Overexpression of nicotinamide mononucleotide adenylyltransferase (nmnat) increases the growth rate, Ca2+ concentration and cellulase production in Ganoderma lucidum

Wang, Shengli; Han, Jing; Xia, Jiale; Hu, Yanru; Shi, Liang; Ren, Ang; Zhu, Jing; Zhao, Mingwen

doi:10.1007/s00253-020-10763-0

Cited by 11 publications

(9 citation statements)

References 62 publications

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“…Furthermore, pharmacological experiments, such as exogenous addition of CaCl 2 and the Ca 2+ antagonist LaCl 3 , demonstrated that the Ca 2+ promotes the regulation of cellulase and xylanase activities by GlSwi6. This phenomenon is similar to that in the study by Wang [15]. However, the specific regulatory mechanism of GlSwi6 on cellulase and xylanase activities is still unclear.…”

Section: Discussionsupporting

confidence: 89%

“…The role of the Ca 2+ -responsive signaling pathway was shown to play a role in regulating the expression and secretion of cellulase in Trichoderma reesei Rut-C30; the calcineurin-responsive zinc finger transcription factor Crz binding to the promoters of Cbh1 and Xyr1 in response to 2+ [14]. Further study also found that NAD+ mediated the regulation of cellulase genes through modulating cytosolic Ca 2+ [15]. Ca 2+ signaling and the MAPK signaling pathways have been found play an essential role in this process [38].…”

Section: Discussionmentioning

confidence: 92%

“…Previous studies in G. lucidum indicated that Ca 2+ plays an important role in cellulase production [15]. Therefore, we measured the cytosolic Ca 2+ level in the GlSwi6-silenced and overexpression strains using the Ca 2+ -fluorescent probe Fluo-3AM.…”

Section: Glswi6 Regulates the Cytosolic Ca 2+ Contentmentioning

confidence: 97%

“…The addition of Ca 2+ enhanced the cellulase production [14]. Wang's study provided evidence that Ca 2+ signaling participates in the regulation of cellulase production by increasing intracellular NAD + content [15]. Furthermore, the MAPK pathway in ascomycetes are also reported to be involved in the regulation of cellulolytic enzyme synthesis.…”

Section: Introductionmentioning

confidence: 96%

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GlSwi6 Positively Regulates Cellulase and Xylanase Activities through Intracellular Ca2+ Signaling in Ganoderma lucidum

Lian

Shi

Zhu

et al. 2022

JoF

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Ganoderma lucidum is a white-rot fungus that produces a range of lignocellulolytic enzymes to decompose lignin and cellulose. The mitogen-activated protein kinase (MAPK) pathway has been implicated in xylanases and cellulases production. As the downstream transcription factor of Slt2-MAPK, the function of Swi6 in G. lucidum has not been fully studied. In this study, the transcription factor GlSwi6 in G. lucidum was characterized and shown to significantly positively regulate cellulases and xylanases production. Knockdown of the GlSwi6 gene decreased the activities of cellulases and xylanases by approximately 31%~38% and 54%~60% compared with those of the wild-type (WT) strain, respectively. Besides, GlSwi6 can be alternatively spliced into two isoforms, GlSwi6A and GlSwi6B, and overexpression of GlSwi6B increased the activities of cellulase and xylanase by approximately 50% and 60%, respectively. Further study indicates that the existence of GlSwi6B significantly increased the concentration of cytosolic Ca2+. Our study indicated that GlSwi6 promotes the activities of cellulase and xylanase by regulating the Ca2+ signaling. These results connected the GlSwi6 and Ca2+ signaling in the regulation of cellulose degradation, and provide an insight for further improvement of cellulase or xylanase activities in G. lucidum as well as other fungi.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 92%

Section: Glswi6 Regulates the Cytosolic Ca 2+ Contentmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 96%

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GlSwi6 Positively Regulates Cellulase and Xylanase Activities through Intracellular Ca2+ Signaling in Ganoderma lucidum

Lian

Shi

Zhu

et al. 2022

JoF

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“…The growth of filamentous fungi is regulated in many ways, such as by actin‐like protein (AcnA), ROS, and calcium signals, which play important roles in maintaining the apical dominance of Aspergillus nidulans mycelia [40,41]. Similar to our previous findings, the APSES transcription factor GlSwi6 and nicotinamide mononucleotide adenylate transferase (NMNAT) in G. lucidum can regulate the growth efficiency of G. lucidum , whose growth is also regulated by ROS and Ca 2+ signaling [41–43]. Although studies on the functional regulation of fungal growth and metabolism by HO have not been carried out, in S. cerevisiae , the absence of HMX1 prolongs cell cycle metabolism, for which a lack of CO signaling may be responsible [44].…”

Section: Discussionsupporting

confidence: 53%

Effect of the heme oxygenase gene on mycelial growth and polysaccharide synthesis in Ganoderma lucidum

Wang

Chen

et al. 2021

J Basic Microbiol

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The heme oxygenase gene has antioxidant and cytoprotective effects in organisms, but no related research has been conducted in Ganoderma lucidum. For the first time, we cloned the HMX1 gene in G. lucidum. The CDS is 1092 bp in length and encodes 363 amino acids. The HMX1 protein was prokaryotically expressed and purified, and the enzyme activity of the purified protein was measured. The value of Km was 0.699 μM, and Vm was 81.9 nmol BV h−1 nmol−1 protein. By constructing the silencing vector pAN7‐dual‐HMX1i, the transformants HMX1i1 and HMX1i2 were obtained. Compared with the wild‐type (WT), the average growth rate of HMX1i1 and HMX1i2 decreased by 31% and 23%, respectively, and the mycelium biomass decreased by 53% and 48%, respectively. Compared with the WT, the extracellular polysaccharide content of HMX1i1 and HMX1i2 increased by 59% and 51%, and the intracellular polysaccharide content increased by 24% and 22%, respectively. These results indicate that the HMX1 gene affects mycelial growth and polysaccharide synthesis in G. lucidum.

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Research Progress on Edible Fungi Genetic System

Shi

Zhu

et al. 2022

Advances in Biochemical Engineering/Biotechnology

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Overexpression of nicotinamide mononucleotide adenylyltransferase (nmnat) increases the growth rate, Ca2+ concentration and cellulase production in Ganoderma lucidum

Cited by 11 publications

References 62 publications

GlSwi6 Positively Regulates Cellulase and Xylanase Activities through Intracellular Ca2+ Signaling in Ganoderma lucidum

GlSwi6 Positively Regulates Cellulase and Xylanase Activities through Intracellular Ca2+ Signaling in Ganoderma lucidum

Effect of the heme oxygenase gene on mycelial growth and polysaccharide synthesis in Ganoderma lucidum

Research Progress on Edible Fungi Genetic System

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