Overexpression of Myrothamnus flabellifolia MfWRKY41 confers drought and salinity tolerance by enhancing root system and antioxidation ability in Arabidopsis

Huang, Zhuo; Song, Li; Xiao, Yao; Zhong, Xiaojuan; Wang, Jiatong; Xu, Wenxin; Jiang, Cai‐Zhong

doi:10.3389/fpls.2022.967352

Cited by 9 publications

(3 citation statements)

References 51 publications

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“…Furthermore, PtoWAK106 was found to increase the stability of the PtoE2Fa‐PtoDPb1 complex (Figure 6e–j ). We also identified the up‐regulation of several drought‐resistance‐related genes, such as WRKY40 , NAC72 , and ERF5 , in PtoDPb1 ‐OE plants (Huang et al ., 2022 ; Li et al ., 2022 ; Yao et al ., 2021 ). It is therefore plausible to hypothesize that the expression of PtoWAK106 is induced by drought, which then modulates the function of PtoDPb1 and its downstream signalling pathways.…”

Section: Discussionmentioning

confidence: 99%

Allelic variations of WAK106‐E2Fa‐DPb1‐UGT74E2 module regulate fibre properties in Populus tomentosa

Wang,

Quan,

Qin

et al. 2023

Plant Biotechnology Journal

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SummaryWood formation, intricately linked to the carbohydrate metabolism pathway, underpins the capacity of trees to produce renewable resources and offer vital ecosystem services. Despite their importance, the genetic regulatory mechanisms governing wood fibre properties in woody plants remain enigmatic. In this study, we identified a pivotal module comprising 158 high‐priority core genes implicated in wood formation, drawing upon tissue‐specific gene expression profiles from 22 Populus samples. Initially, we conducted a module‐based association study in a natural population of 435 Populus tomentosa, pinpointing PtoDPb1 as the key gene contributing to wood formation through the carbohydrate metabolic pathway. Overexpressing PtoDPb1 led to a 52.91% surge in cellulose content, a reduction of 14.34% in fibre length, and an increment of 38.21% in fibre width in transgenic poplar. Moreover, by integrating co‐expression patterns, RNA‐sequencing analysis, and expression quantitative trait nucleotide (eQTN) mapping, we identified a PtoDPb1‐mediated genetic module of PtoWAK106‐PtoDPb1‐PtoE2Fa‐PtoUGT74E2 responsible for fibre properties in Populus. Additionally, we discovered the two PtoDPb1 haplotypes that influenced protein interaction efficiency between PtoE2Fa‐PtoDPb1 and PtoDPb1‐PtoWAK106, respectively. The transcriptional activation activity of the PtoE2Fa‐PtoDPb1 haplotype‐1 complex on the promoter of PtoUGT74E2 surpassed that of the PtoE2Fa‐PtoDPb1 haplotype‐2 complex. Taken together, our findings provide novel insights into the regulatory mechanisms of fibre properties in Populus, orchestrated by PtoDPb1, and offer a practical module for expediting genetic breeding in woody plants via molecular design.

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Section: Discussionmentioning

confidence: 99%

Allelic variations of WAK106‐E2Fa‐DPb1‐UGT74E2 module regulate fibre properties in Populus tomentosa

Wang,

Quan,

Qin

et al. 2023

Plant Biotechnology Journal

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“…The leaves were floated on a stomatal opening solution (10 mM KCl, 0.2 mM CaCl 2 , and 10 mM MES-KOH; pH, 6.15) for 2.5 h to induce stomatal opening. Then, the lower epidermis of leaves was placed in an opening solution with 7.5% PEG6000 and treated in a growth incubator for 2.5 h [ 60 ]. The stomata were observed and photographed using a microscope, and the width and length of 50 stomata per line were measured.…”

Section: Methodsmentioning

confidence: 99%

TaWRKY31, a novel WRKY transcription factor in wheat, participates in regulation of plant drought stress tolerance

Ge,

Tang,

Guan

et al. 2024

BMC Plant Biol

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Background Wheat, a crucial food crop in China, is highly vulnerable to drought stress throughout its growth and development. WRKY transcription factors (TFs), being one of the largest families of TFs, play a vital role in responding to various abiotic stresses in plants. Results Here, we cloned and characterized the TF TaWRKY31 isolated from wheat. This TF, belonging to the WRKY II family, contains a WRKYGQK amino acid sequence and a C2H2-type zinc finger structure. TaWRKY31 exhibits tissue-specific expression and demonstrates responsiveness to abiotic stresses in wheat. TaWRKY31 protein is localized in the nucleus and can function as a TF with transcription activating activity at the N-terminus. Results showed that the wheat plants with silenced strains (BSMV:TaWRKY31-1as and BSMV:TaWRKY31-2as) exhibited poor growth status and low relative water content when subjected to drought treatment. Moreover, the levels of O2·−, H2O2, and malondialdehyde (MDA) in the BSMV:TaWRKY31-induced wheat plants increased, while the activities of antioxidant enzymes (superoxide dismutase, peroxidase, and catalase) decreased. Compared to control plants, BSMV:TaWRKY31-induced wheat plants exhibited lower expression levels of TaSOD (Fe), TaPOD, TaCAT, TaDREB1, TaP5CS, TaNCED1, TaSnRK2, TaPP2C, and TaPYL5.Under stress or drought treatment conditions, the overexpression of TaWRKY31 in Arabidopsis resulted in decreased levels of H2O2 and MDA, as well as reduced stomatal opening and water loss. Furthermore, an increase in resistance oxidase activity, germination rate, and root length in the TaWRKY31 transgenic Arabidopsis was observed. Lastly, overexpression of TaWRKY31 in Arabidopsis resulted in higher the expression levels of AtNCED3, AtABA2, AtSnRK2.2, AtABI1, AtABF3, AtP5CS1, AtSOD (Cu/Zn), AtPOD, AtCAT, AtRD29A, AtRD29B, and AtDREB2A than in control plants. Conclusions Our findings indicate that TaWRKY31 enhances drought resistance in plants by promoting the scavenging of reactive oxygen species, reducing stomatal opening, and increasing the expression levels of stress-related genes.

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“…Recent transcriptome studies have shown that herbaceous plants, such as wheat (Triticum aestivum L.) [33], licorice (Glycyrrhiza glabra L.) [34], broad bean (Vicia faba L.) [35], and millet (Panicum miliaceum) [36] upregulate WRKY TFs in response to drought stress. WRKY TFs in various woody plants, including Myrothamnus flabellifolia [37], and oil palm (Elaeis guineensis Jacq.) [38], also respond to drought stress.…”

Section: Introductionmentioning

confidence: 99%

Functional Study of Amorpha fruticosa WRKY20 Gene in Response to Drought Stress

Li,

Gu,

Huang

et al. 2023

IJMS

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The WRKY gene family in plants regulates the plant’s response to drought through regulatory networks and hormone signaling. AfWRKY20 (MT859405) was cloned from Amorpha fruticosa (A. fruticosa) seedlings using RT-PCR. The binding properties of the AfWRKY20 protein and the W-box (a DNA cis-acting element) were verified both in vivo and in vitro using EMSA and Dual-Luciferase activity assays. RT-qPCR detected that the total expression level of AfWRKY20 in leaves and roots was 22 times higher in the 30% PEG6000 simulated drought treatment compared to the untreated group. Under the simulated drought stress treatments of sorbitol and abscisic acid (ABA), the transgenic tobacco with the AfWRKY20 gene showed enhanced drought resistance at the germination stage, with significantly increased germination rate, green leaf rate, fresh weight, and root length compared to the wild-type (WT) tobacco. In addition, the superoxide dismutase (SOD) activity, chlorophyll content, and Fv/Fm ratio of AfWRKY20 transgenic tobacco were significantly higher than those of the WT tobacco under natural drought stress, while the malondialdehyde (MDA) content and 3,3′-diaminobenzidine (DAB) and nitroblue tetrazolium (NBT) staining levels were lower. The expression levels of oxidation kinase genes (NbSOD, NbPOD, and NbCAT) in transgenic tobacco under drought stress were significantly higher than those in WT tobacco. This enhancement in gene expression improved the ability of transgenic tobacco to detoxify reactive oxygen species (ROS). The survival rate of transgenic tobacco after natural drought rehydration was four times higher than that of WT tobacco. In summary, this study revealed the regulatory mechanism of AfWRKY20 in response to drought stress-induced ABA signaling, particularly in relation to ROS. This finding provides a theoretical basis for understanding the pathways of WRKY20 involved in drought stress, and offers genetic resources for molecular plant breeding aimed at enhancing drought resistance.

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Overexpression of Myrothamnus flabellifolia MfWRKY41 confers drought and salinity tolerance by enhancing root system and antioxidation ability in Arabidopsis

Cited by 9 publications

References 51 publications

Allelic variations of WAK106‐E2Fa‐DPb1‐UGT74E2 module regulate fibre properties in Populus tomentosa

Allelic variations of WAK106‐E2Fa‐DPb1‐UGT74E2 module regulate fibre properties in Populus tomentosa

TaWRKY31, a novel WRKY transcription factor in wheat, participates in regulation of plant drought stress tolerance

Functional Study of Amorpha fruticosa WRKY20 Gene in Response to Drought Stress

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