Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress

Song, Hongmiao; Fan, Pengxiang; Li, Yinxin

doi:10.1007/s11105-009-0091-6

Cited by 42 publications

(26 citation statements)

References 31 publications

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“…Overexpression of Hsp90C in Arabidopsis reduces tolerance to salt, drought, and oxidative stress (43,44). Additionally, the cr88 mutant, a Gly-to-Arg substitution at position 646 of Hsp90C in Arabidopsis, alters responses to red light and constitutively delays chloroplast development (18).…”

Section: Discussionmentioning

confidence: 99%

An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts

Inoue

Schnell

2013

Proc. Natl. Acad. Sci. U.S.A.

108

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Chloroplast heat shock protein 90 (Hsp90C) represents a highly conserved subfamily of the Hsp90 family of molecular chaperones whose function has not been defined. We identified Hsp90C as a component that interacts with import intermediates of nuclear-encoded preproteins during posttranslational import into isolated chloroplasts. Hsp90C was specifically coprecipitated with a complex of protein import components, including Tic110, Tic40, Toc75, Tic22, and the stromal chaperones, Hsp93 and Hsp70. Radicicol, an inhibitor of Hsp90 ATPase activity, reversibly inhibited the import of a variety of preproteins during translocation across the inner envelope membrane, indicating that Hsp90C functions in membrane translocation into the organelle. Hsp90C is encoded by a single gene in Arabidopsis thaliana, and insertion mutations in the Hsp90C gene are embryo lethal, indicating an essential function for the chaperone in plant viability. On the basis of these results, we propose that Hsp90C functions within a chaperone complex in the chloroplast stroma to facilitate membrane translocation during protein import into the organelle.

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Section: Discussionmentioning

confidence: 99%

An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts

Inoue

Schnell

2013

Proc. Natl. Acad. Sci. U.S.A.

108

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“…Reactive oxygen species (ROS) have been implicated in both signaling and triggering of PCD (Burbridge et al 2006;George and Parida 2010;Song et al 2009). Hydrogen peroxide (H 2 O 2 ) generated by various environmental and developmental stimuli can act as a signaling molecule that regulates plant development, stress adaptation, and PCD (Apel and Hirt 2004;Gao et al 2010).…”

Section: Ospdcd5-induced Cell Death Exhibited Multiple Pcd Featuresmentioning

confidence: 99%

Investigating the Role of OsPDCD5, a Homolog of the Mammalian PDCD5, in Programmed Cell Death by Inducible Expression in Rice

Sun

Qian

et al. 2011

Plant Mol Biol Rep

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OsPDCD5 is the rice (Oryza sativa L.) homolog of PDCD5, a gene involved in programmed cell death (PCD) regulation and highly conserved over time. PDCD5 overexpression in tumor cells enhances apoptosis triggered by growth factor or serum deprivation. In our previous research, constitutive OsPDCD5 overexpression induces early death in transgenic plants. In this study, an inducible OsPDCD5 expression strategy was employed to systematically study the role of OsPDCD5 in rice. With this system, OsPDCD5 was found to be capable of independently inducing cell death in three-leaf stage and older seedlings. These altered plants exhibited lesion mimic phenotype, abnormal leaf morphology, DNA fragmentation, hydrogen peroxide production, and mitochondrial distortion. In nature, RNA hybridization in situ has shown that OsPDCD5 expression was predominantly localized to the tapetal layer where PCD occurred. Transcript microarray analyses here revealed that many PCD-related genes were involved. These data, taken together, indicated that OsPDCD5-induced cell death is a kind of PCD. Nevertheless, OsPDCD5 failed to induce any visible morphological phenotypes in two-leaf stage and younger seedlings. Transcript microarray analyses and quantitative real-time polymerase chain reaction showed that the Bax inhibitor-1 (BI-1) mRNA concentration and the activity of an ubiquitin gene were specifically changed. Endogenous OsPDCD5 upregulation, induced by ectopic OsPDCD5 expression in three-leaf stage seedlings, was also absent in two-leaf stage seedlings, suggesting strongly that young seedlings could inhibit PCD at some level.

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“…Previous studies revealed that the Hsp90 gene not only is induced by abiotic stresses such as saline-alkaline, high temperatures, low temperatures, and heavy metals (Pareek et al, 1995;Song et al, 2009), but also is involved in the plants' resistance to pathogens. Wang et al (2011) found that wheat plants over-expressing the Hsp90 gene family members TaHsp90.2 and TaHsp90.3 showed significant resistance to stripe rust.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum

et al. 2015

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ABSTRACT. Heat shock protein 90 (Hsp90) is a protein produced by plants in response to adverse environmental stresses. In this study, we identified and analyzed Hsp90 gene family members using a bioinformatic method based on genomic data from tomato (Solanum lycopersicum L.). The results illustrated that tomato contains at least 7 Hsp90 genes distributed on 6 chromosomes; protein lengths ranged from 267-794 amino acids. Intron numbers ranged from 2-19 in the genes. The phylogenetic tree revealed that Hsp90 genes in tomato (Solanum lycopersicum L.), rice (Oryza sativa L.), and Arabidopsis (Arabidopsis thaliana L.) could be divided into 5 groups, which included 3 pairs of orthologous genes and 4 pairs of paralogous genes. Expression analysis of RNA-sequence data showed that the Hsp90-1 gene was specifically expressed in mature fruits, while Hsp90-5 and Hsp90-6 showed 7812 W.S. Zai et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 7811-7820 (2015) opposite expression patterns in various tissues of cultivated and wild tomatoes. The expression levels of the Hsp90-1, Hsp90-2, and Hsp90-3 genes in various tissues of cultivated tomatoes were high, while both the expression levels of genes Hsp90-3 and Hsp90-4 were low. Additionally, quantitative real-time polymerase chain reaction showed that these genes were involved in the responses to yellow leaf curl virus in tomato plant leaves. Our results provide a foundation for identifying the function of the Hsp90 gene in tomato.

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Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress

Cited by 42 publications

References 31 publications

An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts

An essential role for chloroplast heat shock protein 90 (Hsp90C) in protein import into chloroplasts

Investigating the Role of OsPDCD5, a Homolog of the Mammalian PDCD5, in Programmed Cell Death by Inducible Expression in Rice

Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum

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