Overexpression of Rsf-1 correlates with poor survival and promotes invasion in non-small cell lung cancer

Abstract

Rsf-1 (HBXAP) was recently reported to play roles in tumorigenesis and tumor progression. There have been many reports referred to Rsf-1 overexpression in various cancers and associated with the malignant behavior of cancer cells. However, the molecular mechanism of Rsf-1 in non-small cell lung cancer aggressiveness remains ambiguous. In the present study, we found that there was a significant association between Rsf-1 overexpression and poor overall survival (p = 0.028) in lung cancer. Furthermore, knockdown of Rsf-1 expression in H1299 and H460 cells with high endogenous Rsf-1 expression inhibited cell migration and invasion and downregulated MMP2 expression and nuclear levels of NF-κB. NF-κB inhibitor could also block the effect of Rsf-1 in regulation of MMP2 expression. Further experiments demonstrated that Rsf-1 depletion restrained NF-κB reporter luciferase activity and downregulated bcl-2 and p-IκB protein level. In conclusion, we demonstrated that Rsf-1 was overexpressed in lung cancer and associated with poor survival. Rsf-1 regulated cell invasion through MMP2 and NF-κB pathway.

Remodeling and spacing factor 1 overexpression is associated with poor prognosis in renal cell carcinoma

“…The results, suggesting that high RSF1 expression indicates a poor prognosis of RCC, are consistent with data in ovarian, prostate and hepatocellular cancer (17,18,21). The results may be explained by previous studies demonstrating that RSF1 promotes tumor cell proliferation and invasion (10,13,14,16). All of these results together suggest that RSF1 is an important oncogene in RCC.…”

“…The genes of the region include RSF1, EMSY, BRCA2 interacting transcriptional repressor, P21 (RAC1) activated kinase 1 (PAK1), RAD9 checkpoint clamp component A, and cyclin D1 have been proposed as candidate tumor driver genes (12,13,15,(23)(24)(25)(26). Numerous studies have demonstrated that the amplification of RSF1 is not only significantly correlated with tumor cell proliferation, migration, invasion and apoptosis abilities, but also with poor prognosis of patients with ovarian, lung, hepatocellular and prostate cancer (14)(15)(16)(17)(18).…”

“…RSF1 is overexpressed and exhibits gene amplification in multiple solid tumors, including breast cancer, ovarian cancer and oral squamous cell carcinoma (11)(12)(13). Studies have indicated that overexpression and/or gene amplification of RSF1 in tumors is associated with proliferation, invasion and poor prognosis (14)(15)(16)(17)(18). Accumulating evidence suggests that 11q13 region gene amplification is closely associated with the development of malignant tumors (14)(15)(16)(17)(18).…”

“…Studies have indicated that overexpression and/or gene amplification of RSF1 in tumors is associated with proliferation, invasion and poor prognosis (14)(15)(16)(17)(18). Accumulating evidence suggests that 11q13 region gene amplification is closely associated with the development of malignant tumors (14)(15)(16)(17)(18). An oncogene in the 11q13 region is also overexpressed in RCC (19).…”

Rsf-1 Influences the Sensitivity of Non-Small Cell Lung Cancer to Paclitaxel by Regulating NF-κB Pathway and Its Downstream Proteins

“…Rsf-1 encodes a histone-binding protein named remodeling and spacing factor 1 (Rsf-1), which was found to interact with human sucrose nonfermenting protein 2 homologue (hSNF2H) to form an ISWI chromatin remodeling complex [8], which is involved in chromatin remodeling and transcriptional regulation [9]. Rsf-1 is amplified and/or overexpressed in various cancers, including ovarian [7, 8], breast [10], bladder [11], esophageal [12], lung [13], colon [14], and head and neck cancers [15]. Elevated levels of Rsf-1 are correlated with poor prognosis [16].…”