Overexpression of mRNA‑decapping enzyme 1a affects survival rate in colorectal carcinoma

Wu, Chen; Liu, Weizhen; Ruan, Tuo; Zhu, Xiaojie; Tao, Kaixiong; Zhang, Weikang

doi:10.3892/ol.2018.8730

Cited by 14 publications

(13 citation statements)

References 32 publications

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“…In addition, Tang, Y. 's study found that the high expression of DCP1A in colorectal carcinoma is correlated with poor prognosis [57], which is consistent with our results, indicating that the other PCGs and lncRNAs in our results could also be prognostic indicators for NFPA.…”

Section: Am J Biomed Sci and Ressupporting

confidence: 91%

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Epigenetic Role of Noncoding RNAs in the Recurrence of Pituitary Adenoma after Surgical Resection

Kim

2020

AJBSR

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Section: Am J Biomed Sci and Ressupporting

confidence: 91%

Section: Am J Biomed Sci and Resmentioning

confidence: 99%

Epigenetic Role of Noncoding RNAs in the Recurrence of Pituitary Adenoma after Surgical Resection

Kim

2020

AJBSR

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“… 21 , 22 Studies have shown that DCP1A expression is up-regulated in CRC, and its elevated expression is associated with the reduced survival rate in CRC patients. 23 , 24 Therefore, it may be a key target to regulate CRC progress.…”

Section: Introductionmentioning

confidence: 99%

<p>Circ_0007031 Serves as a Sponge of miR-760 to Regulate the Growth and Chemoradiotherapy Resistance of Colorectal Cancer via Regulating DCP1A</p>

Wang¹,

Wang²,

Zhang³

et al. 2020

CMAR

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Background: Colorectal cancer (CRC) is a kind of malignant tumor, and the development of chemoradiotherapy resistance (CRR) increases the difficulty of its treatment. The role of circular RNAs (circRNAs) in cancer progression has been well documented. Nevertheless, the function of circ_0007031 in the growth and CRR of CRC has not been well elucidated. Methods: CRR cell lines were constructed using 5-Fu and radiation. Cell counting kit 8 (CCK8) assay was employed to measure the 5-Fu resistance and proliferation of cells. Clonogenic assay was used to evaluate the radiation resistance of cells. Also, the expression of circ_0007031 and microRNA-760 (miR-760) was determined using quantitative real-time polymerase chain reaction (qRT-PCR). The cell cycle distribution and apoptosis of cells were assessed by flow cytometry. Besides, the levels of apoptosis-related protein and mRNAdecapping enzyme 1a (DCP1A) protein were measured by Western blot (WB) analysis. Further, dual-luciferase reporter assay and RNA immunoprecipitation (RIP) assay were used to confirm the interaction between miR-760 and circ_0007031 or DCP1A. In addition, animal experiments were performed to evaluate the function of silenced circ_0007031 on the 5-Fu and radiation resistance of CRC tumors. Results: Circ_0007031 expression was markedly increased in CRC tissues and cells, especially in CRC resistant cells. Circ_0007031 knockdown hindered proliferation, induced cell cycle arrest in the G0/G1 phase, enhanced apoptosis, and lowered the CRR of CRC resistant cells. Further, miR-760 could be targeted by circ_0007031, and its inhibitor could reverse the inhibition effect of circ_0007031 knockdown on the growth and CRR of CRC resistant cells. Moreover, DCP1A was a target of miR-760, and its overexpression could invert the suppression effect of miR-760 overexpression on the growth and CRR of CRC resistant cells. Circ_0007031 silencing could enhance the sensitivity of CRC tumors to 5-Fu and radiation to markedly reduce CRC tumor growth in vivo. Conclusion: Circ_0007031 might play a positive role in the CRR of CRC through regulating the miR-760/DCP1A axis, which might provide a new approach for treating the CRR of CRC.

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“…These studies indicated that MOV10 could be critical in tumorigenesis. DCP1A is a protein-coding gene for mRNA-decapping enzyme 1a, and several studies have revealed that DCP1A is upregulated in tumor tissues, such as malignant melanoma, colorectal carcinoma and gastric cancer (67)(68)(69). In addition, Tang et al (67) and Wu et al (68) reported that the high expression of DCP1A in colorectal carcinoma is correlated with poor prognosis, which is consistent with the current results, thus indicating that the other present PCGs and lncRNAs could also be prognostic indicators for NFPA.…”

Section: Discussionmentioning

confidence: 99%

Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence

et al. 2021

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Non-functioning pituitary adenoma (NFPA) is a very common type of intracranial tumor. Monitoring and predicting the postoperative recurrence of NFPAs is difficult, as these adenomas do not present with serum hormone hypersecretion. Long non-coding RNAs (lncRNAs) and protein-coding genes (PCGs) play critical roles in the development and progression of numerous tumors. However, the complex network of RNA interactions related to the mechanisms underlying the postoperative recurrence of NFPA is still unclear. In the present study, 73 patients with NFPA were investigated using high-throughput sequencing and follow-up investigations. In total, 6 of these patients with recurrence within 1 year after surgery were selected as the fast recurrence group, and 6 patients with recurrence 5 years after surgery were selected as the slow recurrence group. By performing differential expression analysis of the fast recurrence and slow recurrence groups, a set of differentially expressed PCGs and lncRNAs were obtained (t-test, P<0.05). Next, protein-protein interaction coregulatory networks and lncRNA-mRNA coexpression networks were identified. In addition, the hub lncRNA-mRNA modules related to NFPA recurrence were further screened and transcriptome expression markers for NFPA regression were identified (log-rank test, P<0.05). Finally, the ability of the hub and module genes to predict recurrence and progression-free survival in patients with NFPA was evaluated. To confirm the credibility of the bioinformatic analyses, nucleolar protein 6 and LL21NC02-21A1.1 were randomly selected from among the genes with prognostic significance for validation by reverse transcription-quantitative PCR in another set of NFPA samples (n=9). These results may be helpful for evaluating the slow and rapid recurrence of NFPA after surgery and exploring the mechanisms underlying NFPA recurrence. Future effective biomarkers and therapeutic targets may also be revealed.

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Overexpression of mRNA‑decapping enzyme 1a affects survival rate in colorectal carcinoma

Cited by 14 publications

References 32 publications

Epigenetic Role of Noncoding RNAs in the Recurrence of Pituitary Adenoma after Surgical Resection

Epigenetic Role of Noncoding RNAs in the Recurrence of Pituitary Adenoma after Surgical Resection

<p>Circ_0007031 Serves as a Sponge of miR-760 to Regulate the Growth and Chemoradiotherapy Resistance of Colorectal Cancer via Regulating DCP1A</p>

Identifying critical protein‑coding genes and long non‑coding RNAs in non‑functioning pituitary adenoma recurrence

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