Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway

Yang, Haitao; Zhang, Jun; Li, Jiuping; Zhao, Fang; Yao, Shukun; Xing, Xuemei

doi:10.3892/etm.2018.6700

Cited by 12 publications

(16 citation statements)

References 28 publications

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“…Abnormal expression of miRNA has been found in various human cancer types. The genes encoding miR-574-3p are located on chromosomes 4P (4p14 and 4p15) with a tumor inhibitory miRNA function [13]. MiR-574-3p expression levels were decreased in spinal chordoma patients [14].…”

Section: Discussionmentioning

confidence: 99%

MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer

Gao

et al. 2019

Bioscience Reports

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Colorectal cancer (CRC) remains the candidate for one of the typical types of malignant tumors of in gastrointestinal tract all around the world, which leads to tremendous death and ranks as the top leading death of cancer. Recently, microRNAs have emerged as double-edged sword in numerous cancers. This investigation aims to discuss the regulative role of microRNA-574-3p (miR-574-3p), elucidating its molecular mechanism and clinical significance in CRC. Herein, it revealed to us that miR-574-3p was lowly expressed in CRC tissues in comparison with the matched paracarcinoma tissues. In addition, transfection of SW480 and HT29 cells with miR-574-3p mimics prohibited the post-transcriptional expression of Cyclin D2 (CCND2), which then significantly blocked cell growth and cell migration, yet triggered cell apoptosis. Also, dual-luciferase reporter assays proved the role of CCND2 as the targeted gene for miR-574-3p. miR-574-3p overexpression prohibited the activity of CCND2 in SW480 and HT29 cells. Silencing of CCND2 in SW480 and HT29 CRC cell lines leading to reduced cell proliferative and migrative rates, and enhanced apoptotic rate. The suppressive effects of elevation of miR-574-3p on the proliferation of the human CRC cells and promotive effects on cell apoptosis by targeting CCND2 were further illustrated in the in vitro studies. Thus, we hypothesize that miR-574-3p may be served as a prospective therapeutic candidate for CRC.

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Section: Discussionmentioning

confidence: 99%

MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer

Gao

et al. 2019

Bioscience Reports

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“…In other studies, miR-574 was decreased in liver recipients suffering AR, although these differences were not observed compared with biopsies with AMR [47]. Yang et al [54] showed that miR-574-3p negatively modulates the IL-6/STAT3 pathway. Thus, the decrease in miR-574-3p expression in samples with T cell-mediated rejection [47] could increase pro-inflammatory pathways derived from STAT3 activation [54].…”

Section: Discussionmentioning

confidence: 88%

“…Yang et al [54] showed that miR-574-3p negatively modulates the IL-6/STAT3 pathway. Thus, the decrease in miR-574-3p expression in samples with T cell-mediated rejection [47] could increase pro-inflammatory pathways derived from STAT3 activation [54].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA Expression Changes in Kidney Transplant: Diagnostic Efficacy of miR-150-5p as Potential Rejection Biomarker, Pilot Study

Alfaro

Legáz

Jiménez-Coll

et al. 2021

JCM

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Background: The kidney allograft biopsy is considered the gold standard for rejection diagnosis but is invasive and could be indeterminate. Several publications point to the role of miRNA expression in suggesting its involvement in the acceptance or rejection of organ transplantation. This study aimed to analyze microRNAs involved in the differentiation and activation of B and T lymphocytes from kidney transplant (KT) patients’ peripheral blood leukocytes to be used as biomarkers of acute renal rejection (AR). Methods: A total of 15 KT patients with and without acute rejection (AR/NAR) were analyzed and quantified by miRNA PCR array. A total of 84 miRNAs related to lymphocyte differentiation and activation B and T were studied. The functions and biological pathways were analyzed to predict the potential targets of differential expressed miRNAs. Results: Six miRNA were increased in the AR group (miR-191-5p, miR-223-3p, miR-346, miR-423-5p, miR-574-3p, and miR-181d) and miR-150-5p was increased in the NAR group. In silico studies showed a total of 2603 target genes for the increased miRNAs in AR, while for the decrease miRNA, a total of 1107 target-potential genes were found. Conclusions: Our results show that KT with AR shows a decrease in miR-150-5p expression compared to NAR, suggesting that the decrease in miR-150-5p could be related to an increased MBD6 whose deregulation could have clinical consequences.

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“…Further, miR-125a promotes the fundamental shift from inflammation to immune suppression by controlling regulatory T cells homeostasis (43). Some evidence suggests that miR-574-3p may also have antiinflammatory function (24). Overall, the presence of several target genes implicated in the regulation of the inflammatory response suggested that the highly expressed miR-125a-5p, miR-574-3p, and let-7-5p subset may play a role in dampening the pro-inflammatory activity of dILC3.…”

Section: Discussionmentioning

confidence: 99%

“…The miR-574-3p has been reported as an oncosuppressor miRNA in different neoplasms, inhibiting cell proliferation, migration and/or metastasis (23)(24)(25). Interestingly, miR-574-3p targets IL6/JAK/STAT3 pathway in hematopoietic cells (24) and TGF-β1 can up-regulate its expression (26). The miRNAs let-7e-5p and miR-125a-5p belong to the miRNA cluster miR-99b/let-7e/miR-125a, a genomic region embedded in the protein coding gene SPACA6 and localized in the q13.41 on chromosome 19.…”

Section: A Restricted Subset Of Mirnas Is Highly Expressed In Dilc3mentioning

confidence: 99%

An Anti-inflammatory microRNA Signature Distinguishes Group 3 Innate Lymphoid Cells From Natural Killer Cells in Human Decidua

et al. 2020

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Innate lymphoid cells (ILCs) are a heterogeneous subset of lymphocytes deeply implicated in the innate immune responses to different pathogens, in lymphoid organogenesis and in the maintenance of tissue homeostasis. Group 3 innate lymphoid cells (ILC3) have been detected in human decidua, where they play a role in the early inflammatory phase favoring implantation and tissue remodeling as well as in the subsequent regulatory phase preventing fetal rejection and supporting neoangiogenesis. A balance between inflammation and immune tolerance is required to maintain pregnancy, thus maternal immune system must be controlled by finely tuned mechanisms. microRNAs (miRNAs) are small non-coding RNAs with important regulatory roles in immune cells, but their function in decidual ILC3 (dILC3) and in decidual NK (dNK) cells is still undefined. Here, we examined the miRNome by microarray in these cells during the first trimester of pregnancy and compared with miRNA profiles of peripheral blood NK (pbNK) cells from pregnant women. We show that distinct miRNA profiles could clearly distinguish dILC3 from NK cells. Correlation analyses revealed that dNK and pbNK miRNome profiles are more similar to each other as compared to dILC3. In particular, we identified 302 and 279 mature miRNAs differentially expressed in dILC3 as compared to dNK and pbNK, respectively. The expression of miR-574-3p and the miR-99b/let-7e/miR-125a miRNA cluster resulted the most increased in dILC3. Remarkably, gene ontology analysis and pathway enrichments of miRNA targets revealed an involvement of these miRNAs in the promotion of anti-inflammatory responses. In agreement to this finding, we also found a higher expression of the anti-inflammatory miR-146a-5p in dILC3 with respect to NK cells. Overall, our data identified specific miRNA signatures distinguishing dILC3, dNK, and pbNK cells. Our data suggest the existence of a tight epigenetic control mediated by miRNAs in dILC3, potentially acting as a brake to prevent exaggerated inflammatory responses and to maintain the immune homeostasis in the early phases of pregnancy.

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Overexpression of miR‑574‑3p suppresses proliferation and induces apoptosis of chronic myeloid leukemia cells via targeting IL6/JAK/STAT3 pathway

Cited by 12 publications

References 28 publications

MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer

MiRNA-574-3p inhibits cell progression by directly targeting CCND2 in colorectal cancer

MicroRNA Expression Changes in Kidney Transplant: Diagnostic Efficacy of miR-150-5p as Potential Rejection Biomarker, Pilot Study

An Anti-inflammatory microRNA Signature Distinguishes Group 3 Innate Lymphoid Cells From Natural Killer Cells in Human Decidua

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