Overexpression of the actin gene is associated with the morphogenesis of Candida albicans

Paranjape, Vijay; Datta, Asis

doi:10.1016/0006-291x(91)91387-r

Cited by 23 publications

(10 citation statements)

References 8 publications

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“…These results disagree with a recent report by Paranjape and Datta that actin mRNA levels increase during morphogenesis (38). Our conditions differed from theirs in that we used (i) yeast cells in balanced growth, rather than stationaryphase yeast cells, as an inoculum and (ii) the same temperature and media for growth of yeast and hyphal forms.…”

Section: Discussioncontrasting

confidence: 96%

Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant, immunodominant glycolytic enzyme

Sundstrom

Aliaga

1992

J Bacteriol

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We isolated and sequenced a clone for Candida albicans enolase from a C. albicans cDNA library by using molecular genetic techniques. The 1.4-kbp cDNA encoded one long open reading frame of 440 amino acids which was 87 and 75% similar to predicted enolases of Saccharomyces cerevisiae and enolases from other organisms, respectively. The cDNA included the entire coding region and predicted a protein of molecular weight 47,178. The codon usage was highly biased and similar to that found for the highly expressed EF-1 alpha proteins of C. albicans. Northern (RNA) blot analysis showed that the enolase cDNA hybridized to an abundant C. albicans mRNA of 1.5 kb present in both yeast and hyphal growth forms. The polypeptide product of the cloned cDNA, which was purified as a recombinant protein fused to glutathione S-transferase, had enolase enzymatic activity and inhibited radioimmunoprecipitation of a single C. albicans protein of molecular weight 47,000. Analysis of the predicted C. albicans enolase showed strong conservation in regions of alpha helices, beta sheets, and beta turns, as determined by comparison with the crystal structure of apo-enolase A of S. cerevisiae. The lack of cysteine residues and a two-amino-acid insertion in the main domain differentiated C. albicans enolase from S. cerevisiae enolase. Immunofluorescence of whole C. albicans cells by using a mouse antiserum generated against the purified fusion protein showed that enolase is not located on the surface of C. albicans. Recombinant C. albicans enolase will be useful in understanding the pathogenesis and host immune response in disseminated candidiasis, since enolase is an immunodominant antigen which circulates during disseminated infections.

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Section: Discussioncontrasting

confidence: 96%

Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant, immunodominant glycolytic enzyme

Sundstrom

Aliaga

1992

J Bacteriol

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“…However, ACT1 mRNA levels are known to change during the yeast-to-hyphal transition in C. albicans (Lasker & Riggsby, 1992;Paranjape & Datta, 1991 ;Delbruck & Ernst, 1993), and therefore in this study we investigated TEF3 expression in more detail with a view to testing the possibility of using this mRNA as a loading control in studies of dimorphic gene regulation. We measured TEF3 mRNA levels relative to the ribosomal RNAs as a loading control in the Northern blots.…”

Section: Discussionmentioning

confidence: 99%

“…been shown to change during morphogenesis in C. albicans (Lasker & Riggsby, 1992;Paranjape & Datta, 1991;Delbruck & Ernst, 1993). Consistent with previous reports, ACT1 mRNA levels increased upon dilution of late-exponential cells into fresh medium, thus validating the observed increase in TEF3 mRNA levels.…”

Section: T€f3 Mrna Levels During Morphogenesismentioning

confidence: 99%

Regulation of the gene encoding translation elongation factor 3 during growth and morphogenesis in Candida albicans

et al. 1994

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The level of the T€F3 mRNA, which encodes the fungal-specific translation elongation factor 3 (EF-3), was measured during the yeast-to-hyphal transition in Candida albicans. In contrast to a previous report, T€F3 mRNA levels were shown to change during dilution into fresh medium, increasing only transiently when dimorphism was induced by either (i) an increase in growth temperature (from 25 "C to 37 "C) combined with the addition of 10% (vh) bovine calf serum to the medium, or (ii) an increase in growth temperature (from 25 "C to 37 "C) combined with an increase in the pH of the medium (from pH 4 5 to 6.5). T€F3 mRNA levels also increased in control cultures under conditions where germ tubes were not formed, but they remained elevated in contrast t o cultures undergoing morphological changes. E F 3 mRNA levels were not significantly affected by heat-shock, but were tightly regulated during batch growth of the yeast form, reaching maximal levels in exponential phase. Therefore, the changes in T€F3 expression that accompany the dimorphic transition in C. albicans appear to reflect the underlying physiological changes that occur during morphogenesis and are not a response to morphogenesis per se. For this reason T€F3 mRNA measurement cannot be used as a loading control in Northern analyses of dimorphic gene regulation. Comparison of TEF3 mRNA levels with the abundance of the EF-3 polypeptide indicated that the synthesis of this essential translation factor might be subject to post-transcriptional regulation.

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“…Microfilaments, but not microtubules, appear to be required for the elongation of filamentous cell tips (Yokoyama etai, 1990). Recently, a correlation between hyphal formation and the mRNA level of the actin-encoding gene, ACT1 (Losberger and Ernst, 1989b), has been discovered {Paranjape and Datta, 1991;Lasker and Riggsby, 1992). However, a fusion of the ACT1 promoter to the Kluyveromyces iactis LAC4 gene was not differentially expressed during the yeast and hyphal growth modes of C. albicans (Leuker ef ai., 1992).…”

Section: Introductionmentioning

confidence: 99%

Morphogenesis‐independent regulation of actin transcript levels in the pathogenic yeast Candida albicans

Delbrück¹,

Ernst²

1993

Molecular Microbiology

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The transcript level of the Candida albicans ACT1 gene (encoding actin) is strongly regulated during induction of hyphal morphogenesis. ACT1 mRNA declines rapidly during starvation pretreatment and quickly recovers in media inducing morphogenesis. The C. albicans URA3 and LEU2 mRNAs, as well as an ACT1 promoter/LAC4 fusion, are regulated similarly. The regulation of ACT1/LAC4 and unaltered mRNA stabilities suggest transcriptional regulation during morphogenesis. However, by individually testing morphogenesis induction parameters, it is shown that starvation and growth phase, but not hyphal formation, are responsible for ACT1 transcript regulation; this conclusion is confirmed by analyses of morphological mutants and by inhibition of hyphal development. Thus, the specific morphogenesis-induction conditions, but not morphogenesis per se, affect transcript levels in C. albicans.

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Overexpression of the actin gene is associated with the morphogenesis of Candida albicans

Cited by 23 publications

References 8 publications

Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant, immunodominant glycolytic enzyme

Molecular cloning of cDNA and analysis of protein secondary structure of Candida albicans enolase, an abundant, immunodominant glycolytic enzyme

Regulation of the gene encoding translation elongation factor 3 during growth and morphogenesis in Candida albicans

Morphogenesis‐independent regulation of actin transcript levels in the pathogenic yeast Candida albicans

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