Overexpression of the drug resistance‐associated protein metallothionein does not correlate with response of sarcomas to isolated limb perfusion treatment

Grabellus, Florian; Sheu, Sien Yi; Tötsch, Martin; Lehmann, Nils; Kaiser, G.; Jasani, Bharat; Taeger, G.; Schmid, Kurt Werner

doi:10.1002/jso.21513

Cited by 9 publications

(7 citation statements)

References 33 publications

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“…MT was detected both by immunohistochemistry and by real time (RT) PCR. 66,205 Similar results were described by Hishikawa and co-workers in oesophageal cancer. They found more frequent 5-year survival of patients suffering from squamous cell oesophageal cancer treated with cisplatin in MT negative than MT positive tumours.…”

Section: Clinical Relevance Of Mtsupporting

confidence: 83%

Mammalian metallothioneins: properties and functions

et al. 2012

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Metallothioneins (MT) are a family of ubiquitous proteins, whose role is still discussed in numerous papers, but their affinity to some metal ions is undisputable. These cysteine-rich proteins are connected with antioxidant activity and protective effects on biomolecules against free radicals, especially reactive oxygen species. In this review, the connection between zinc(II) ions, reactive oxygen species, heavy metal ions and metallothioneins is demonstrated with respect to effect of these proteins on cell proliferation and a possible negative role in resistance to heavy metal-based and non-heavy metal-based drugs.

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Section: Clinical Relevance Of Mtsupporting

confidence: 83%

Mammalian metallothioneins: properties and functions

et al. 2012

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“…Most of these have focused on adults [6,18,29–33] and far fewer on children [34–37]. Studies in adults have shown that cellular MT could serve as prognostic markers in some tumours [17,38–43]. Relationships have been described between MT and p53 status [1], increased tumour grade [44,45] and metastases development [1,46].…”

Section: Discussionmentioning

confidence: 99%

Serum Metallothioneins in Childhood Tumours — A Potential Prognostic Marker

Kruseová

Hynek

Adam

et al. 2013

IJMS

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Metallothioneins (MT) are low molecular weight, cysteine-rich proteins maintaining metal ions homeostasis. They play a role in carcinogenesis and may also cause chemoresistance. The aim of the study was to explore the importance of MT serum levels in children suffering from malignant tumours. This prospective study involves examination of 865 samples from 172 patients with malignant tumours treated from 2008 to 2011 at University Hospital Motol. MT serum levels were determined using differential pulse voltammetry–Brdicka reaction. Mean MT level was 2.7 ± 0.5 μM. There was no statistically significant difference between MT levels in different tumours. We also did not find any correlation between MT levels and response to therapy or clinical stages. However, we found a positive correlation between MT levels and age (p = 0.009) and a negative correlation with absolute lymphocyte number (p = 0.001). The fact that patients who had early disease recurrence had lower MT levels during the treatment (complete remission 2.67 vs. recurring 2.34, p = 0.001) seems to be important for clinical practice. Accordingly we believe that there is benefit in further studies of serum MT levels in tumours.

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“…to the MTs level have been published [214]. On the other hand in some other studies devoted to colorectal and bladder cancer and others, no significant correlation between MTs expression and prognosis was observed [215][216][217]. The relationship between MTs and cancer has been discussed in details recently in review by Eckschlager et al [218].…”

Section: Metallothionein and Tumor Diseasementioning

confidence: 99%

Analytical Methods for Metallothionein Detection

Ryvolová¹,

Křížková²,

Adam³

et al. 2011

CAC

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Metallothioneins (MTs) are a group of low molecular mass, cysteine-rich proteins with a variety of functions including involvement in metal homeostasis, free radical scavenging, protection against heavy metal damage, and metabolic regulation via Zn donation. The overexpression of MTs in proliferating cells is turning the attention to the study of MTs as novel promising marker of tumor diseases. Besides, levels of MTs in invertebrates and aquatic vertebrates well correlate with heavy metal pollution of an environment and, thus, serve as bio-environmental marker. It is not surprising that these proteins are of great interest not only for biochemists, molecular biologists clinical chemists, but also for environmental chemists and ecologists.Detection and quantification of MTs, which is needed in all above mentioned areas, is challenging due to the high cysteine content and relatively low molecular mass. This review is giving an overview of the analytical methods used for determination of MTs comprising a broad range of immunochemical, electrochemical as well as spectrometric methods, which have been optimized and even hyphenated with different separation methods to detect MTs. More attention is paid to the structural analysis of these proteins as well as the employment of analytical instruments for environmental, biochemical and clinical purposes.

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Overexpression of the drug resistance‐associated protein metallothionein does not correlate with response of sarcomas to isolated limb perfusion treatment

Cited by 9 publications

References 33 publications

Mammalian metallothioneins: properties and functions

Mammalian metallothioneins: properties and functions

Serum Metallothioneins in Childhood Tumours — A Potential Prognostic Marker

Analytical Methods for Metallothionein Detection

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