Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco

Huo, Yongjin; Wang, Meiping; Wei, Yangyang; Zhang, Xia

doi:10.3389/fpls.2015.01223

Cited by 85 publications

(66 citation statements)

References 47 publications

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“…In addition, psbA protects photosystem II from oxidative stress developed through drought. A previous report of Huo et al [60] demonstrated that the overexpression of the psbA gene improves drought tolerance in tobacco plant. The occurrence of psbA in B .…”

Section: Resultsmentioning

confidence: 99%

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

et al. 2017

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Boswellia sacra (Burseraceae), a keystone endemic species, is famous for the production of fragrant oleo-gum resin. However, the genetic make-up especially the genomic information about chloroplast is still unknown. Here, we described for the first time the chloroplast (cp) genome of B. sacra. The complete cp sequence revealed a circular genome of 160,543 bp size with 37.61% GC content. The cp genome is a typical quadripartite chloroplast structure with inverted repeats (IRs 26,763 bp) separated by small single copy (SSC; 18,962 bp) and large single copy (LSC; 88,055 bp) regions. De novo assembly and annotation showed the presence of 114 unique genes with 83 protein-coding regions. The phylogenetic analysis revealed that the B. sacra cp genome is closely related to the cp genome of Azadirachta indica and Citrus sinensis, while most of the syntenic differences were found in the non-coding regions. The pairwise distance among 76 shared genes of B. sacra and A. indica was highest for atpA, rpl2, rps12 and ycf1. The cp genome of B. sacra reveals a novel genome, which could be used for further studied to understand its diversity, taxonomy and phylogeny.

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Section: Resultsmentioning

confidence: 99%

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

et al. 2017

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“…While most photosynthesis‐related genes were downregulated after dehydration, especially in TPs, several photosynthesis‐related genes were upregulated, such as PsbA , PsbE , PsbN and Psb29.4 (Figure S3). These genes were upregulated probably for self‐protection, such as oxidation detoxification, recovery from photoinhibition and response to excess light (Keren et al ., ; Torabi et al ., ; Sugiura et al ., ; Huo et al ., ).…”

Section: Discussionmentioning

confidence: 97%

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

et al. 2019

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† These authors contributed equally to this work. SUMMARYWRKY transcription factors play important roles in response to various abiotic stresses. Previous study have proved that soybean GmWRKY54 can improve stress tolerance in transgenic Arabidopsis. Here, we generated soybean transgenic plants and further investigated roles and biological mechanisms of GmWRKY54 in response to drought stress. We demonstrated that expression of GmWRKY54, driven by either a constitutive promoter (pCm) or a drought-induced promoter (RD29a), confers drought tolerance. GmWRKY54 is a transcriptional activator and affects a large number of stress-related genes as revealed by RNA sequencing. Gene ontology (GO) enrichment and co-expression network analysis, together with measurement of physiological parameters, supported the idea that GmWRKY54 enhances stomatal closure to reduce water loss, and therefore confers drought tolerance in soybean. GmWRKY54 directly binds to the promoter regions of genes including PYL8, SRK2A, CIPK11 and CPK3 and activates them. Therefore GmWRKY54 achieves its function through abscisic acid (ABA) and Ca 2+ signaling pathways. It is valuable that GmWRKY54 activates an ABA receptor and an SnRK2 kinase in the upstream position, unlike other WRKY proteins that regulate downstream genes in the ABA pathway. Our study revealed the role of GmWRKY54 in drought tolerance and further manipulation of this gene should improve growth and production in soybean and other legumes/crops under unfavorable conditions.

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“…MDA content was determined as described previously (Draper and Hadley, 1990; Huo et al, 2016). H 2 O 2 content was assayed according to our previously used method (Xia et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Impairment of Sulfite Reductase Decreases Oxidative Stress Tolerance in Arabidopsis thaliana

Wang

Jia

et al. 2016

Front. Plant Sci.

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As an essential enzyme in the sulfate assimilation reductive pathway, sulfite reductase (SiR) plays important roles in diverse metabolic processes such as sulfur homeostasis and cysteine metabolism. However, whether plant SiR is involved in oxidative stress response is largely unknown. Here, we show that SiR functions in methyl viologen (MV)-induced oxidative stress in Arabidopsis. The transcript levels of SiR were higher in leaves, immature siliques, and roots and were markedly and rapidly up-regulated by MV exposure. The SiR knock-down transgenic lines had about 60% residual transcripts and were more susceptible than wild-type when exposed to oxidative stress. The severe damage phenotypes of the SiR-impaired lines were accompanied by increases of hydrogen peroxide (H2O2), malondialdehyde (MDA), and sulfite accumulations, but less amounts of glutathione (GSH). Interestingly, application of exogenous GSH effectively rescued corresponding MV hypersensitivity in SiR-impaired plants. qRT-PCR analysis revealed that there was significantly increased expression of several sulfite metabolism-related genes in SiR-impaired lines. Noticeably, enhanced transcripts of the three APR genes were quite evident in SiR-impaired plants; suggesting that the increased sulfite in the SiR-impaired plants could be a result of the reduced SiR coupled to enhanced APR expression during oxidative stress. Together, our results indicate that SiR is involved in oxidative stress tolerance possibly by maintaining sulfite homeostasis, regulating GSH levels, and modulating sulfite metabolism-related gene expression in Arabidopsis. SiR could be exploited for engineering environmental stress-tolerant plants in molecular breeding of crops.

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Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco

Cited by 85 publications

References 47 publications

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean

Impairment of Sulfite Reductase Decreases Oxidative Stress Tolerance in Arabidopsis thaliana

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Overexpression of the Maize psbA Gene Enhances Drought Tolerance Through Regulating Antioxidant System, Photosynthetic Capability, and Stress Defense Gene Expression in Tobacco

Cited by 85 publications

References 47 publications

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

The First Chloroplast Genome Sequence of Boswellia sacra, a Resin-Producing Plant in Oman

GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca2+ signaling pathways in transgenic soybean

Impairment of Sulfite Reductase Decreases Oxidative Stress Tolerance in Arabidopsis thaliana

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GmWRKY54 improves drought tolerance through activating genes in abscisic acid and Ca²⁺ signaling pathways in transgenic soybean