Overexpression of the ABA-Dependent AREB1 Transcription Factor from Arabidopsis thaliana Improves Soybean Tolerance to Water Deficit

Barbosa, Elton Gargioni Grisoste; Leite, João P.; Marin, Silvana Regina Rockenbach; Marinho, Juliane Prela; Carvalho, Josirley de Fátima Corrêa; Fuganti-Pagliarini, Renata; Farias, J. R. B.; Neumaier, N.; Marcelino-Guimarães, Francismar Corrêa; Oliveira, M. C. N. de; Yamaguchi-Shinozaki, Kazuko; Nakashima, Kenichiro; Maruyama, Kyonoshin; Kanamori, Norihito; Fujita, Yasunari; Yoshida, Takuya; Nepomuceno, Alexandre Lima

doi:10.1007/s11105-012-0541-4

Cited by 67 publications

(45 citation statements)

References 43 publications

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“…Stunted growth is the common phenotype observed when a TF gene is overexpressed in transgenic plants. This has been observed in soybean (Barbosa et al, 2013), tomato (Pan et al, 2012), rice (Wu et al, 2009), tobacco (Kasuga et al, 2004), and chrysanthemum (Hong et al, 2006). With the selection of different promoters, it is possible to express transgenes in defined temporal and spatial manners.…”

Section: Mechanisms Of Regulation Of Different Classes Of Tfsmentioning

confidence: 95%

“…However, lower water loss rate was not due to stomatal density and aperture since no significant difference was observed between overexpressed and knockout lines under normal and drought conditions (Xiang et al, 2008). Heterologous expression of Arabidopsis bZIP genes also showed a phenotype with increased drought tolerance in bentgrass (Choi et al, 2013) and soybean (Barbosa et al, 2013).…”

Section: Oswrky30mentioning

confidence: 95%

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The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Rabara¹,

Tripathi

Rushton³

2014

OMICS: A Journal of Integrative Biology

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Drought is one of the major constraints in crop production and has an effect on a global scale. In order to improve crop production, it is necessary to understand how plants respond to stress. A good understanding of regulatory mechanisms involved in plant responses during drought will enable researchers to explore and manipulate key regulatory points in order to enhance stress tolerance in crops. Transcription factors (TFs) have played an important role in crop improvement from the dawn of agriculture. TFs are therefore good candidates for genetic engineering to improve crop tolerance to drought because of their role as master regulators of clusters of genes. Many families of TFs, such as CCAAT, homeodomain, bHLH, NAC, AP2/ERF, bZIP, and WRKY have members that may have the potential to be tools for improving crop tolerance to drought. In this review, the roles of TFs as tools to improve drought tolerance in crops are discussed. The review also focuses on current strategies in the use of TFs, with emphasis on several major TF families in improving drought tolerance of major crops. Finally, many promising transgenic lines that may have improved drought responses have been poorly characterized and consequently their usefulness in the field is uncertain. New advances in high-throughput phenotyping, both greenhouse and field based, should facilitate improved phenomics of transgenic lines. Systems biology approaches should then define the underlying changes that result in higher yields under water stress conditions. These new technologies should help show whether manipulating TFs can have effects on yield under field conditions.

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Section: Mechanisms Of Regulation Of Different Classes Of Tfsmentioning

confidence: 95%

Section: Oswrky30mentioning

confidence: 95%

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Rabara¹,

Tripathi

Rushton³

2014

OMICS: A Journal of Integrative Biology

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“…These activated genes can be classified as genes involved in cellular metabolism, stress tolerance, and regulatory genes including various kinases, phosphatases, and transcription factors (TFs) (Cheong et al, 2003;Agarwal and Jha, 2010). TFs, individually or combined with others factors, play a key role in the activation or repression of signal transduction pathways once they recognize specific DNA sequences in the regulatory regions of target genes regulating their expression (Barbosa et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

“…The AREB1/ABF2 TFs are basic leucine zipper (bZIP) proteins that regulate ABA-dependent stress-responsive gene expression, and they act as a major TF under abiotic stress conditions in A. thaliana (Kobayashi et al, 2008;Lee et al, 2010). Several studies have demonstrated the importance and potential of AREB1 and bZIP proteins in abiotic stress tolerance Gao et al, 2011;Kim et al, 2011;Reeves et al, 2011;Barbosa et al, 2013). However, the overexpression of AREB1 under the control of the cauliflower mosaic virus (CaMV) 35S promoter (35S-AREB1) on its own is insufficient to induce the expression of downstream genes such as RD29B in normal growth conditions because AREB1 requires ABA for full activation.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit

Leite¹,

Barbosa²,

Marin³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. Abscisic acid-responsive element binding protein (AREB1) is a basic domain/leucine zipper transcription factor that binds to the abscisic acid (ABA)-responsive element motif in the promoter 6273 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 13 (3): 6272-6286 (2014) Water deficit response in GM soybeans with AtAREB1∆QT region of ABA-inducible genes. Because AREB1 is not sufficient to direct the expression of downstream genes under non-stress conditions, an activated form of AREB1 (AREB1∆QT) was created. Several reports claim that plants overexpressing AREB1 or AREB1∆QT show improved drought tolerance. In our studies, soybean plants overexpressing AREB1∆QT were characterized molecularly, and the phenotype and drought response of three lines were accessed under greenhouse conditions. Under conditions of water deficit, the transformed plants presented a higher survival rate (100%) than those of their isoline, cultivar BR 16 (40%). Moreover, the transformed plants displayed better water use efficiency and had a higher number of leaves than their isoline. Because the transgenic plants had higher stomatal conductance than its isoline under well-watered conditions, it was suggested that the enhanced drought response of AREB1∆QT soybean plants might not be associated with altered transpiration rates mediated by ABA-dependent stomatal closure. However, it is possible that the smaller leaf area of the transgenic plants reduced their transpiration and water use, causing delayed stress onset. The difference in the degree of wilting and percentage of survival between the 35S-AREB1∆QT and wildtype plants may also be related to the regulation of genes that protect against dehydration because metabolic impairment of photosynthesis, deduced by an increasing internal CO 2 concentration, was not observed in the transgenic plants.

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Signaling and Modulation of Non‐CodingRNAs in plants by Abscisic Acid (ABA)

Joshi

Megha

Basu

et al. 2017

Mechanism of Plant Hormone Signaling Under Stress

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Overexpression of the ABA-Dependent AREB1 Transcription Factor from Arabidopsis thaliana Improves Soybean Tolerance to Water Deficit

Cited by 67 publications

References 43 publications

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

The Potential of Transcription Factor-Based Genetic Engineering in Improving Crop Tolerance to Drought

Overexpression of the activated form of the AtAREB1 gene (AtAREB1ΔQT) improves soybean responses to water deficit

Signaling and Modulation of Non‐CodingRNAs in plants by Abscisic Acid (ABA)

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