3 expression in ESCA (r 2 = 0.647, p < 0.001). Multivariate analysis confirmed the association between SUVmax and METTL3 expression (p < 0.05). GLUT1 and HK2 expression in ESCA was positively correlated with 18 F-FDG uptake and METTL3 status (p < 0.001). Conclusions: The high expression of METTL3 is related to the high SUVmax in ESCA, and METTL3 may increase 18 F-FDG uptake by regulating GLUT1 and HK2.

NPM1 Is a Prognostic Biomarker Involved in Immune Infiltration of Lung Adenocarcinoma and Associated With m6A Modification and Glycolysis

“…Finally, we verified the differential expression of NPM1 in LUAD and normal samples by qRT-PCR and IHC staining. The specific procedures refer to previous studies (14), and see the Supplementary Materials for details.…”

RETRACTED: MTTL3 upregulates microRNA-1246 to promote occurrence and progression of NSCLC via targeting paternally expressed gene 3

“…Consistently, it has been recently reported that METTL3 expression was related to TNM stage in patients with nasopharyngeal carcinoma, 8 as well as tumor size in patients with esophageal carcinoma. 17 Liu et al. 18 have clarified that high METTL3 expression was associated with poor prognosis of patients with oral squamous cell carcinoma.…”

Comprehensive Analysis of GLUT1 Immune Infiltrates and ceRNA Network in Human Esophageal Carcinoma

“…Currently, studies have shown that GLUT1 is highly expressed in oral (4), gastric (5), breast (6), colorectal (7), and ovarian cancers (8). In previous studies (9), we found that GLUT1 was highly expressed in ESCA and strongly correlated with metabolic parameters of 18 F-FDG PET/CT imaging, but we failed to carry out more studies on the biological function of GLUT1 in ESCA.…”

“…Clinical samples were obtained from 50 patients with ESCA who were surgically treated at Taihe Hospital Affiliated of Hubei University of Medicine from February 2016 to September 2017. The content of GLUT1 was detected by IHC according to the method previously described (9). The ESCA tissue and the paracarcinoma tissues were prepared into 3 mm paraffin sections and incubated with mouse monoclonal antibodies of GLUT1 (1:200, Abcam, USA) at 4°C overnight in a refrigerator.…”