Overexpression of MusaNAC68 reduces secondary wall thickness of xylem tissue in banana

Negi, Sanjana; Tak, Himanshu; Ganapathi, T. R.

doi:10.1007/s11816-019-00524-5

Cited by 18 publications

(8 citation statements)

References 39 publications

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“…Therefore, we speculate that the effect of metaxenia on lignin biosynthesis in pear fruit might be achieved by regulating hormone metabolism. In the early stage of fruit development, a large number of auxin signal transduction-related genes have significant differences in transcript abundance between YL and CL fruits, such as Aux/IAA (auxin/indoleacetic acids) family genes, auxin response factors (ARFs), auxin-responsive proteins, and indole-3-acetic acid-amido synthetase GH3s ( Supplementary Table S8) [47,48]. This phenomenon might have a large effect on the content of auxin and its signal transduction in pear fruit.…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have shown that auxin can inhibit the transcription of the lignin biosynthesis negative regulator BREVIPEDICELLUS (a member of the KNOX gene family) [35], thereby regulating the synthesis of lignin. In addition, Aux/IAAs and ARFs have also been shown to function to regulate lignin synthesis and SCW development [47]. Therefore, metaxenia might change the signal transduction process of auxin in pear fruit, thereby regulating the expression of TFs that are related to lignin metabolism and ultimately affecting lignin deposition and stone cell development (Figure 12).…”

Section: Resultsmentioning

confidence: 99%

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Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

Cheng

Zhang

Wang

et al. 2020

Forests

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The deposition of lignin in flesh parenchyma cells for pear stone cells, and excessive stone cells reduce the taste and quality of the fruit. The effect of metaxenia on the quality of fruit has been heavily studied, but the effect of metaxenia on stone cell formation has not been fully elucidated to date. This study used P. bretschneideri (Chinese white pear) cv. ‘Yali’ (high-stone cell content) and P. pyrifolia (Sand pear) cv. ‘Cuiguan’ (low-stone cell content) as pollination trees to pollinate P. bretschneideri cv. ‘Lianglizaosu’ separately to fill this gap in the literature. The results of quantitative determination, histochemical staining and electron microscopy indicated that the content of stone cells and lignin in YL fruit (‘Yali’ (pollen parent) × ‘Lianglizaosu’ (seed parent)) was significantly higher than that in CL fruit (‘Cuiguan’ (pollen parent) × ‘Lianglizaosu’ (seed parent)). The transcriptome sequencing results that were obtained from the three developmental stages of the two types of hybrid fruits indicated that a large number of differentially expressed genes (DEGs) related to auxin signal transduction (AUX/IAAs and ARFs), lignin biosynthesis, and lignin metabolism regulation (MYBs, LIMs, and KNOXs) between the CL and YL fruits at the early stage of fruit development. Therefore, metaxenia might change the signal transduction process of auxin in pear fruit, thereby regulating the expression of transcription factors (TFs) related to lignin metabolism, and ultimately affecting lignin deposition and stone cell development. In addition, we performed functional verification of a differentially expressed gene, PbC4H2 (cinnamate 4-hydroxylase). Heterologous expression of PbC4H2 in the c4h mutant not only restored its collapsed cell wall, but also significantly increased the lignin content in the inflorescence stem. The results of our research help to elucidate the metaxenia-mediated regulation of pear stone cell development and clarify the function of PbC4H2 in cell wall development and lignin synthesis, which establishes a foundation for subsequent molecular breeding.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

Cheng

Zhang

Wang

et al. 2020

Forests

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“…These transgenic banana plants showed stunted growth [ 42 , 43 ]. Moreover, the reduction of lignin was observed in transgenic bananas overexpressing MusaNAC68 and was considered to be linked with the increase in the height of transgenic bananas [ 44 ]. Although peroxidase increased in uniconazole-treated bananas at both 15 d and 25 d, p-coumaryl alcohol, the substrate of peroxidase, only increased at 25 d and decreased at 15 d. At 25 d, peroxidase expression was lower than that at 15 d. The inconsistency in the time and degree of change of the substrate p-coumaryl alcohol and peroxidase could not provide sufficient evidence for the change in lignin biosynthesis.…”

Section: Discussionmentioning

confidence: 99%

Integrated metabolomic and transcriptomic analyses of regulatory mechanisms associated with uniconazole-induced dwarfism in banana

LiuYan

Guo

et al. 2022

BMC Plant Biol

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Background Uniconazole is an effective plant growth regulator that can be used in banana cultivation to promote dwarfing and enhance lodging resistance. However, the mechanisms underlying banana dwarfing induced by uniconazole are unknown. In uniconazole-treated bananas, gibberellin (GA) was downregulated compared to the control groups. An integrative analysis of transcriptomes and metabolomes was performed on dwarf bananas induced by uniconazole and control groups. The key pathways involved in uniconazole-induced dwarfism in banana were determined according to the overlap of KEGG annotation of differentially expressed genes and (DEGs) differential abundant metabolites (DAMs). Results Compared with the control groups, the levels of some flavonoids, tannins, and alkaloids increased, and those of most lipids, amino acids and derivatives, organic acids, nucleotides and derivatives, and terpenoids decreased in uniconazole-treated bananas. Metabolome analysis revealed the significant changes of flavonoids in uniconazole-treated bananas compared to control samples at both 15 days and 25 days post treatment. Transcriptome analysis shows that the DEGs between the treatment and control groups were related to a series of metabolic pathways, including lignin biosynthesis, phenylpropanoid metabolism, and peroxidase activity. Comprehensive analysis of the key pathways of co-enrichment of DEGs and DAMs from 15 d to 25 d after uniconazole treatment shows that flavonoid biosynthesis was upregulated. Conclusions In addition to the decrease in GA, the increase in tannin procyanidin B1 may contribute to dwarfing of banana plants by inhibiting the activity of GA. The increased of flavonoid biosynthesis and the change of lignin biosynthesis may lead to dwarfing phenotype of banana plants. This study expands our understanding of the mechanisms underlying uniconazole-induced banana dwarfing.

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“…Currently, there has been one other NAC68 protein that was functionally characterized. MusaNAC68 conferred enhanced tolerance to drought and high salt stress when expressed in transgenic M. acuminata [ 64 ], and it reduced xylem secondary wall thickness [ 67 ]. Differently than MusaNAC68, CpNAC68 was downregulated by drought and confers tolerance to other stresses (i.e., cold, heat, osmotic, and salt) when ectopically expressed in Arabidopsis.…”

Section: Discussionmentioning

confidence: 99%

Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses

Lin

Liu

Wang

et al. 2021

Plants

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The NAC (NAM, ATAFs, CUC) family of transcription factors (TFs) play a pivotal role in regulating all processes of the growth and development of plants, as well as responses to biotic and abiotic stresses. Yet, the functions of NACs from non-model plant species remains largely uncharacterized. Here, we characterized the stress-responsive effects of a NAC gene isolated from wintersweet, an ornamental woody plant that blooms in winter when temperatures are low. CpNAC68 is clustered in the NAM subfamily. Subcellular localization and transcriptional activity assays demonstrated a nuclear protein that has transcription activator activities. qRT-PCR analyses revealed that CpNAC68 was ubiquitously expressed in old flowers and leaves. Additionally, the expression of CpNAC68 is induced by disparate abiotic stresses and hormone treatments, including drought, heat, cold, salinity, GA, JA, and SA. Ectopic overexpression of CpNAC68 in Arabidopsis thaliana enhanced the tolerance of transgenic plants to cold, heat, salinity, and osmotic stress, yet had no effect on growth and development. The survival rate and chlorophyll amounts following stress treatments were significantly higher than wild type Arabidopsis, and were accompanied by lower electrolyte leakage and malondialdehyde (MDA) amounts. In conclusion, our study demonstrates that CpNAC68 can be used as a tool to enhance plant tolerance to multiple stresses, suggesting a role in abiotic stress tolerance in wintersweet.

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Overexpression of MusaNAC68 reduces secondary wall thickness of xylem tissue in banana

Cited by 18 publications

References 39 publications

Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

Effects of Metaxenia on Stone Cell Formation in Pear (Pyrus bretschneideri) Based on Transcriptomic Analysis and Functional Characterization of the Lignin-Related Gene PbC4H2

Integrated metabolomic and transcriptomic analyses of regulatory mechanisms associated with uniconazole-induced dwarfism in banana

Transgene CpNAC68 from Wintersweet (Chimonanthus praecox) Improves Arabidopsis Survival of Multiple Abiotic Stresses

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